A ligand binding assay (LBA) is an assay, or an analytic procedure, which relies on the binding of ligand molecules to receptors, antibodies or other...

In protein-ligand binding, the ligand is usually a molecule which produces a signal by binding to a site on a target protein. The binding typically results...

and Peter Perlmann in 1971. The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid...

effect in vivo. IC50 can be determined with functional assays or with competition binding assays. Sometimes, IC50 values are converted to the pIC50 scale...

intestine). tissue (e.g. limulus lysate) cell (e.g. platelets) Ligand binding assay when a ligand (usually a small molecule) binds a receptor (usually a large...

caused by the receptor or on ligand-receptor binding. Dose-response curves can be constructed to describe response or ligand-receptor complex formation...

effects on tissues. The development of the ligand binding assay in 1945 allowed quantification of the binding affinity of drugs at chemical targets. Modern...

attraction, an error in molecular phylogeny Ligand binding assay, an assay whose procedure relies on the binding of ligands to receptors, antibodies, and other...

alterations was radioligand binding thermostability assay. The assay is performed by incubating the protein with a radiolabelled ligand of the protein for 30...

dose-response relationships are organ bath preparations, ligand binding assays, functional assays, and clinical drug trials. Specific to response to doses...

Electrophoretic mobility shift assay (EMSA) is a widespread qualitative technique to study protein–DNA interactions of known DNA binding proteins. DNA-Protein-Interaction...

binding selectivity is defined with respect to the binding of ligands to a substrate forming a complex. Binding selectivity describes how a ligand may...

binding capabilities have higher volumes of distribution than drugs which are more polar, more highly ionized or exhibit high plasma protein binding in...

chromatography Electrophoresis Ligand binding assays Dual polarisation interferometry ELISA (Enzyme-linked immunosorbent assay) MIA (magnetic immunoassay)...

similar to ligand design (i.e., design of a molecule that will bind tightly to its target). Although design techniques for prediction of binding affinity...

radioactive isotope and the cell-targeting compound - the ligand. The ligand is the target binding site, it may be on the surface of the targeted cancer cell...

relationship Hill equation (biochemistry) Schild plot Del Castillo Katz model Cheng-Prussoff Equation Methods (Organ bath, Ligand binding assay, Patch clamp)...

antibody epitope binning, peptide synthesis, immunoassay development, ligand-binding assay analysis, and support for CAR-T research. Antibody Solutions was...

hybridization assay is a type of Ligand Binding Assay (LBA) used to quantify nucleic acids in biological matrices. Hybridization assays can be in solution...

to predict the binding-conformation of small molecule ligands to the appropriate target binding site. Characterisation of the binding behaviour plays...

The ABC transporters, ATP synthase (ATP)-binding cassette transporters are a transport system superfamily that is one of the largest and possibly one...

receptor antagonist is a type of receptor ligand or drug that blocks or dampens a biological response by binding to and blocking a receptor rather than activating...

can be detected during purification steps by a ligand binding assay, often using a radioactive ligand. In order to evaluate the process of multistep purification...

Ligand efficiency is a measurement of the binding energy per atom of a ligand to its binding partner, such as a receptor or enzyme. Ligand efficiency is...

lateral flow test (LFT), is an assay also known as a lateral flow device (LFD), lateral flow immunochromatographic assay, or rapid test. It is a simple...

been widely used in scientific research as a hot ligand in androgen receptor (AR) ligand binding assays (LBAs) and as a photoaffinity label for the AR....

specificity is the ability of binding site of a macromolecule (such as a protein) to bind specific ligands. The fewer ligands a protein can bind, the greater...

A kinetic exclusion assay (KinExA) is a type of bioassay in which a solution containing receptor, ligand, and receptor-ligand complex is briefly exposed...

of molecules with specific features like glycan content or ligand binding. Pull-down assays are a common variation of immunoprecipitation and immunoelectrophoresis...

allowing ligand access to the binding site. Divalent cations (e.g. Mg2+) are also required for integrin-ligand binding. Integrin ligands ICAM-1 and...