COLD-PCR (co-amplification at lower denaturation temperature PCR) is a modified polymerase chain reaction (PCR) protocol that enriches variant alleles...

The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...

real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article, RT-PCR will...

(real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors...

thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). Thermal...

(or colder) temperatures. Many variations and modifications of the PCR procedure have been developed in order to achieve higher yields; hot start PCR is...

The versatility of polymerase chain reaction (PCR) has led to modifications of the basic protocol being used in a large number of variant techniques designed...

laboratory for specific reactions such as polymerase chain reaction (PCR). When designing PCR primers, there are specific measures that must be taken into consideration...

reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith and was awarded the Japan Prize in the same year. PCR became a...

clinical significance of plasma-based KRAS mutation analysis using the COLD-PCR/TaqMan(®) -MGB probe genotyping method". Experimental and Therapeutic Medicine...

with the terms and components used in the PCR process.) The history of the polymerase chain reaction (PCR) has variously been described as a classic...

primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of...

to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. This technique is sometimes called one-sided PCR or anchored PCR. The first step in RACE...

The Cold War originated in the breakdown of relations between the two main victors in World War II: United States and the Soviet Union, and their respective...

as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used. The Minimum...

of interest to a broad readership. The journal was established in 1991 as PCR Methods and Applications and obtained its current title in 1995. According...

modified PCR protocol that enriches variant alleles from a mixture of wild-type and mutation-containing DNA might be considered (e.g. COLD-PCR). The DNA...

polymerase chain reaction (PCR) is employed to discover the lengths of the short tandem repeats based on the length of the PCR product. STR analysis is...

keratoconjunctivitis is done using cell culture (with immunofluorescence staining) and PCR. It may appear similar to herpes simplex type I, Acanthamoeba, and fungal...

polymerase is combined with selective PCR amplification to produce many copies of mutant DNA. RT-PCR differs from conventional PCR as it synthesizes cDNA from mRNA...

conventional PCR amplification and amplicon size determination. The use of PCR means that microsatellite length analysis is prone to PCR limitations like...

components. The purified DNA can then be used for downstream applications such as PCR, sequencing, or cloning. Currently, it is a routine procedure in molecular...

in the CNS, screening for VZV by PCR is recommended. Negative PCR does not rule out VZV involvement, but a positive PCR can be used for diagnosis, and appropriate...

reaction (PCR) is type of NAAT which allows a very small sample of genetic material to be rapidly amplified into millions of copies for study. PCR is more...

fidelity PCR enzymes, in: C.W. Dieffenbach, G.S. Dveksler (Eds.), PCR Primer: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor...

Phylogenetic relationships of Sakhalin taimen Parahucho perryi inferred from PCR-RFLP analysis of mitochondrial DNA. Russian Journal of Genetics, 44:767....

artifact of PCR amplification. It occurs when the extension of an amplicon is aborted, and the aborted product functions as a primer in the next PCR cycle....

symptoms of a viral infection, such as a cold. Otherwise, a rapid antigen detection test or throat swab is recommended. PCR testing has become common as it is...

variations, like reverse transcription PCR (RT-PCR) for amplification of RNA, and, more recently, quantitative PCR which allow for quantitative measurement...

diagnosis and therapy. Amoeba cultures and real-time polymerase chain reaction (PCR) studies for N. fowleri are diagnostic of PAM, but they are not readily available...