• Thumbnail for Barnase
    Barnase (a portmanteau of "BActerial" "RiboNucleASE") is a bacterial protein that consists of 110 amino acids and has ribonuclease activity. It is synthesized...
    6 KB (652 words) - 12:45, 13 April 2022
  • Thumbnail for DMH-11 Mustard
    transgenic technology, primarily involving the Bar, Barnase and Barstar gene system. The Barnase gene confers male sterility, while the Barstar gene restores...
    23 KB (2,602 words) - 09:21, 23 February 2024
  • Thumbnail for Protein complex
    The Bacillus amyloliquefaciens ribonuclease barnase (colored) and its inhibitor (blue) in a complex...
    25 KB (2,841 words) - 18:08, 16 November 2024
  • Thumbnail for Alan Fersht
    Fersht LMB Profile Clarke, Jane (1993). Studies of disulphide mutants of barnase. jisc.ac.uk (PhD thesis). University of Cambridge. OCLC 53666398. EThOS uk...
    19 KB (1,690 words) - 10:12, 21 November 2024
  • Thumbnail for Barstar
    activity of its binding partner barnase, with which it forms an extraordinarily tightly bound complex within the cell until barnase is secreted. Expression of...
    2 KB (183 words) - 22:14, 21 December 2023
  • Thumbnail for Bacillus amyloliquefaciens
    BamHI restriction enzyme. It also synthesizes a natural antibiotic protein barnase, a widely studied ribonuclease that forms a famously tight complex with...
    12 KB (1,214 words) - 22:49, 2 December 2023
  • domains, WW domain, individual domains of proteins L and G, ubiquitin, and barnase have all been studied by ϕ {\displaystyle \phi } analysis. Phi is defined...
    13 KB (1,688 words) - 03:29, 27 October 2024
  • Thumbnail for Jane Clarke (scientist)
    Philosophy degree in 1993 for investigations of Bacterial Ribonuclease (Barnase) from the University of Cambridge supervised by Alan Fersht. Clarke was...
    13 KB (1,028 words) - 06:44, 19 November 2024
  • Thumbnail for Bacillus
    Bacillus amyloliquefaciens is the source of a natural antibiotic protein barnase (a ribonuclease), alpha amylase used in starch hydrolysis, the protease...
    53 KB (4,741 words) - 08:15, 13 November 2024
  • Thumbnail for Cloning vector
    This may involve the use of a gene lethal to the host cells, such as barnase, Ccda, and the parD/parE toxins. This typically works by disrupting or...
    20 KB (2,517 words) - 17:11, 5 November 2024
  • Thumbnail for Enzyme inhibitor
    inhibitor proteins include the barstar inhibitor of the bacterial ribonuclease barnase. Animals and plants have evolved to synthesise a vast array of poisonous...
    104 KB (11,579 words) - 19:29, 18 September 2024
  • resulted in the seminal application of phi-value analysis to the study of barnase, a bacterial RNAse used in many protein folding studies. Development of...
    5 KB (402 words) - 11:29, 9 January 2023
  • bacterial gene barnase from transgenic plants. This gene kills any cell that expresses it and lacks barstar, the specific inhibitor of barnase activity. In...
    13 KB (1,475 words) - 16:06, 14 June 2024
  • Thumbnail for Ribonuclease T1
    denatured RNA prior to sequencing. Similar to other ribonucleases such as barnase and RNase A, ribonuclease T1 has been popular for folding studies. Structurally...
    5 KB (454 words) - 21:22, 1 December 2023
  • Thumbnail for Expanded genetic code
    non-natural one. To remove the former, the plasmid is inserted into cells with a barnase gene (toxic) with a premature amber codon but without the non-natural amino...
    79 KB (9,132 words) - 16:16, 17 November 2024
  • S2CID 2130725. Vu, ND; Feng, H; Bai, Y (30 Mar 2004). "The folding pathway of barnase: the rate-limiting transition state and a hidden intermediate under native...
    11 KB (1,365 words) - 10:01, 27 March 2024
  • Thumbnail for List of biophysically important macromolecular crystal structures
    transcriptase 1993 – Beta helix of Pectate lyase 1994 – Collagen 1994 – Barnase/barstar complex 1994 – F1 ATPase 1995 – Heterotrimeric G proteins 1996 –...
    23 KB (2,701 words) - 20:55, 15 June 2024