In biochemistry, a nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds...
22 KB (2,535 words) - 04:02, 28 October 2023
Genome editing (redirect from Genome editing with engineered nucleases)
current nuclease-based gene editing platforms but its use was limited by low efficiencies of editing. Genome editing with engineered nucleases, i.e. all...
78 KB (9,362 words) - 13:30, 27 July 2024
Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses...
7 KB (842 words) - 06:09, 29 November 2023
Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal...
5 KB (536 words) - 14:58, 26 August 2023
Chimeric nucleases are an example of engineered proteins which must comprise a DNA-binding domain to give sequence specificity and a nuclease domain for...
2 KB (229 words) - 19:29, 29 August 2023
Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made...
27 KB (3,147 words) - 04:36, 16 May 2024
Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger...
36 KB (4,657 words) - 08:51, 23 July 2024
Surveyor nuclease assay is an enzyme mismatch cleavage assay used to detect single base mismatches or small insertions or deletions (indels). Surveyor...
26 KB (3,176 words) - 03:46, 22 January 2024
MNase-seq (redirect from Micrococcal nuclease sequencing)
MNase-seq, short for micrococcal nuclease digestion with deep sequencing, is a molecular biological technique that was first pioneered in 2006 to measure...
39 KB (4,420 words) - 19:10, 15 May 2024
Nuclease protection assay is a laboratory technique used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample...
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FokI (redirect from Fok1 nuclease)
helices α4 and α5 and two loops P1 and P2 of the cleavage domain. When the nuclease is unbound to DNA, the endonuclease domain is sequestered by the DNA-binding...
5 KB (628 words) - 19:09, 2 December 2023
Crab duplex-specific nuclease is a nuclease derived from the red king crab (Paralithodes camtschaticus, Kamchatka crab) hepatopancreas that displays a...
2 KB (178 words) - 20:57, 9 July 2024
Mung bean nuclease (Nuclease MB) is a nuclease derived from sprouts of the mung bean (Vigna radiata) that removes nucleotides in a step-wise manner from...
5 KB (718 words) - 20:54, 18 December 2022
CUT&RUN sequencing, also known as cleavage under targets and release using nuclease, is a method used to analyze protein interactions with DNA. CUT&RUN sequencing...
16 KB (2,049 words) - 03:28, 3 December 2023
unintended genetic modifications that can arise through the use of engineered nuclease technologies such as: clustered, regularly interspaced, short palindromic...
53 KB (6,846 words) - 19:05, 8 July 2024
enzymes known as "exo-endonucleases", however, are not limited to either nuclease function, displaying qualities that are both endo- and exo-like. Evidence...
25 KB (2,688 words) - 21:18, 6 April 2024
DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system. The PAM is a component...
12 KB (1,421 words) - 00:22, 14 December 2022
Serratia marcescens nuclease (EC 3.1.30.2, endonuclease (Serratia marcescens), barley nuclease, plant nuclease I, nucleate endonuclease) is an enzyme....
13 KB (1,147 words) - 09:21, 30 August 2024
Ribonuclease V1 (redirect from Nuclease V1)
retroviruses, such as hepatitis C, dengue virus, and HIV. Together with S1 nuclease, which specifically cleaves single-stranded RNA, it can be used to profile...
7 KB (707 words) - 05:59, 27 January 2024
APEX1 (redirect from APEX nuclease 1)
Information, U.S. National Library of Medicine. "Entrez Gene: APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1". Parker A, Gu Y, Mahoney W, Lee...
11 KB (1,319 words) - 18:41, 13 August 2023
Cas9 contains two nuclease domains: an McrA-like HNH nuclease domain and a RuvC-like nuclease domain. These HNH and RuvC-like nuclease domains are responsible...
43 KB (5,610 words) - 21:17, 2 May 2024
Prime editing (section Nuclease Prime Editor)
response, increasing the efficiency of prime editing. Nuclease Prime Editor uses Cas9 nuclease instead of Cas9(H840A) nickase. Unlike prime editor 3 (PE3)...
26 KB (3,050 words) - 04:06, 9 August 2024
with the dual ligation assay. The nuclease hybridization assay, also called S1 nuclease cutting assay, is a nuclease protection assay-based hybridization...
11 KB (1,345 words) - 11:17, 17 March 2022
CRISPR-associated systems, cas 1–4. The Cas proteins showed helicase and nuclease motifs, suggesting a role in the dynamic structure of the CRISPR loci....
128 KB (13,448 words) - 03:26, 19 August 2024
Cas nuclease or single effector protein will cause interference guided by the crRNA match. Type-I CRISPR systems are characterized by Cas3, a nuclease-helicase...
10 KB (1,149 words) - 06:02, 17 June 2024
SNP genotyping (section 5’- nuclease)
2007).. A broad range of enzymes including DNA ligase, DNA polymerase and nucleases have been employed to generate high-fidelity SNP genotyping methods. Restriction...
37 KB (5,002 words) - 04:04, 22 June 2024
ERCC4 (section Structure-specific nuclease)
enzyme complex that participates in DNA repair and DNA recombination. The nuclease enzyme ERCC1-XPF cuts specific structures of DNA. Many aspects of these...
27 KB (3,315 words) - 00:33, 27 January 2024
Zinc finger (section Zinc finger nucleases)
for a specific sequence is an area of active research, and zinc finger nucleases and zinc finger transcription factors are two of the most important applications...
42 KB (4,829 words) - 16:45, 1 August 2024
(the nuclease Cas9) and (b) the DNA sequences of previously encountered viral invaders (CRISPR). These viral DNA sequences enable the nuclease to target...
155 KB (14,572 words) - 15:53, 31 August 2024
DNA (section Nucleases and ligases)
are most accessible. Nucleases are enzymes that cut DNA strands by catalyzing the hydrolysis of the phosphodiester bonds. Nucleases that hydrolyse nucleotides...
166 KB (17,865 words) - 10:19, 31 August 2024