molecular biology, a protein-fragment complementation assay, or PCA, is a method for the identification and quantification of protein–protein interactions. In...
12 KB (1,265 words) - 08:24, 26 March 2024
Two-hybrid screening (category Protein–protein interaction assays)
protein-fragment complementation assay. Pioneered by Stanley Fields and Ok-Kyu Song in 1989, the technique was originally designed to detect protein–protein...
47 KB (6,112 words) - 03:07, 1 April 2024
Protein-fragment complementation assay Co-immunoprecipitation Affinity purification and mass spectrometry Proximity ligation assay Proximity labeling...
10 KB (1,069 words) - 18:26, 11 December 2023
transfer systems, mammalian protein–protein interaction trap, electro-switchable biosurfaces, protein–fragment complementation assay, as well as real-time label-free...
74 KB (8,396 words) - 00:09, 26 April 2024
atrophy, a form of dementia Prostate cancer Protein-fragment complementation assay, to identify protein–protein interactions Protocatechuic acid, a phenolic...
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pairs of proteins can be inferred from a variety of techniques, including yeast two-hybrid systems, protein-fragment complementation assays (PCA), affinity...
25 KB (2,915 words) - 08:44, 9 May 2024
Bimolecular fluorescence complementation (also known as BiFC) is a technology typically used to validate protein interactions. It is based on the association...
46 KB (5,192 words) - 21:00, 23 August 2022
mixture of proteins. Protein-fragment complementation assays are often used to detect protein–protein interactions. The yeast two-hybrid assay is the most...
26 KB (3,013 words) - 15:34, 4 August 2024
PFCA may refer to: Protein-fragment complementation assay Perfluorinated carboxylic acid The Planar Fourier Capture Array, a tiny camera built entirely...
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Antibody (redirect from Fragment variable)
rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection...
119 KB (13,400 words) - 17:29, 29 August 2024
Complement C2 is a protein that in humans is encoded by the C2 gene. The protein encoded by this gene is part of the classical pathway of the complement...
16 KB (2,068 words) - 18:45, 14 January 2024
SNP genotyping (redirect from Oligo Pool Assay)
performing a digestion on a genomic sample and determining fragment lengths through a gel assay it is possible to ascertain whether or not the enzymes cut...
37 KB (5,002 words) - 04:04, 22 June 2024
Dihydrofolate reductase (category Protein domains)
used as a tool to detect protein–protein interactions in a protein-fragment complementation assay (PCA), using a split-protein approach. DHFR-lacking CHO...
40 KB (4,581 words) - 15:52, 23 August 2024
signaling molecules, such as calcium or glutamate, protein phosphorylation state, protein complementation, receptor dimerization, and other processes provide...
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of complement (serum). Then cell death is determined with several approaches: Radioactive method: target cells are labeled with 51Cr before CDC assay, chromium...
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Respiratory syncytial virus (redirect from Respiratory syncytial virus F protein)
These include both enzyme immunosorbent assays (EIA) and chromatographic immunoassays (CIA). Direct fluorescence assay (DFA) allows for direct microscopic...
98 KB (9,900 words) - 16:33, 21 July 2024
X-gal (section Protein-protein interactions)
the principle of α-complementation of the β-galactosidase gene, where a fragment of the lacZ gene (lacZα) in the plasmid can complement another mutant lacZ...
11 KB (1,061 words) - 16:57, 16 October 2023
Complement component 4 (C4), in humans, is a protein involved in the intricate complement system, originating from the human leukocyte antigen (HLA) system...
35 KB (4,189 words) - 16:19, 7 November 2023
Molecular biology (section The Bradford protein assay)
accurate protein quantitation compared to previous methods: the Lowry procedure and the biuret assay. Unlike the previous methods, the Bradford assay is not...
49 KB (5,530 words) - 05:08, 5 September 2024
method is based on the principle of α-complementation of the β-galactosidase gene. This phenomenon of α-complementation was first demonstrated in work done...
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MM, Rüdiger SG (2012). "Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp". PLOS ONE. 7 (10): e46147. Bibcode:2012PLoSO...
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Proteolysis (redirect from Protein degradation)
Uversky, Vladimir N (ed.). "Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp". PLOS ONE. 7 (10): e46147. Bibcode:2012PLoSO...
34 KB (4,246 words) - 02:50, 14 August 2024
Proteomics (redirect from Protein analysis)
kinetic exclusion assay, and experimental methods such as phage display and in silico computational methods. Knowledge of protein-protein interactions is...
77 KB (9,012 words) - 20:46, 9 September 2024
Lipopolysaccharide (section Endotoxin Activity Assay)
biological assays, and chemical assays. Because the LPS is very difficult to measure in whole blood and because most LPS is bound to proteins and complement, the...
63 KB (7,188 words) - 21:59, 4 September 2024
Chemoproteomics assays can be stratified into three basic types. Solution-based approaches involve the use of drug analogs that chemically modify target proteins in...
63 KB (6,157 words) - 16:00, 30 March 2024
FANCA (redirect from Fanconi anemia, complementation group A)
gene. It belongs to the Fanconi anaemia complementation group (FANC) family of genes of which 12 complementation groups are currently recognized and is...
38 KB (4,760 words) - 00:26, 4 January 2024
CRISPR (redirect from CRISPR associated protein)
organisms such as bacteria and archaea. These sequences are derived from DNA fragments of bacteriophages that had previously infected the prokaryote. They are...
128 KB (13,448 words) - 03:26, 19 August 2024
Factor H (redirect from HF1 (protein))
Factor H (FH) is a member of the regulators of complement activation family and is a complement control protein. It is a large (155 kilodaltons), soluble glycoprotein...
29 KB (3,486 words) - 10:30, 30 August 2024
Transfer RNA (category Protein biosynthesis)
"Meta-analysis of tRNA derived RNA fragments reveals that they are evolutionarily conserved and associate with AGO proteins to recognize specific RNA targets"...
63 KB (7,572 words) - 16:45, 31 August 2024
Guideline on Mgen infections, in 2016, recommended complementing the molecular detection of Mgen with an assay capable of detecting macrolide resistance-associated...
45 KB (4,939 words) - 21:16, 22 July 2024