In mass spectrometry, de novo peptide sequencing is the method in which a peptide amino acid sequence is determined from tandem mass spectrometry. Knowing...
29 KB (4,013 words) - 22:06, 29 July 2024
Protein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide. This may serve to identify...
21 KB (2,836 words) - 10:57, 8 February 2024
Protein mass spectrometry (redirect from De novo repeat detection)
have been described to identify peptides and proteins from tandem mass spectrometry (MS/MS), peptide de novo sequencing and sequence tag-based searching...
31 KB (3,815 words) - 23:50, 5 May 2024
sample. In contrast, the latter infers peptide sequences without knowledge of genomic data. De novo peptide sequencing algorithms are, in general, based on...
81 KB (3,974 words) - 15:56, 12 November 2024
PEAKS (category Proteomic sequencing)
peptide sequencing, protein identification and quantification. PEAKS is commonly used for peptide identification (Protein ID) through de novo peptide...
4 KB (371 words) - 09:59, 27 March 2024
iodoacetamide. It is commonly used during the sample preparation for de novo (peptide) sequencing with protein mass spectrometry, but recent critics suggest to...
5 KB (391 words) - 21:39, 17 April 2024
fragmentation pattern facilitates the applicability of these spectra for de novo peptide sequencing. Endoproteinase Lys-C Mass spectrometry Tandem mass spectrometry...
2 KB (188 words) - 02:17, 9 January 2023
and lipid transport proteins in the cell membranes of patients. De novo peptide sequencing Protein mass spectrometry Proteomics Mass spectrometry in the...
11 KB (1,420 words) - 03:11, 22 June 2024
Each extract may then be analysed, such as by peptide mass fingerprinting or de novo peptide sequencing after in-gel digestion. This can provide a great...
44 KB (5,065 words) - 05:47, 30 October 2024
peptide mass fingerprinting (PMF), if the identification is performed using the sequence data determined in tandem MS analysis it is called de novo peptide...
82 KB (10,326 words) - 02:15, 19 October 2024
Ancient protein (section De novo programmes)
associated with peptide spectral matches (PSMs). De novo sequencing is also possible for the analysis of ancient MS/MS spectra. It is a sequencing technique...
73 KB (7,903 words) - 19:06, 7 April 2024
characterization. Originally developed to identify novel peptides through de novo peptide sequencing, the technology has been adapted to address antibody...
7 KB (511 words) - 19:40, 13 September 2024
De novo gene birth is the process by which new genes evolve from non-coding DNA. De novo genes represent a subset of novel genes, and may be protein-coding...
128 KB (13,475 words) - 15:43, 28 October 2024
protein sample can be done in an ESI-MS by de novo peptide sequencing (using tandem mass spectrometry) or peptide mass fingerprinting. Both methods require...
40 KB (5,402 words) - 13:33, 11 September 2024
Scheuermann J, Leimbacher M, De Bellis G, Rizzi E, Dumelin C, Melkko S, Neri D (November 2008). "High-throughput sequencing allows the identification of...
42 KB (4,746 words) - 16:04, 11 November 2024
Human leukocyte antigen (section Gene sequencing)
class I (A, B, and C), all of which are the HLA Class1 group, present peptides from inside the cell. For example, if the cell is infected by a virus,...
51 KB (5,942 words) - 07:28, 11 November 2024
List of RNA-Seq bioinformatics tools (category RNA sequencing)
control tools for next generation sequencing (NGS) data, consisting of both raw reads quality evaluation and de novo contamination screening, which could...
164 KB (20,361 words) - 10:21, 2 November 2024
proteomics: The peptide sequence tag approach developed at the EMBL was one of the first methods for the identification of peptides based on mass spectra...
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Polyclonal antibodies (section Peptide antibodies)
Amber L.; Duan, Jin; Han, Xiaobing; Liu, Qixin; Ma, Bin (2024). "De novo protein sequencing of antibodies for identification of neutralizing antibodies in...
22 KB (2,626 words) - 21:37, 6 November 2024
Mervyn J. (30 June 2015). "The Streptomyces leeuwenhoekii genome: de novo sequencing and assembly in single contigs of the chromosome, circular plasmid...
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analogues include locked nucleic acids (LNA), morpholinos and peptide nucleic acids (PNA). In sequencing, dideoxynucleotides are used. These nucleotides possess...
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proline at position 6. Mass spectrometry is increasingly used for de novo sequencing of these hormones. AKH has become an important area of study, particularly...
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"Characterization of a peptide family from the skin secretion of the Middle East Tree Frog Hyla savignyi by composition-based de novo sequencing". Rapid Communications...
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particularly faster for data containing multiple smaller FASTA sequences, such as de-novo transcriptome assemblies. Coding region Putative gene Sequerome – A sequence...
16 KB (1,898 words) - 09:12, 17 March 2024
DNA Sequencing Is Here. Writing DNA Is Next". Wired. Retrieved 12 June 2017. Hwang, Samuel James (2008). DNA as a programmable material : de novo gene...
75 KB (7,525 words) - 03:31, 13 November 2024
bioinformatics, sequence analysis is the process of subjecting a DNA, RNA or peptide sequence to any of a wide range of analytical methods to understand its...
33 KB (3,894 words) - 07:15, 23 July 2024
out, removed the protein from the bead then the tethered peptide was identified by sequencing. A somewhat different approach was followed by Taylor and...
48 KB (6,512 words) - 12:40, 8 November 2024
when a fusion gene is expressed is called a fusion protein. Chimeric gene De novo gene birth ETV6-NTRK3 gene fusion Exon shuffling Gene duplication Horizontal...
17 KB (1,928 words) - 19:40, 5 August 2024
disease-causing, variants in the ASH1L gene. About half of the variants arise de novo, and half are inherited. Of the inherited variants, about half are maternally...
36 KB (3,070 words) - 20:04, 18 October 2024
Functional genomics (section RNA sequencing)
by genomic and transcriptomic projects (such as genome sequencing projects and RNA sequencing). Functional genomics focuses on the dynamic aspects such...
32 KB (4,013 words) - 04:45, 28 May 2024