In molecular biology and biotechnology, a fluorescent tag, also known as a fluorescent label or fluorescent probe, is a molecule that is attached chemically...

The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP...

recycled, marked by a green fluorescent tag, or may be sent to a lysosome for degradation, marked by a red fluorescent tag. The purpose of creating fusion...

native-PAGE gels with fluorescent probes bearing metal ions can be used for detection of a polyhistidine tagged protein. The HQ tag has alternating histidine...

probe labeling efficiency, the type of probe, and the type of dye. Fluorescently tagged antibodies or streptavidin are bound to the dye molecule. These secondary...

terminating bases has its own specific fluorescent tag. As the labelled DNA molecules are separated, the fluorescent label is excited by a UV source, and...

method to assess DNA and RNA concentration is to tag the sample with a Fluorescent tag, which is a fluorescent dye used to measure the intensity of the dyes...

further specifically and covalently tagged with a suitable ligand, such as a fluorescent dye. Since its introduction, SNAP-tag has found numerous applications...

selective binding to F-actin, derivatives of phalloidin containing fluorescent tags are used widely in microscopy to visualize F-actin in biomedical research...

the surface of an infected macrophage. The HIV virions have been marked with a green fluorescent tag and then viewed under a fluorescent microscope....

affinity tag) or can be chosen from five available fluorescent dyes including Coumarin, Oregon Green, Alexa Fluor 488, diAcFAM, and TMR. These fluorescent dyes...

include tagging with nucleotides containing radioactive phosphorus for radiolabelling, or using a primer labeled at the 5' end with a fluorescent dye. Dye-primer...

salt homidium chloride) is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques...

A computer determines what base was added by the wavelength of the fluorescent tag and records it for every spot on the chip. After each round, non-incorporated...

speed of fluorescent development and creating monomeric variants. Improved variants of RFP include mFruits (mCherry, mOrange, mRaspberry), mKO, TagRFP, mKate...

attached to one of four different fluorescent dyes. When a nucleotide is incorporated by the DNA polymerase, the fluorescent tag is cleaved off and diffuses...

Fluorescence tags are used to give visual readout on a protein. Green fluorescent protein (GFP) and its variants are the most commonly used fluorescence tags. More...

hinder the tagged molecule and affect the fluorescence polarity. Fluorophore molecules could be either utilized alone, or serve as a fluorescent motif of...

ligand binding by differential scanning fluorimetry of proteins tagged with green fluorescent protein (GFP). This technology is based on the principle that...

series of biochemical steps are then carried out. In the kth cycle, a fluorescent tag is introduced, the color of which corresponds to the identity of the...

Chemistry for their work with GFP. Man-made GFP became widely used as a fluorescent tag to show which cells or tissues express specific genes. The genetic...

two-dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells. This technique is very useful...

direct fluorescent antibody (DFA or dFA), also known as "direct immunofluorescence", is an antibody that has been tagged in a direct fluorescent antibody...

increased by labelling the substrates/products with a radioactive or fluorescent tag. Assay sensitivity has also been increased by switching protocols to...

detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, whereas CISH...

tissue or medium. Calcium imaging takes advantage of calcium indicators, fluorescent molecules that respond to the binding of Ca2+ ions by fluorescence properties...

qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark...

which are often derived from Aequorea victoria jellyfish. Fluorescent proteins are used to tag components in cells so that they can be studied using fluorescence...

are called fluorophores. Fluorescence imaging photographs fluorescent dyes and fluorescent proteins to mark molecular mechanisms and structures. It allows...

spores indicates that the sterilization process has not been met. Fluorescent-tagged strains, known as rapid-read BIs, are becoming more common to verify...