A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with...

specific target protein. Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry and immunocytochemistry, where antibodies...

probes. A dot blot is a special case of any of the above blots where the analyte is added directly to the blotting matrix (and appears as a "dot") as opposed...

probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler dot blot assay. It is a common tool used in genetic testing...

affinity purification Protein:protein interaction studies Colony blot, dot blot, Western blot and ELISA Screening for positive expression clones Immunocytochemistry...

oligonucleotide (ASO) probes, a process that can often be done by a simple dot blot. Amplified fragment length polymorphism (AFLP) RAPD STR analysis Saiki...

antigen (with a test such as ELISA or antigen microarray assay) or immuno-dot blot. The most productive and stable clone is then selected for future use....

chemically modified paper filters or microporous membrane filters. In a dot blot, macromolecules are applied directly to the matrix. Macromolecules can...

functionally mapping epitopes often use binding assays such as western blot, dot blot, and/or ELISA to determine antibody binding. Competition methods look...

oligonucleotide (ASO) probes, a process that can often be done by a simple dot blot. RFLP https://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechCAPS.shtml...

antigen-antibody reactions are being explored, such as immuno-PCR. A rapid dot-blot ELISA test is also available for quick, effective, and economical on-site...

Enzymology vol. 155(F) pp. 335–50 (1987). Verlaan-de Vries M et al. "A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides...

rabbit antisera against representative strains with diverse origins and by Dot blot assays. These results endorsed the establishment of two different groups...

Allium L. subgenus Rhizirideum (G. Don ex Koch) Wendelbo according to dot blot hybridization with randomly amplified DNA probes". Theoretical and Applied...

analyzed, either a very specific antibody needs to be used in quantitative dot blot analysis (QDB), or biochemical separation then needs to be used before...

spectrometry (MS). However, a recent developed method of quantitative dot blot (QDB) analysis is able to measure both the absolute and relative quantity...

Railroader. On a calendar he would mark a dot for each hour worked, half a dot for a half hour, and a blot for anything thing that seemed like 3 hours...

stationary phase Reverse phase protein lysate microarray, a micro-cell lysate dot-blot that allows measurement of protein expression levels This disambiguation...

nylon membranes, which can be accomplished by use of dot blots or bidirectional agarose gel blotting and UV fixation of the DNA to the membranes. In many...

molecular diagnosis based in test samples. Some of these methods (like Dot-blot hybridisation) allow the scientists to detect the viroid even six months...

protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression levels in a large...

health screening study on 35,365 people, using a sensitive chemiluminescent dot blot assay". Sensors. 11 (12): 11064–80. Bibcode:2011Senso..1111064C. doi:10...

(1999). "Screening for hereditary fructose intolerance mutations by reverse dot-blot". Mol. Cell. Probes. 13 (1): 35–40. doi:10.1006/mcpr.1998.0208. PMID 10024431...

circovirus by using polymerase chain reaction (PCR)-based methods and dot blot hybridisation (DBH) tests. Infection with DuCV appears to cause growth...

DNA polymerase - DNA replication - DNA sequencing - DNase - dominant - dot blot - double helix - downstream (DNA) - downstream (transduction) - drimenol...

increased sensitivity compared to methods such as RNase protection assays and dot blot hybridization. The disadvantage to RT-PCR is the requirement of costly...

allele-specific diagnostic assay (MASDA). This program combines forward dot-blot, complex simultaneous probe hybridization and direct mutation detection...

Allium L. subgenus Rhizirideum (G. Don ex Koch) Wendelbo according to dot blot hybridization with randomly amplified DNA probes". Theoretical and Applied...

measured by the cccDNA levels via Southern blot kinetics of healthy vs infected cells and quantified by dot blot. In these infected cells, there is a strong...

identify avian reoviruses molecularly by observing infected tissues with dot-blot hybridization, PCR, and a combination of PCR and RFLP. This combination...