The Klenow fragment is a large protein fragment produced when DNA polymerase I from E. coli is enzymatically cleaved by the protease subtilisin. First...

leave a useful molecule called the Klenow fragment, widely used in molecular biology. In fact, the Klenow fragment was used during the first protocols...

overhang (sticky ends) at the 3' end of the newly generated strand. The Klenow fragment of Bst (BF) has a strand displacement activity which allows for use...

polymerase that was in use before the discovery of Taq polymerase, the Klenow fragment (sourced from E. coli). Taq polymerase is well-suited for this application...

worked on the X-ray crystallographic structure of DNA polymerase I (Klenow Fragment) and its DNA complexes in collaboration with Dr. Cathy Joyce, Prof...

steps. The earliest thermal cyclers were designed for use with the Klenow fragment of DNA polymerase I. Since this enzyme is destroyed during each heating...

example, treatment of DNA polymerase I with subtilisin yields the Klenow fragment, which retains its polymerase function but lacks 5'-exonuclease activity...

overnight. This 5’ overhang provides the template required by the Klenow fragment of DNA Polymerase I to add biotinylated CTP or ATP to the digested...

using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication...

nuclease activity of this enzyme. The overall activity of the resulting Klenow fragment is therefore biased towards the synthesis of DNA, rather than its degradation...

enzyme. There are several DNA polymerases that are used in PCR. The Klenow fragment, derived from the original DNA Polymerase I from E. coli, was the first...

mismatched base pairs that have been missed by the proofreading element (Klenow fragment) of the DNA polymerase complex. The post-replicative Mismatch Repair...

RNA strand paired with a template strand. To begin synthesis, a short fragment of RNA, called a primer, must be created and paired with the template DNA...

Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment Eukaryotic (synthesis in S phase) Replication factor C RFC1 Flap endonuclease...

Kapler GM (December 2007). "Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition". The EMBO Journal. 26 (24):...

this protein displays a structural and functional similarity to the Klenow fragment of the Escherichia coli Polymerase I enzyme; it comprises a C-terminal...

reliable replication mechanism. Research into xDNA replication using a Klenow fragment from DNA polymerase I shows that a natural base partner is selectively...

ATPase - karyotype - KduI/IolB isomerase family - kilobase - kinase - Klenow fragment - Knock-down - knock-out - knock-out experiment - knockout - Kozak...

"A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides". Proceedings of the National Academy...

template DNA and are then extended by the Klenow fragment. Next, the templates are removed and the fragments are assembled by homology in a process similar...

Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment Eukaryotic (synthesis in S phase) Replication factor C RFC1 Flap endonuclease...

Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment Eukaryotic (synthesis in S phase) Replication factor C RFC1 Flap endonuclease...

oligonucleotide into a complete complementary DNA strand was the large (Klenow) fragment of E. coli DNA polymerase 1. However, ligation step can become an issue...

complementary oligonucleotide and by a subsequent Klenow fill-in DNA-polymerization, yielding a double stranded DNA fragment. The synthetic and encoding strategies...

Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment Eukaryotic (synthesis in S phase) Replication factor C RFC1 Flap endonuclease...

Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment Eukaryotic (synthesis in S phase) Replication factor C RFC1 Flap endonuclease...

Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment Eukaryotic (synthesis in S phase) Replication factor C RFC1 Flap endonuclease...

proteins that could be exported by fusion with MBP, include the monomeric Klenow polymerase and the dimeric Gene V protein of phage M13. When the recombinant...

purification of bifunctional hybrid proteins which bind maltose. Export of the Klenow polymerase into the periplasmic space". Eur J Biochem. 171 (3): 541–549...

1186/1743-422X-11-82. PMC 4018502. PMID 24885320. Winarski KL, Tang J, Klenow L, Lee J, Coyle EM, Manischewitz J, et al. (July 2019). "Antibody-dependent...