Gene gun (redirect from Biolistic Particle Delivery System)
In genetic engineering, a gene gun or biolistic particle delivery system is a device used to deliver exogenous DNA (transgenes), RNA, or protein to cells...
18 KB (2,156 words) - 05:46, 30 October 2024
Biorobotics (section Biolistic method)
in genetic engineering: The plasmid method, the vector method and the biolistic method. This technique is used mainly for microorganisms such as bacteria...
18 KB (2,128 words) - 16:54, 23 October 2024
Gene delivery (section Biolistics)
membrane-repair mechanisms. Another method used to transform plant cells is biolistics, where particles of gold or tungsten are coated with DNA and then shot...
25 KB (2,878 words) - 19:30, 14 August 2024
insertion of genetic material into plant cells. Other methods include biolistics, where particles of gold or tungsten are coated with DNA and then shot...
134 KB (14,252 words) - 10:01, 5 November 2024
plasmids containing transgenes are inserted into the cells using the biolistic method or transkingdom bacterial conjugation (with 10−6 and 10−4 yield...
148 KB (15,296 words) - 18:57, 2 November 2024
the DNA is often inserted using Agrobacterium-mediated recombination, biolistics or electroporation. As only a single cell is transformed with genetic...
223 KB (24,619 words) - 13:41, 4 November 2024
chemical sensitisation of cells, electroporation, optical injection and biolistics. Finally, the transfected cells are cultured. As the aforementioned procedures...
108 KB (11,607 words) - 10:37, 14 November 2024
the DNA is often inserted using Agrobacterium-mediated recombination, biolistics or electroporation. As only a single cell is transformed with genetic...
144 KB (15,863 words) - 07:41, 2 November 2024
agricultural applications. Genes can be inserted into callus cells using biolistic bombardment, also known as a gene gun, or Agrobacterium tumefaciens. Cells...
17 KB (2,008 words) - 05:13, 19 February 2024
is agroinfiltration. Another method used to transform plant cells is biolistics, where particles of gold or tungsten are coated with DNA and then shot...
55 KB (6,765 words) - 09:23, 25 May 2024
micro-injection. Particle bombardment was made possible with the invention of the Biolistic Particle Delivery System (gene gun) by John Sanford in the 1980s. Transformation...
54 KB (6,762 words) - 07:39, 2 November 2024
the cell, namely: microinjection of nucleic acid with a fine needle; biolistic particle delivery, in which nucleic acid is attached to heavy metal particles...
34 KB (4,151 words) - 15:57, 2 November 2024
several pathways termed "agro-inoculation", including via rubbing, a biolistic delivery system, agrospray, agroinjection, and even via insect vectors...
48 KB (5,532 words) - 12:43, 9 October 2024
neurons in the developing visual cortex. During that time, she adapted biolistic transfection for use in transfecting neurons in organotypic slices. During...
10 KB (912 words) - 10:08, 24 October 2024
rice and wheat), wheat was the last to be transformed by transgenic, biolistic methods in 1992, and by Agrobacterium methods in 1997. Unlike corn and...
24 KB (2,384 words) - 01:40, 11 November 2024
Inserting DNA Agrobacteria Biolistics Electroporation Genetic transduction Lipofection Microinjection Somatic cell nuclear transfer Transfection Types...
9 KB (867 words) - 15:58, 10 October 2024
been transformed with both glass bead agitation and electroporation. The biolistic procedure appears to be the most efficient way of introducing DNA into...
34 KB (3,696 words) - 05:10, 2 November 2024
advantage of an electric current to pass foreign DNA through the cell wall; biolistics, which is the procedure of shooting DNA bullets into cells; and also delivering...
29 KB (3,583 words) - 18:09, 29 April 2024
isolating specific sequences necessary for replication. Gene gun delivery/Biolistics has been an attractive method for gene delivery due to its non-viral properties...
45 KB (5,772 words) - 17:07, 22 June 2024
genome of more complex cells and organisms. The gene gun, also known as a biolistic particle delivery system, is used to deliver transgenes, proteins, or...
11 KB (1,360 words) - 02:06, 3 December 2023
world (corn, rice and wheat), wheat was last transformed by transgenic, biolistic methods in 1992, and by Agrobacterium methods in 1997. Unlike maize and...
6 KB (714 words) - 18:27, 1 July 2024
and potential for cell or organelle damage (due to impact of biolistics). While biolistics and Agrobacterium are useful in specific species of plants-...
18 KB (2,592 words) - 18:58, 24 July 2023
more precise and convenient editing techniques. Gene guns (also known as biolistics) "shoot" (direct high energy particles or radiations against) target genes...
164 KB (16,854 words) - 07:41, 2 November 2024
employed: infectious bacterial-mediated (usually Agrobacterium) transfer and biolistics, with the latter being most commonly applied to allopolyploid cereals...
50 KB (5,497 words) - 22:56, 12 November 2024
inserted into a tomato cell's chloroplast and chromoplast plastomes using biolistics. Tomatoes were the first food crop with an edible fruit where this was...
31 KB (3,338 words) - 10:20, 25 May 2024
new organism. New genetic information can be inserted with gene guns/biolistics. An example of a gene gun transgenic is the rainbow papaya, which is modified...
14 KB (1,726 words) - 04:31, 26 January 2024
of the DNA into a host organism was accomplished with the invention of biolistics, Agrobacterium-mediated recombination and microinjection. The first genetically...
59 KB (6,419 words) - 05:18, 5 September 2024
transgenic worms is to inject exogenous DNA into the syncytial germ line; biolistic transformation can also be used. History of model organisms The C. elegans...
7 KB (887 words) - 22:07, 10 October 2024
Binomial expansion Binomial theorem Biochemical genetics Bioinformatics Biolistic Bioremediation Biotechnology Biparental zygote Birth defect Bivalent (genetics)...
33 KB (2,517 words) - 18:31, 3 September 2024
Inserting DNA Agrobacteria Biolistics Electroporation Genetic transduction Lipofection Microinjection Somatic cell nuclear transfer Transfection Types...
23 KB (3,268 words) - 16:33, 6 April 2024