A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase...
43 KB (5,338 words) - 11:09, 12 July 2024
Reverse transcription polymerase chain reaction (redirect from Reverse Transcriptase quantitative PCR)
technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article...
43 KB (5,536 words) - 07:35, 12 July 2024
real time PCR with the use of allele specific probes. insertions and deletions are analyzed by dosage measurements using quantitative real time PCR.[citation...
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Digital polymerase chain reaction (redirect from Digital PCR)
PCR inhibitors or primer template mismatch. Real-time Digital PCR (rdPCR) combines the methodologies of digital PCR (dPCR) and quantitative PCR (qPCR)...
69 KB (7,347 words) - 05:12, 9 September 2024
Polymerase chain reaction (redirect from PCR oil)
qPCR is the appropriate contractions for quantitative PCR (real-time PCR). Reverse Complement PCR (RC-PCR): Allows the addition of functional domains...
89 KB (11,135 words) - 11:52, 26 August 2024
A quantitative PCR instrument is a machine that amplifies and detects DNA. It combines the functions of a thermal cycler and a fluorimeter, enabling the...
10 KB (1,008 words) - 19:36, 1 May 2024
Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments...
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the U.S. are performing screening for SCID in newborns using real-time quantitative PCR to measure the concentration of T-cell receptor excision circles...
34 KB (3,270 words) - 01:33, 14 August 2024
hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher Kary Mullis at...
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Bisulfite sequencing (redirect from Methylation specific PCR)
"Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection". Nucleic...
34 KB (4,084 words) - 21:59, 8 January 2024
Jean-Marc; La Scola, Bernard; Raoult, Didier (2012). "Comparison of real-time quantitative PCR and culture for the diagnosis of emerging Rickettsioses". PLOS...
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Thermal cycler (redirect from PCR machine)
and cold zones on a microfluidic chip. Thermal cyclers designed for quantitative PCR have optical systems which enable fluorescence to be monitored during...
4 KB (493 words) - 09:01, 21 May 2024
Rocchi; Michele Zappella & Alessandra Renieri (August 2004). "Real-time quantitative PCR as a routine method for screening large rearrangements in Rett...
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distance between the primer and the probe in some forms of real-time quantitative PCR (qPCR). The error rates of various polymerases (fidelity) have been...
41 KB (5,164 words) - 23:57, 28 July 2024
"Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis". Proceedings of the National...
3 KB (334 words) - 20:53, 13 September 2023
Multiplex polymerase chain reaction (redirect from Multiplex-PCR)
PMC 2642996. PMID 19240792. Abbs, S; Bobrow, M (1992). "Analysis of quantitative PCR for the diagnosis of deletion and duplication carriers in the dystrophin...
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Potato virus Y (section Quantitative PCR)
advantage of quantitative PCR is that analysis of resulting products using gel electrophoresis is not required. This means that quantitative PCR can be implemented...
38 KB (5,658 words) - 10:31, 7 September 2024
Hematopoietic Necrosis Virus and White Spot Virus in Shrimp Using Real-Time Quantitative PCR and SYBR Green Chemistry". Journal of Clinical Microbiology. 39...
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product during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods...
32 KB (4,102 words) - 05:25, 7 February 2024
County in 2000, Viracor was among the first to commercially offer real-time quantitative PCR assays to diagnose patients with Adenovirus, BK virus and JC virus...
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Resolution Melting (dHRM) is also used in conjunction with digital PCR (dPCR) to improve quantitative power by providing additional information on the melting behavior...
12 KB (1,448 words) - 07:05, 11 March 2024
McAlexander MA, Queen SE, Adams RJ (July 2013). "Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little...
307 KB (32,799 words) - 02:52, 4 September 2024
COVID-19 testing (redirect from PCR testing procedure)
described as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used....
181 KB (21,008 words) - 00:00, 2 September 2024
gene expression screening on 52 carcinoma samples and series of real-time quantitative PCR validation on 19 paired carcinomas and normal tissue samples and...
8 KB (1,008 words) - 01:13, 3 January 2024
previously limited to tracing individual transcripts by Northern blots or quantitative PCR. Higher throughput and speed allow researchers to frequently characterize...
31 KB (3,625 words) - 02:55, 30 July 2024
expression studies, such as microarray analysis, Northern blots, or quantitative real-time PCR (qPCR). RNA that has been degraded has a direct impact on calculated...
17 KB (2,067 words) - 01:32, 3 December 2023
S2CID 261516095. Liu Q, Allam B, Collier JL (July 2009). "Quantitative real-time PCR assay for QPX (Thraustochytriidae), a parasite of the hard clam...
5 KB (434 words) - 19:34, 7 May 2024
Harkness, W; Hayward, R; Thompson, D; Jacques, TS; et al. (2008). "Real-time quantitative PCR analysis of pediatric ependymomas identifies novel candidate genes...
47 KB (4,967 words) - 17:39, 20 November 2023
CM.; DeWoody, J. (2011). "The quantification of spermatozoa by real-time quantitative PCR, spectrophotometry, and spermatophore cap size". Molecular Ecology...
35 KB (4,203 words) - 08:16, 19 February 2024
transfected. mRNA levels of survivin were analyzed by real-time quantitative PCR (RT-PCR) and showed time-dependent down regulation of survivin mRNA levels...
62 KB (8,387 words) - 20:48, 11 August 2024