Electroporation, or electropermeabilization, is a technique in which an electrical field is applied to cells in order to increase the permeability of...
44 KB (5,320 words) - 05:36, 19 October 2024
Irreversible electroporation or IRE is a soft tissue ablation technique using short but strong electrical fields to create permanent and hence lethal...
74 KB (7,906 words) - 05:19, 25 October 2024
carried out using calcium phosphate (i.e. tricalcium phosphate), by electroporation, by cell squeezing, or by mixing a cationic lipid with the material...
34 KB (4,151 words) - 01:08, 21 November 2024
is now a routinely used laboratory procedure. Transformation using electroporation was developed in the late 1980s, increasing the efficiency of in-vitro...
54 KB (6,762 words) - 07:39, 2 November 2024
Transformation efficiency (section Electroporation)
transformation efficiency in electroporation, and inactivation of ligase or chloroform extraction of DNA may be necessary for electroporation, alternatively only...
23 KB (2,952 words) - 07:47, 19 May 2024
there is no carryover of the helper or donor strain. In contrast, electroporation does not require a helper or donor strain. This helps avoid possible...
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Lipid bilayer (section Electroporation)
this, electroporation is one of the key methods of transfection as well as bacterial transformation. It has even been proposed that electroporation resulting...
81 KB (9,795 words) - 01:24, 19 November 2024
Gene delivery (section Electroporation)
uses force to introduce genetic material through the cell membrane. Electroporation is a method of promoting competence. Cells are briefly shocked with...
25 KB (2,878 words) - 19:30, 14 August 2024
Entertainment Iron response element, a regulatory RNA sequence Irreversible electroporation, a medical soft tissue ablation method IRE (unit), a unit used to measure...
1 KB (206 words) - 01:53, 22 November 2022
Nucleofection is an electroporation-based transfection method which enables transfer of nucleic acids such as DNA and RNA into cells by applying a specific...
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Vectors in gene therapy (section Electroporation)
Electroporation is generally efficient and works across a broad range of cell types. However, a high rate of cell death following electroporation has...
36 KB (4,918 words) - 09:36, 27 February 2024
received his Ph.D. degree in chemical engineering in 1994, for a thesis “Electroporation of Tissue and Cells for Drug Delivery Applications”. At Georgia Tech...
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method that is chosen (e.g. transformation, transduction, transfection, electroporation). When microorganisms are able to take up and replicate DNA from their...
32 KB (4,016 words) - 17:56, 20 August 2024
achieved. In contrast to the thermal methods (extreme heat or cold) electroporation is being used and evaluated as a means of killing very small areas...
16 KB (1,944 words) - 16:08, 26 August 2024
Heterologous expression (section Electroporation)
This method has been successfully used both in vitro and in vivo. Electroporation is a method that uses high voltage to create pores in the membranes...
45 KB (5,772 words) - 17:07, 22 June 2024
particularly in terms of recovery time, is being evaluated. Irreversible electroporation is a relatively novel ablation technique with potential for downstaging...
122 KB (12,842 words) - 09:00, 11 November 2024
Modifying and Vascular-Disrupting Effects of Electroporation and Electrochemotherapy", Handbook of Electroporation, Cham: Springer International Publishing...
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An experiment or process performed in an egg or embryo (e.g. in ovo electroporation of chicken embryo). in pace ut sapiens aptarit idonea bello in peace...
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techniques are available, such as chemical sensitisation of cells, electroporation, optical injection and biolistics. Finally, the transfected cells are...
109 KB (11,790 words) - 21:58, 23 November 2024
polyurethane foam for the construction industry. It is also added after electroporation of yeasts in transformation protocols, allowing the cells to recover...
21 KB (2,058 words) - 14:38, 11 September 2024
pulsed (microsecond duration) electric fields to create irreversible electroporation in tissues. It is used most widely to treat tumors (cancer) or cardiac...
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fruit irradiated for fruit fly quarantine. Pulsed electric field (PEF) electroporation is a method for processing cells by means of brief pulses of a strong...
41 KB (5,009 words) - 06:30, 23 August 2024
can be easily followed (unlike mice). This also allows the use of electroporation for studying the effect of adding or silencing a gene. Other tools...
80 KB (9,216 words) - 23:31, 19 November 2024
Small interfering RNA (section Electroporation)
Cells". 13 October 2016. Takei Y (2014). "Electroporation-Mediated siRNA Delivery into Tumors". Electroporation Protocols. Methods in Molecular Biology...
56 KB (6,837 words) - 04:51, 29 October 2024
Chemistry Checkers Marshall nanoMOFs 2022 Biology Povilas Šimonis Electroporation of Yeast Cells 2020 Physics Jakub Kubečka Formation, Structure, and...
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Calorimeters Centrifuges Chromatography products Electrophoresis products Electroporation products Flame photometers Freeze dryers Histology products Refractometers...
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inserted using Agrobacterium-mediated recombination, biolistics or electroporation. As only a single cell is transformed with genetic material, the organism...
144 KB (15,863 words) - 05:40, 17 November 2024
be induced to take up foreign DNA, usually by exposed heat shock or electroporation. DNA is generally inserted into animal cells using microinjection,...
223 KB (24,627 words) - 12:34, 17 November 2024
Tissue nanotransfection (TNT) is an electroporation-based technique capable of gene and drug cargo delivery or transfection at the nanoscale. Furthermore...
17 KB (2,013 words) - 02:05, 6 May 2024
and oncogene cells by a chemical compound, mechanical therapy (e.g. electroporation), radiation, gene therapy, protein therapy, ultrasound waves, light...
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