Barnase (a portmanteau of "BActerial" "RiboNucleASE") is a bacterial protein that consists of 110 amino acids and has ribonuclease activity. It is synthesized...
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transgenic technology, primarily involving the Bar, Barnase and Barstar gene system. The Barnase gene confers male sterility, while the Barstar gene restores...
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The Bacillus amyloliquefaciens ribonuclease barnase (colored) and its inhibitor (blue) in a complex...
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Fersht LMB Profile Clarke, Jane (1993). Studies of disulphide mutants of barnase. jisc.ac.uk (PhD thesis). University of Cambridge. OCLC 53666398. EThOS uk...
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activity of its binding partner barnase, with which it forms an extraordinarily tightly bound complex within the cell until barnase is secreted. Expression of...
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BamHI restriction enzyme. It also synthesizes a natural antibiotic protein barnase, a widely studied ribonuclease that forms a famously tight complex with...
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Phi value analysis (section Example: barnase)
domains, WW domain, individual domains of proteins L and G, ubiquitin, and barnase have all been studied by ϕ {\displaystyle \phi } analysis. Phi is defined...
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Philosophy degree in 1993 for investigations of Bacterial Ribonuclease (Barnase) from the University of Cambridge supervised by Alan Fersht. Clarke was...
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Bacillus amyloliquefaciens is the source of a natural antibiotic protein barnase (a ribonuclease), alpha amylase used in starch hydrolysis, the protease...
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This may involve the use of a gene lethal to the host cells, such as barnase, Ccda, and the parD/parE toxins. This typically works by disrupting or...
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inhibitor proteins include the barstar inhibitor of the bacterial ribonuclease barnase. Animals and plants have evolved to synthesise a vast array of poisonous...
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resulted in the seminal application of phi-value analysis to the study of barnase, a bacterial RNAse used in many protein folding studies. Development of...
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bacterial gene barnase from transgenic plants. This gene kills any cell that expresses it and lacks barstar, the specific inhibitor of barnase activity. In...
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denatured RNA prior to sequencing. Similar to other ribonucleases such as barnase and RNase A, ribonuclease T1 has been popular for folding studies. Structurally...
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non-natural one. To remove the former, the plasmid is inserted into cells with a barnase gene (toxic) with a premature amber codon but without the non-natural amino...
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S2CID 2130725. Vu, ND; Feng, H; Bai, Y (30 Mar 2004). "The folding pathway of barnase: the rate-limiting transition state and a hidden intermediate under native...
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transcriptase 1993 – Beta helix of Pectate lyase 1994 – Collagen 1994 – Barnase/barstar complex 1994 – F1 ATPase 1995 – Heterotrimeric G proteins 1996 –...
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