field of molecular biology. Ligation in the laboratory is normally performed using T4 DNA ligase. It is broadly used in vitro as molecular biology research...

Directionality, in molecular biology and biochemistry, is the end-to-end chemical orientation of a single strand of nucleic acid. In a single strand of...

Look up ligation in Wiktionary, the free dictionary. Ligation may refer to: Ligation (molecular biology), the covalent linking of two ends of DNA or RNA...

solely RNA primers, while laboratory techniques in biochemistry and molecular biology that require in vitro DNA synthesis (such as DNA sequencing and polymerase...

In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence...

Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication...

cellular and molecular biology is a list of definitions of terms and concepts commonly used in the study of cell biology, molecular biology, and related...

In Molecular biology, an insert is a piece of DNA that is inserted into a larger DNA vector by a recombinant DNA technique, such as ligation or recombination...

Multiplex ligation-dependent probe amplification (MLPA) is a variation of the multiplex polymerase chain reaction that permits amplification of multiple...

Dawson PE (March 2019). "Native Chemical Ligation of Peptides and Proteins". Current Protocols in Chemical Biology. 11 (1): e61. doi:10.1002/cpch.61. PMC 6384150...

In molecular biology, mutagenesis is an important laboratory technique whereby DNA mutations are deliberately engineered to produce libraries of mutant...

blunt ends, but blunt-end ligation is inefficient and requires a higher concentration of both DNA and DNA ligase than the ligation of sticky ends does. For...

biology have emerged from studies of RNA (ribonucleic acid), including seminal work in the fields of biochemistry, genetics, microbiology, molecular biology...

In molecular biology, subcloning is a technique used to move a particular DNA sequence from a parent vector to a destination vector. Subcloning is not...

Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see Research applications)...

preparation with SAFE Hi-C uses ligation products for deep sequencing to improve traditional Hi-C analysis". Communications Biology. 2 (1): 267. doi:10.1038/s42003-019-0519-y...

In biochemistry, a ligase is an enzyme that can catalyze the joining (ligation) of two molecules by forming a new chemical bond. This is typically via...

"Lecture 20: Gel Electrophoresis". BCH5425 Molecular Biology and Biotechnology. Bowen, R (20 October 1999). "DNA Ligation". Biotechnology and Genetic Engineering...

sequence of DNA—a phenomenon Francis Crick called the central dogma of molecular biology. The specific sequence of amino acids results in a unique three-dimensional...

Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order to...

vector) grow into blue colonies. Molecular cloning is one of the most commonly used procedures in molecular biology. A gene of interest may be inserted...

in plants List of geneticists Human Genome Project Dot plot (bioinformatics) Multiplex Ligation-dependent Probe Amplification Sequence analysis v t e...

single assay. The design of the molecular inversion probes (MIP) originated from padlock probes, a molecular biology technique first reported by Nilsson...

KP (2012). "Palindromic sequence impedes sequencing-by-ligation mechanism". BMC Systems Biology. 6 (Suppl 2): S10. doi:10.1186/1752-0509-6-S2-S10. PMC 3521181...

NdeI is an endonuclease isolated from Neisseria denitrificans. In molecular biology, it is commonly used as a restriction enzyme. Recognition sequence...

Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras". Next Generation Sequencing. Methods in Molecular Biology. Vol. 1712. pp. 71–85...

was the first enzyme isolated from this strain.[citation needed] In molecular biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky...

segment (called an intein) is removed from a precursor protein with a ligation of C-terminal and N-terminal external proteins (called exteins) on both...

exonuclease and annealing step while the other is for DNA polymerase and ligation steps. For the two-step approach, different incubation temperatures are...

Molecular Biology. 148: 31–8. Zaychikov E, Schickor P, Denissova L, and Heumann H. (2001) Hydroxyl radical footprinting. Methods in Molecular Biology...