Southern blot is a method used for detection and quantification of a specific DNA sequence in DNA samples. This method is used in molecular biology. Briefly...
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name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. The major difference is that RNA, rather...
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electrophoresis prior to blotting. Southern blot for DNA northern blot for RNA reverse northern blot for RNA western blot for proteins far-western blot for protein–protein...
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widely known for the invention of the Southern blot, published in 1975 and now a common laboratory procedure. Southern was born in Burnley, Lancashire and...
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The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics...
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Molecular biology (section Southern blotting)
by Edwin Southern for the hybridisation of blotted DNA. Patricia Thomas, developer of the RNA blot which then became known as the northern blot, actually...
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matrix. Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography Northwestern Blot of Protein-RNA...
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The eastern blot, or eastern blotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids...
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as agarose gel electrophoresis and transferred to a membrane via the Southern blot procedure. Hybridization of the membrane to a labeled DNA probe then...
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for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying...
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underlying Southern blotting and Western blotting techniques of which they detect DNA and protein respectively. Similar to other types of blotting, proteins...
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paper and incubated with probes specific for speA. An image of this Southern blot can be seen in this article. Bacteriophages are very easily spread....
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a probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler dot blot assay. It is a common tool used in genetic testing...
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fluorescent protein vector Enzyme assay Protein purification Western blot Northern blot Southern blot Restriction enzyme Polymerase chain reaction Two-hybrid screening...
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range of biochemical methods, such as protein fingerprinting, Southern blot, other blotting procedures, DNA sequencing, and many more. Suspended particles...
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discernable fragments. Following the enzyme digestion, a Southern Blot is performed. Southern Blots are a size-based separation method that are performed...
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eukaryotic cells. One method is the Terminal Restriction Fragment (TRF) southern blot. There is a Web-based Analyser of the Length of Telomeres (WALTER),...
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D4Z4 array length, which is more precise and less labor-intensive than southern blot. Molecular combing is also available for assessing D4Z4 array length...
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A zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding, DNA sequences of different...
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chain reaction Northern blotting Southern blotting Fusion proteins DNA microarray Bioinformatics Flow cytometry Western blotting Chromatography ELISA X-ray...
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phosphorus with phosphorus-32. This technique is extensively used in Southern blot analysis of DNA samples. In this case, a phosphorus-32-containing DNA...
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Diagnostic tests include PCR to analyze the number of CGG repeats, Southern blot analysis, and examination of AGG trinucleotides in the FMR1 gene region...
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cannot be used to compare quantitative polymerase chain reaction and Southern blot telomere length measurement technique". International Journal of Epidemiology...
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ICSI. In molecular biology, PVP can be used as a blocking agent during Southern blot analysis as a component of Denhardt's buffer. It is also exceptionally...
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Shrapnel shell – Henry Shrapnel Sousaphone – John Philip Sousa Southern blot – Edwin Southern Soyer stove – Alexis Soyer Spragg Bag – Terry Spragg Sprengel...
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nucleic acid denaturation, the following methods were created: PCR Southern blot Northern blot DNA sequencing Acidic protein denaturants include: Acetic acid...
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disease diagnosis. In hereditary VHL disease, techniques such as the Southern blot and gene sequencing can be used to analyse DNA and identify mutations...
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for ribosomal RNAPages displaying wikidata descriptions as a fallback Southern blot – DNA analysis technique X-ray scattering techniques – family of non-destructive...
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higher. The most common tests for the detection of these diseases are: Southern blot to detect large deletions or duplications Polymerase chain reaction...
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such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization. Electrophoresis is a process that enables...
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