Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions...

bacteria two-hybrid system, conceived in 2000, which itself was inspired by the yeast one- and two-hybrid systems. Whereas the two-hybrid versions can assess...

ChIP-chip. Yeast one-hybrid System (Y1H) is used to identify which protein binds to a particular DNA fragment. Bacterial one-hybrid system (B1H) is used...

Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years...

Saccharomyces cerevisiae (/ˌsɛrəˈvɪsi.iː/) (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungal microorganisms). The species has...

between fungal species is common and well established, particularly in yeast. Yeast hybrids are widely found and used in human-related activities, such as brewing...

technique for studying the expression of genes. The system has two parts: the Gal4 gene, encoding the yeast transcription activator protein Gal4, and the UAS...

Marc; Walhout, Albertha J. M. (2004-10-15). "A Gateway-Compatible Yeast One-Hybrid System". Genome Research. 14 (10b): 2093–2101. doi:10.1101/gr.2445504...

industrial yeast strain Saccharomyces pastorianus is an interspecies hybrid of S. cerevisiae and the cold tolerant S. eubayanus. This hybrid is commonly...

of yeast, also known as yeast sexual reproduction, is a biological process that promotes genetic diversity and adaptation in yeast species. Yeast species...

dehydrogenase gene (GPD) [1]. This hybrid promoter was then utilized to express human immune interferon, a toxic substance to yeast that results in a reduced copy...

Candida albicans is an opportunistic pathogenic yeast that is a common member of the human gut flora. It can also survive outside the human body. It is...

studies in yeast, for example in yeast two-hybrid system for the study of protein-protein interaction. The vectors used in yeast two-hybrid system contain...

arrays. More recent efforts have utilized yeast one-hybrid systems, bacterial one-hybrid and two-hybrid systems, and mammalian cells. A promising new method...

overcoming by the emergence of yeast two-hybrid variants, such as the membrane yeast two-hybrid (MYTH) and the split-ubiquitin system, which are not limited to...

the extent that yeasts involved in beer and wine production commonly involve hybrids of so-called pure species. As such, the yeasts involved in what...

tac promoter/operator (dubbed PTAC) is one of the most widely used expression systems. Ptac is a strong hybrid promoter composed of the –35 region of...

protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system". Microbiology and Molecular Biology Reviews...

have been used on a large scale to map whole interactomes. The yeast two hybrid system (Y2H) is suited to explore the binary interactions among two proteins...

yeasts still used. The best-known region where spontaneous fermentation is still used is the Senne Valley in Belgium where lambic is produced. Hybrid...

the product of the yeast HIS3 gene (another IGPD), e.g. in the yeast two-hybrid system. As of late 2007, 3 structures have been solved for this class...

developed. More recent efforts have utilized yeast one-hybrid systems, bacterial one-hybrid and two-hybrid systems, and mammalian cells. A promising new method...

genome libraries may be screened for protein interaction using yeast or bacterial system. Where there is a successful interaction between proteins being...

in yeast two-hybrid screening to screen or to assay protein-protein interactions in eukaryotic cells from yeast to human. In the GAL4/UAS system, the...

Saccharomyces kudriavzevii, is a species of yeast in the Saccharomyces sensu stricto complex. Its type strain is NCYC 2889T. It is used in production...

aborted. Plant hybrids often suffer from an autoimmune syndrome known as hybrid necrosis. In the hybrids, specific gene products contributed by one of the parents...

analysis system Automatic quantification and scoring system Confirmation approaches Data analysis tools Tetrad (genetics) Yeast two-hybrid Synthetic...

many different reporter proteins and is also the basis for the yeast two-hybrid system, an archetypical PCA assay. Any protein that can be split into...

control of the strong TEF promoter from Ashbya gossypii. Mammalian cells, yeast, and other eukaryotes acquire resistance to geneticin (= G418, an aminoglycoside...

dimorphic fungus. Sporothrix schenckii can be found in one of two morphologies, hyphal or yeast. The hyphal form is found in the environment on plants...