The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP...
63 KB (7,449 words) - 17:30, 13 November 2024
Fluorescent proteins include: Green fluorescent protein (GFP) Yellow fluorescent protein (YFP) Red fluorescent protein (RFP) This set index article includes...
457 bytes (55 words) - 21:36, 1 April 2021
Red fluorescent protein (RFP) is a protein which acts as a fluorophore, fluorescing red-orange when excited. The original variant occurs naturally in the...
5 KB (516 words) - 15:18, 19 November 2024
biology research in order to track protein interactions in real time. The first fluorescent tag, green fluorescent protein (GFP), was isolated from Aequorea...
19 KB (2,305 words) - 05:22, 15 May 2024
enzymatic labeling, protein labeling, and genetic labeling are widely utilized. Ethidium bromide, fluorescein and green fluorescent protein are common tags...
24 KB (2,782 words) - 17:34, 31 October 2024
Fluorophore (redirect from Fluorescent dye)
there are also much larger natural fluorophores that are proteins: green fluorescent protein (GFP) is 27 kDa, and several phycobiliproteins (PE, APC....
29 KB (2,272 words) - 22:09, 11 November 2024
Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation...
4 KB (430 words) - 16:52, 12 May 2024
Fluorescence (redirect from Fluorescent)
identified as a carrier of green fluorescent protein (GFP) by Osamu Shimomura. The gene for these green fluorescent proteins has been isolated and is scientifically...
99 KB (11,057 words) - 17:35, 28 December 2024
Roger Y. Tsien (section Fluorescent proteins)
in Chemistry in 2008 for his discovery and development of the green fluorescent protein, in collaboration with organic chemist Osamu Shimomura and neurobiologist...
42 KB (3,942 words) - 08:58, 21 December 2024
characteristics usually involve fluorescent and luminescent proteins. Examples include the gene that encodes jellyfish green fluorescent protein (GFP), which causes...
17 KB (1,981 words) - 02:30, 9 December 2024
protein-rich but relatively low in food energy. They are also used in cell and molecular biology research, especially the green fluorescent protein used...
119 KB (12,438 words) - 16:11, 9 November 2024
Fluorescence microscope (redirect from Fluorescent microscopy)
microscope. Single molecule detection of normal blinking fluorescent dyes like green fluorescent protein (GFP) can be achieved by using a further development...
25 KB (2,729 words) - 08:21, 10 November 2024
known as the source of aequorin (a photoprotein), and green fluorescent protein (GFP); two proteins involved in bioluminescence. Their discoverers, Osamu...
12 KB (1,462 words) - 02:28, 11 December 2024
green fluorescent protein structure was resolved, many other organisms have been shown to exhibit biofluorescence and many new fluorescent proteins have...
19 KB (1,983 words) - 21:44, 28 October 2024
Lancelet (section Fluorescent proteins)
monomeric green fluorescent protein known as mNeonGreen, which is the brightest known monomeric green or yellow fluorescent protein. Lancelets are passive...
63 KB (6,459 words) - 11:34, 28 December 2024
and Roger Y. Tsien "for the discovery and development of the green fluorescent protein, GFP". He holds a PhD in neurobiology from Harvard University...
13 KB (1,161 words) - 20:40, 25 September 2024
clone and sequence the genes for the photoprotein aequorin and green fluorescent protein (GFP) and for his proposal to use GFP as a tracer molecule. He...
19 KB (2,069 words) - 00:25, 17 October 2024
Immunofluorescence (redirect from Fluorescent antibody technique)
fluorescence, one can utilize recombinant proteins containing fluorescent protein domains, e.g., green fluorescent protein (GFP). The GFP-technique involves altering...
21 KB (2,054 words) - 16:47, 21 April 2024
Aequorin (category Protein methods)
the protein was isolated from the animal by Osamu Shimomura in 1962. In the animal, the protein occurs together with the green fluorescent protein to produce...
29 KB (3,171 words) - 06:07, 8 June 2024
gene that encodes the green fluorescent protein (GFP), originally extracted from a jellyfish, that naturally produced bright green fluorescence. They inserted...
22 KB (2,319 words) - 00:26, 11 December 2024
EosFP is a photoactivatable green to red fluorescent protein. Its green fluorescence (516 nm) switches to red (581 nm) upon UV irradiation of ~390 nm...
22 KB (2,618 words) - 00:03, 28 March 2024
Bimolecular fluorescence complementation (category Protein–protein interaction assays)
technology typically used to validate protein interactions. It is based on the association of fluorescent protein fragments that are attached to components...
46 KB (5,192 words) - 21:00, 23 August 2022
purification. Fluorescence tags are used to give visual readout on a protein. Green fluorescent protein (GFP) and its variants are the most commonly used fluorescence...
24 KB (2,683 words) - 01:39, 3 December 2024
phycoerythrin or green fluorescent protein). The intrinsic DNA fluorescence is very weak.Alternatively, specific or general proteins, nucleic acids, lipids...
29 KB (3,385 words) - 08:57, 21 December 2024
The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from...
6 KB (612 words) - 23:00, 23 October 2023
images can be compared. Finally, the green fluorescent protein method genetically engineers mice to express a protein in active neurons, and a camera can...
20 KB (2,633 words) - 22:21, 9 December 2024
example of this, green fluorescent protein (GFP), fluoresces green when exposed to light in the blue to UV range. Fluorescent proteins are excellent reporter...
15 KB (1,905 words) - 16:52, 25 September 2024
GCaMP (category Proteins)
developed in 2001 by Junichi Nakai. It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin...
28 KB (3,065 words) - 01:46, 30 March 2024
gene green fluorescent protein can be visualized by stimulating it with blue light and then using a digital camera to record green fluorescent emission...
12 KB (1,411 words) - 06:14, 28 October 2024
measuring pH inside of organelles, a technique utilizing pH-sensitive green fluorescent proteins (GFPs) may be used. Overall, all three methods have their own...
17 KB (1,895 words) - 12:23, 24 December 2024