Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication...

being the first mammal cloned from a somatic cell. Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a...

"Foundations of Molecular Cloning - Past, Present and Future | NEB". www.neb.com. Retrieved 2021-11-25. "Foundations of Molecular Cloning - Past, Present...

Golden Gate Cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple...

Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after...

and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism...

and recombination, and is also an essential laboratory procedure in molecular cloning, whereby DNA fragments are joined to create recombinant DNA molecules...

In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence...

molecules formed by laboratory methods of genetic recombination (such as molecular cloning) that bring together genetic material from multiple sources, creating...

involved in the writing of the “Molecular Cloning Manual”. In 1979, the Maniatis lab had developed and deployed gene cloning methods. Maniatis and Ed Fritsch...

Topoisomerase-based cloning (TOPO cloning) is a molecular biology technique in which DNA fragments are cloned into specific vectors without the requirement...

and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a...

Cloning is the process of making an identical copy of something. Cloning may also refer to: Cloning (programming), the copying of a programming object...

associated cloning data to non-profit and academic laboratories around the world. Addgene provides a free online database of plasmid cloning information...

circular DNA molecules with a few genes on them. In the process known as molecular cloning, researchers can amplify the DNA fragments by inserting plasmids into...

editions of the best-selling, highly influential laboratory manual Molecular Cloning, the third in 2001 with David Russell and the fourth in 2012 with...

for DNA modification in laboratories, and they are a vital tool in molecular cloning. The term restriction enzyme originated from the studies of phage...

Hayashizaki Y, Takahashi Y, Himeno S, Matsubara K (December 1983). "Molecular cloning of the human gastrin gene". Nucleic Acids Research. 11 (23): 8197–8203...

length, biofilm formation and host colonization. Most strains used by molecular biologists are derivatives of E. coli K-12, and possess both Dam and Dcm...

Functional cloning is a molecular cloning technique that relies on prior knowledge of the encoded protein’s sequence or function for gene identification...

circumstances. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within...

Maniatis T, Fritsch EF, Sambrook J (1982). "Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. Vol. 1. p. 5.4. ISBN 978-0879691363. Stephen...

sequencing. Cloning Molecular cloning Polymerase chain reaction TA cloning Williams, Steven A.; et al. (2006). Laboratory Investigations in Molecular Biology...

1074/jbc.M307996200. PMID 14507919. Sambrook J, Russel DW (2001). Molecular Cloning: A Laboratory Manual 3rd Ed. Cold Spring Harbor Laboratory Press....

recombinant DNA techniques, such as restriction digests, ligations and molecular cloning. There are a number of techniques used to insert genetic material...

and propagated in a population of microbes through the process of molecular cloning. There are different types of DNA libraries, including cDNA libraries...

based cloning methods such as Gibson assembly, which overcome the limitations of traditional restriction enzyme digestion and ligation cloning methods...

can also be used to add new restriction sites to a multiple cloning site. Multiple cloning sites are a feature that allows for the insertion of foreign...

against separation. Barrere GC, Barber CE, Daniels MJ (December 1986). "Molecular cloning of genes involved in the production of the extracellular polysaccharide...

use in molecular biology laboratories for recombinant DNA experiments (see Research applications). Purified DNA ligase is used in gene cloning to join...