COLD-PCR (co-amplification at lower denaturation temperature PCR) is a modified polymerase chain reaction (PCR) protocol that enriches variant alleles...
12 KB (1,607 words) - 10:43, 9 July 2024
Polymerase chain reaction (redirect from PCR oil)
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...
89 KB (11,135 words) - 11:52, 26 August 2024
real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article, RT-PCR will...
43 KB (5,536 words) - 07:35, 12 July 2024
Real-time polymerase chain reaction (redirect from Real-Time PCR)
(real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors...
43 KB (5,338 words) - 11:09, 12 July 2024
Thermal cycler (redirect from PCR machine)
thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). Thermal...
4 KB (493 words) - 09:01, 21 May 2024
(or colder) temperatures. Many variations and modifications of the PCR procedure have been developed in order to achieve higher yields; hot start PCR is...
22 KB (2,750 words) - 14:49, 30 April 2023
The versatility of polymerase chain reaction (PCR) has led to modifications of the basic protocol being used in a large number of variant techniques designed...
32 KB (4,102 words) - 05:25, 7 February 2024
Primer (molecular biology) (redirect from PCR-Primer)
laboratory for specific reactions such as polymerase chain reaction (PCR). When designing PCR primers, there are specific measures that must be taken into consideration...
16 KB (2,112 words) - 01:14, 11 April 2024
reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith and was awarded the Japan Prize in the same year. PCR became a...
47 KB (4,851 words) - 07:58, 30 August 2024
clinical significance of plasma-based KRAS mutation analysis using the COLD-PCR/TaqMan(®) -MGB probe genotyping method". Experimental and Therapeutic Medicine...
106 KB (11,809 words) - 15:18, 27 August 2024
History of polymerase chain reaction (redirect from History of PCR)
with the terms and components used in the PCR process.) The history of the polymerase chain reaction (PCR) has variously been described as a classic...
23 KB (3,101 words) - 10:26, 8 February 2024
Primer dimer (section Hot-start PCR)
primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of...
14 KB (1,573 words) - 10:45, 9 July 2024
Rapid amplification of cDNA ends (redirect from RACE-PCR)
to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. This technique is sometimes called one-sided PCR or anchored PCR. The first step in RACE...
8 KB (1,025 words) - 07:12, 11 March 2023
The Cold War originated in the breakdown of relations between the two main victors in World War II: United States and the Soviet Union, and their respective...
75 KB (9,644 words) - 05:52, 14 June 2024
COVID-19 testing (redirect from PCR testing procedure)
as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used. The Minimum...
181 KB (21,044 words) - 00:00, 2 September 2024
Genome Research (redirect from PCR Methods Appl.)
of interest to a broad readership. The journal was established in 1991 as PCR Methods and Applications and obtained its current title in 1995. According...
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modified PCR protocol that enriches variant alleles from a mixture of wild-type and mutation-containing DNA might be considered (e.g. COLD-PCR). The DNA...
26 KB (3,176 words) - 03:46, 22 January 2024
polymerase chain reaction (PCR) is employed to discover the lengths of the short tandem repeats based on the length of the PCR product. STR analysis is...
12 KB (1,458 words) - 09:06, 20 August 2024
keratoconjunctivitis is done using cell culture (with immunofluorescence staining) and PCR. It may appear similar to herpes simplex type I, Acanthamoeba, and fungal...
7 KB (674 words) - 23:28, 22 March 2024
DNA synthesis (section RT-PCR)
polymerase is combined with selective PCR amplification to produce many copies of mutant DNA. RT-PCR differs from conventional PCR as it synthesizes cDNA from mRNA...
14 KB (1,799 words) - 23:34, 8 April 2024
Microsatellite (section ISSR-PCR)
conventional PCR amplification and amplicon size determination. The use of PCR means that microsatellite length analysis is prone to PCR limitations like...
67 KB (7,393 words) - 17:16, 28 June 2024
components. The purified DNA can then be used for downstream applications such as PCR, sequencing, or cloning. Currently, it is a routine procedure in molecular...
24 KB (3,187 words) - 12:02, 9 July 2024
in the CNS, screening for VZV by PCR is recommended. Negative PCR does not rule out VZV involvement, but a positive PCR can be used for diagnosis, and appropriate...
79 KB (8,810 words) - 02:55, 30 August 2024
reaction (PCR) is type of NAAT which allows a very small sample of genetic material to be rapidly amplified into millions of copies for study. PCR is more...
98 KB (9,900 words) - 16:33, 21 July 2024
fidelity PCR enzymes, in: C.W. Dieffenbach, G.S. Dveksler (Eds.), PCR Primer: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor...
41 KB (5,164 words) - 23:57, 28 July 2024
Phylogenetic relationships of Sakhalin taimen Parahucho perryi inferred from PCR-RFLP analysis of mitochondrial DNA. Russian Journal of Genetics, 44:767....
3 KB (232 words) - 20:50, 11 August 2024
Chimera (molecular biology) (section PCR chimera)
artifact of PCR amplification. It occurs when the extension of an amplicon is aborted, and the aborted product functions as a primer in the next PCR cycle....
9 KB (971 words) - 18:35, 3 December 2023
symptoms of a viral infection, such as a cold. Otherwise, a rapid antigen detection test or throat swab is recommended. PCR testing has become common as it is...
26 KB (2,298 words) - 11:48, 31 July 2024
variations, like reverse transcription PCR (RT-PCR) for amplification of RNA, and, more recently, quantitative PCR which allow for quantitative measurement...
49 KB (5,528 words) - 13:46, 18 August 2024
diagnosis and therapy. Amoeba cultures and real-time polymerase chain reaction (PCR) studies for N. fowleri are diagnostic of PAM, but they are not readily available...
33 KB (3,535 words) - 00:24, 16 August 2024