• In genetics, shotgun sequencing is a method used for sequencing random DNA strands. It is named by analogy with the rapidly expanding, quasi-random shot...
    23 KB (2,848 words) - 22:06, 25 July 2024
  • Thumbnail for RNA-Seq
    RNA-Seq (named as an abbreviation of RNA sequencing) is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules...
    103 KB (11,098 words) - 04:11, 29 July 2024
  • Thumbnail for Metagenomics
    scale and detail than before. Recent studies use either "shotgun" or PCR directed sequencing to get largely unbiased samples of all genes from all the...
    88 KB (9,198 words) - 10:06, 2 June 2024
  • Thumbnail for Genomics
    sequencing approaches fall into two broad categories, shotgun and high-throughput (or next-generation) sequencing. Shotgun sequencing is a sequencing...
    78 KB (7,545 words) - 14:27, 4 August 2024
  • directed sequencing, shotgun sequencing of DNA is a more rapid sequencing strategy. There is a technique from the "old time" of genome sequencing. The underlying...
    9 KB (1,168 words) - 21:29, 11 July 2023
  • Thumbnail for Whole genome sequencing
    genome sequencing of the fetus. Sequencing of nearly an entire human genome was first accomplished in 2000 partly through the use of shotgun sequencing technology...
    113 KB (9,499 words) - 05:21, 31 July 2024
  • Thumbnail for DNA sequencing
    published use of whole-genome shotgun sequencing, eliminating the need for initial mapping efforts. By 2001, shotgun sequencing methods had been used to produce...
    129 KB (14,415 words) - 11:32, 5 August 2024
  • Thumbnail for Human Genome Project
    project. Celera used a technique called whole genome shotgun sequencing, employing pairwise end sequencing, which had been used to sequence bacterial genomes...
    66 KB (7,381 words) - 15:38, 9 August 2024
  • The main ones are (1) massively parallel shotgun sequencing (MPSS), (2) targeted massive parallel sequencing (t-MPS) and (3) single nucleotide polymorphism...
    51 KB (5,952 words) - 19:05, 6 May 2024
  • in computer science Shotgun house, a type of narrow, rectangular house Shotgun sequencing, a method of sequencing DNA Shotgunning (cold reading), a "mind-reading"...
    3 KB (382 words) - 23:33, 13 May 2024
  • for shotgun sequencing or the study of complete genes in functional analysis. One major use of genomic libraries is hierarchichal shotgun sequencing, which...
    27 KB (3,403 words) - 18:03, 25 May 2024
  • is derived from shotgun sequencing of DNA which is itself named after the rapidly expanding, quasi-random firing pattern of a shotgun. The most common...
    15 KB (1,660 words) - 06:01, 12 January 2024
  • Thumbnail for Bioinformatics
    to be assembled to obtain complete gene or genome sequences. The shotgun sequencing technique (used by The Institute for Genomic Research (TIGR) to sequence...
    132 KB (8,414 words) - 01:03, 17 August 2024
  • Thumbnail for Prenatal testing
    gene-specific assay. Fetal cell-free DNA has been directly sequenced using shotgun sequencing technology. In one study, DNA was obtained from the blood plasma of...
    87 KB (8,888 words) - 20:45, 14 August 2024
  • Thumbnail for Whole genome bisulfite sequencing
    bisulfite sequencing was derived as a combination of this bisulfite treatment and next-generation sequencing technology, such as shotgun sequencing. The whole...
    22 KB (2,517 words) - 05:07, 4 July 2024
  • Thumbnail for Hybrid genome assembly
    various sequencing technologies to achieve the task of assembling a genome from fragmented, sequenced DNA resulting from shotgun sequencing. Genome assembly...
    21 KB (2,951 words) - 23:35, 28 July 2024
  • Sequence assembly (category DNA sequencing methods)
    technology used. Typically, the short fragments (reads) result from shotgun sequencing genomic DNA, or gene transcript (ESTs). The problem of sequence assembly...
    24 KB (2,864 words) - 15:34, 19 July 2024
  • faster and less laborious sequencing methods like whole genome shotgun sequencing and now more recently next-gen sequencing. repE for plasmid replication...
    8 KB (936 words) - 17:53, 18 June 2024
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    Remington, K; Heidelberg, JF; et al. (April 2004). "Environmental genome shotgun sequencing of the Sargasso Sea". Science. 304 (5667): 66–74. Bibcode:2004Sci...
    24 KB (2,585 words) - 21:15, 3 August 2024
  • Thumbnail for Microbiota
    but this applies to 16S rRNA amplicon sequencing as well and is a fundamental problem. With shotgun sequencing, it can be resolved by having a high coverage...
    69 KB (7,869 words) - 04:11, 29 July 2024
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    shotgun sequencing project". 13 March 2015. {{cite journal}}: Cite journal requires |journal= (help) "Picea glauca, whole genome shotgun sequencing project"...
    53 KB (5,510 words) - 13:20, 7 May 2024
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    genome was released. This version combined sequences gained from shotgun sequencing with the BAC-based sequence assemblies, which has helped to fill in...
    14 KB (1,061 words) - 07:35, 14 August 2023
  • Thumbnail for Genome project
    representation of the original chromosomes from which the DNA originated. In a shotgun sequencing project, all the DNA from a source (usually a single organism, anything...
    18 KB (1,822 words) - 00:58, 11 August 2024
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    response can surprisingly be much different. Sequencing technologies such as 16S rRNA shotgun metagenomic sequencing have facilitated the rapid expansion of...
    34 KB (3,730 words) - 14:54, 12 July 2024
  • Other than transcriptome shotgun sequencing, this technique has been called RNA-seq and whole transcriptome shotgun sequencing (WTSS). Once the cDNA fragments...
    30 KB (3,222 words) - 00:22, 10 February 2024
  • Thumbnail for Craig Venter
    genomics to transform healthcare radically. Venter believed that shotgun sequencing was the fastest and most effective way to get useful human genome...
    66 KB (5,965 words) - 12:03, 25 June 2024
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    November 2012). "Analysis of the bread wheat genome using whole-genome shotgun sequencing". Nature. 491 (7426): 705–710. Bibcode:2012Natur.491..705B. doi:10...
    95 KB (8,045 words) - 11:03, 11 August 2024
  • available to the public from GenBank. Celera's approach, which used shotgun sequencing, spurred the public HGP to accelerate its effort and shift its projected...
    15 KB (711 words) - 16:41, 11 August 2024
  • Thumbnail for Environmental DNA
    "Uncovering Trophic Interactions in Arthropod Predators through DNA Shotgun-Sequencing of Gut Contents". PLOS ONE. 11 (9): e0161841. Bibcode:2016PLoSO..1161841P...
    131 KB (13,420 words) - 08:03, 16 June 2024
  • Thumbnail for Gene mapping
    recombination. Genome sequencing is sometimes mistakenly referred to as "genome mapping" by non-biologists. The process of shotgun sequencing resembles the process...
    26 KB (3,363 words) - 22:19, 4 August 2024