• Thumbnail for EcoRI
    EcoRI (pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices...
    8 KB (891 words) - 00:49, 14 June 2024
  • common and have greater biological importance than mirror-like palindromes. EcoRI digestion produces "sticky" ends, whereas SmaI restriction enzyme cleavage...
    55 KB (5,854 words) - 10:34, 24 June 2024
  • Thumbnail for Nuclease
    order in which the enzymes were isolated from single strains of bacteria: EcoRI, EcoRII. A restriction endonuclease functions by "scanning" the length of a...
    22 KB (2,535 words) - 04:02, 28 October 2023
  • Thumbnail for BioBrick
    of the plasmids is digested with EcoRI and SpeI. The plasmid carrying the other BioBrick part is digested with EcoRI and Xbal. This leaves both plasmids...
    22 KB (2,790 words) - 10:05, 26 August 2023
  • Thumbnail for Facioscapulohumeral muscular dystrophy
    in 10% of affected individuals. FSHD1 with a very large D4Z4 deletion (EcoRI 10-11 kb) is more strongly associated with infantile onset and severe weakness...
    174 KB (16,344 words) - 14:45, 13 July 2024
  • processes. Some examples of type II restriction endonucleases include BamHI, EcoRI, EcoRV, HindIII, and HaeIII. Type III, however, cleaves the DNA at about 25...
    25 KB (2,688 words) - 21:18, 6 April 2024
  • site for EcoRI, the commercially available pSC101 gained restriction sites for several enzymes, including HindIII, in addition to the EcoRI site. Manen...
    2 KB (228 words) - 16:08, 23 January 2024
  • of the plasmids is digested with EcoRI and SpeI while the second plasmid is digested with EcoRI and XbaI. The two EcoRI overhangs are complementary and...
    61 KB (7,073 words) - 16:39, 29 April 2024
  • to produce cohesive ends of DNA fragments. The commonly used linkers are EcoRI-linkers and sal-I linkers. Molecular Biology of The Cell, Fifth Edition...
    1 KB (191 words) - 10:43, 30 December 2022
  • Thumbnail for Golden Gate Cloning
    BsaI, BsmBI, and BbsI. Unlike standard Type II restriction enzymes like EcoRI and BamHI, these enzymes cut DNA outside of their recognition sites and...
    18 KB (2,489 words) - 05:22, 30 April 2024
  • activity was introduced by Mayer who characterized the modified activity in EcoRI. Star Activity - New England Biolabs Star Activity (Relaxation of Specificity)...
    3 KB (276 words) - 21:44, 24 May 2024
  • a series of mobility (mob) genes. Its size and the presence of a single EcoRI recognition site caused it to be considered as a vector candidate. ColE1...
    3 KB (392 words) - 16:13, 23 January 2024
  • recognition site, or somewhere nearby. For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the...
    5 KB (629 words) - 12:27, 17 March 2022
  • Thumbnail for EcoRV
    -sheet, and a long α-helix. This subdomain is found only in EcoRV and PvuII. Like EcoRI, EcoRV forms a homodimer in solution before binding and acting on...
    5 KB (506 words) - 00:49, 14 June 2024
  • Thumbnail for PBR322
    The circular sequence is numbered such that 0 is the middle of the unique EcoRI site and the count increases through the TetR gene. If we have to remove...
    13 KB (943 words) - 15:51, 13 March 2024
  • Thumbnail for Stanley Norman Cohen
    single site for the restriction enzyme EcoRI and a gene for tetracycline resistance. The restriction enzyme EcoRI was used to cut the frog DNA into small...
    26 KB (2,603 words) - 19:34, 23 January 2024
  • Thumbnail for Aquidneck Island
    "PFAS Contamination from Aquidneck Island Navy Base Worries Neighbors". ecoRI News. Retrieved 2022-08-05. Aquidneck Indian Council, "A Brief History of...
    12 KB (1,276 words) - 12:04, 4 July 2024
  • Thumbnail for Cre recombinase
    recombination system. EcoRI fragments of the P1 bacteriophage genome were generated and cloned into lambda vectors. A 6.5kb EcoRI fragment (Fragment 7)...
    15 KB (1,745 words) - 01:46, 14 June 2024
  • Thumbnail for Multiple cloning site
    polylinker). It has restriction sites for various restriction enzymes, including EcoRI, BamHI, and PstI. Another vector used in genetic engineering is pUC19, which...
    5 KB (683 words) - 08:04, 18 May 2024
  • Retrieved 2018-02-01. "Mass. Firm Works to Make Medical Waste Cleaner, Safer". ecoRI News. Retrieved 2018-02-01. Recycler, American. "American Recycler News...
    5 KB (231 words) - 12:03, 6 June 2023
  • Ronald W. Davis first discovered sticky ends as the product of the action of EcoRI, the restriction endonuclease. Sticky end links are different in their stability...
    8 KB (1,091 words) - 08:30, 22 February 2024
  • Thumbnail for Charlestown, Rhode Island
    (September 13, 2011). "Charlestown Strikes Up the Ban on Wind Turbines". ecoRI News. Retrieved September 24, 2017. "Census of Population and Housing"....
    15 KB (1,407 words) - 20:09, 2 November 2023
  • Thumbnail for CRISPR interference
    CRISPR-Gold - CRISPRa - CRISPRi Easi-CRISPR - FACE Enzyme Cas9 - FokI - EcoRI - PstI - SmaI HaeIII - Cas12a (Cpf1) - xCas9 Applications CAMERA - ICE -...
    24 KB (2,898 words) - 12:08, 22 March 2024
  • Thumbnail for Ligation (molecular biology)
    compatible ends and become ligated in a sticky-end (or cohesive end) ligation. EcoRI for example generates an AATT end, and since A and T have lower melting...
    38 KB (4,862 words) - 03:50, 15 May 2024
  • Thumbnail for CRISPR
    CRISPR-Gold - CRISPRa - CRISPRi Easi-CRISPR - FACE Enzyme Cas9 - FokI - EcoRI - PstI - SmaI HaeIII - Cas12a (Cpf1) - xCas9 Applications CAMERA - ICE -...
    152 KB (16,267 words) - 04:20, 10 July 2024
  • Thumbnail for PUC19
    sites for HindIII, SphI, PstI, SalI, XbaI, BamHI, SmaI, KpnI, SacI and EcoRI restriction enzymes have been derived from the vector M13mp19. This plasmid...
    7 KB (873 words) - 18:39, 1 July 2024
  • Thumbnail for Filling station
    (2015-05-20). "Rural Rhode Island Town at Epicenter of Leaking Fuel Tank Story". ecoRI News. Retrieved 2023-10-13. Jenkins, Robin R.; Guignet, Dennis; Walsh, Patrick...
    97 KB (10,285 words) - 19:03, 8 July 2024
  • Thumbnail for Plastic bag bans in the United States
    ecoRI News. Archived from the original on October 30, 2017. Retrieved October 31, 2017. "Middletown Bans the Bag; Could Portsmouth be Next?". ecoRI News...
    195 KB (7,997 words) - 06:37, 28 May 2024
  • Thumbnail for Cas12a
    CRISPR-Gold - CRISPRa - CRISPRi Easi-CRISPR - FACE Enzyme Cas9 - FokI - EcoRI - PstI - SmaI HaeIII - Cas12a (Cpf1) - xCas9 Applications CAMERA - ICE -...
    13 KB (1,530 words) - 12:46, 22 March 2024
  • Thumbnail for M13 bacteriophage
    no protein content. George Smith, among others, showed that fragments of EcoRI endonuclease could be fused in the unique Bam site of f1 filamentous phage...
    14 KB (1,714 words) - 23:57, 28 March 2024