• Thumbnail for Elution
    In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange...
    6 KB (658 words) - 09:51, 8 July 2024
  • Thumbnail for High-performance liquid chromatography
    constant ("isocratic elution mode") or varied ("gradient elution mode") during the chromatographic analysis. Isocratic elution is typically effective...
    89 KB (11,003 words) - 15:32, 20 September 2024
  • Thumbnail for Antibody elution
    An antibody elution is a clinical laboratory diagnostic procedure which removes sensitized antibodies from red blood cells, in order to determine the...
    12 KB (1,398 words) - 22:58, 14 September 2023
  • desorbed by an elution buffer. The mode used for elution (expanded-bed versus settled-bed) depends on the characteristics of the feed. After elution, the adsorbent...
    58 KB (7,255 words) - 18:24, 18 September 2024
  • Thumbnail for Size-exclusion chromatography
    gel (stationary phase). To determine approximate molecular weight, the elution volumes of compounds with their corresponding molecular weights are obtained...
    31 KB (3,897 words) - 09:56, 13 September 2024
  • Thumbnail for Ion chromatography
    molecules, thus separate them. This type of elution is called gradient elution. On the other hand, step elution can be used, in which the concentration of...
    54 KB (6,991 words) - 02:26, 21 July 2024
  • pre-incubated drugs should mirror the elution profiles of the targets they complex with. Correlation of drug and protein elution profiles allows for targets to...
    63 KB (6,157 words) - 16:00, 30 March 2024
  • elutes very late in the elution sequence, after fermium. Another method to isolate mendelevium exploits the distinct elution properties of Md2+ from those...
    33 KB (4,186 words) - 20:47, 25 August 2024
  • bound. Target biomolecules may then be removed by applying a so-called elution buffer, which disrupts interactions between the bound target biomolecules...
    29 KB (3,430 words) - 03:30, 24 June 2024
  • who in 1943 first classified the modes of chromatography as frontal, elution, and displacement. Displacement chromatography found a variety of applications...
    19 KB (2,639 words) - 18:13, 15 December 2023
  • Thumbnail for His-tag
    histidine and is typically used at a concentration of 150 - 500 mM for elution. Histidine or histamine can also be used. Decrease in pH When the pH decreases...
    22 KB (2,655 words) - 07:35, 10 August 2024
  • Thumbnail for Systematic evolution of ligands by exponential enrichment
    prepare for subsequent rounds of selection in which the stringency of the elution conditions can be increased to identify the tightest-binding sequences...
    41 KB (4,747 words) - 20:55, 12 September 2024
  • Thumbnail for Monoclonal antibody
    retained while impurities are washed away. An elution with a low pH buffer or a more gentle, high salt elution buffer is then used to recover purified antibody...
    48 KB (4,964 words) - 23:05, 30 September 2024
  • Thumbnail for Van Deemter equation
    the result of the first application of rate theory to the chromatography elution process. The van Deemter equation relates height equivalent to a theoretical...
    6 KB (968 words) - 08:02, 13 May 2024
  • Thumbnail for Thin-layer chromatography
    divided into solvent selectivity groups. Using solvents with different elution strengths or different selectivity groups can often give very different...
    23 KB (2,540 words) - 14:35, 25 September 2024
  • Thumbnail for Spin column-based nucleic acid purification
    particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in...
    5 KB (639 words) - 04:32, 4 March 2024
  • Thumbnail for Column chromatography
    chromatogram. The separate curves in the diagram represent different sample elution concentration profiles over time based on their affinity to the column...
    18 KB (2,378 words) - 05:27, 10 July 2024
  • Thumbnail for Tartrazine
    wool and silks, a colorant in food, drugs and cosmetics and an adsorption-elution indicator for chloride estimations in biochemistry. Tartrazine was discovered...
    27 KB (2,775 words) - 15:42, 30 September 2024
  • Elution: chromatographic separation of Fm(100), Es(99), Cf, Bk, Cm and Am...
    32 KB (4,022 words) - 06:00, 17 September 2024
  • known (see Mark–Houwink equation), a plot of log [η]M versus elution volume (or elution time) for a particular solvent, column and instrument provides...
    19 KB (2,347 words) - 02:09, 20 July 2024
  • divalent state, unlike the other trivalent actinides: under typically used elution conditions (bis-(2-ethylhexyl) phosphoric acid (HDEHP) as stationary organic...
    39 KB (8,151 words) - 20:49, 25 August 2024
  • isolated from samples of its parent 227Ac, the francium being milked via elution with NH4Cl–CrO3 from an actinium-containing cation exchanger and purified...
    36 KB (4,059 words) - 01:17, 19 August 2024
  • Thumbnail for Countercurrent chromatography
    partition coefficient from the partitioning experiment will indicate the elution behavior of the compound. Typically, it is desirable to choose a solvent...
    61 KB (7,354 words) - 14:33, 14 August 2024
  • binding of biotinylated DNA to streptavidin-coated magnetic beads and elution of single DNA strands selectively using alkali. The method allowed robotic...
    1 KB (163 words) - 03:44, 27 December 2023
  • Thumbnail for Muscimol
    in water, and passed down a column of cellulose phosphate. A subsequent elution with ammonium hydroxide and recrystallization from alcohol results in pure...
    37 KB (3,825 words) - 04:22, 8 September 2024
  • these materials and are afterwards desorbed either thermally (Tenax) or by elution (DNPH) and then analyzed by GC–MS/FID or HPLC. Reference gas mixtures are...
    60 KB (6,519 words) - 04:22, 2 October 2024
  • Thumbnail for Gallium
    as a pharmaceutical is chemical, and the radionuclide is extracted by elution from germanium-68, a synthetic radioisotope of germanium, in gallium-68...
    74 KB (8,761 words) - 07:52, 20 September 2024
  • Thumbnail for Rare-earth element
    X-ray fluorescence (XRF). Before the time that ion exchange methods and elution were available, the separation of the rare earths was primarily achieved...
    154 KB (16,461 words) - 01:54, 19 September 2024
  • Thumbnail for Technetium-99m generator
    within two daughter half-lives. Hence, removing the daughter nuclide (elution process) from the generator ("milking" the cow) is reasonably done as often...
    12 KB (1,313 words) - 23:57, 21 September 2024
  • Thumbnail for Solid-phase extraction
    incomplete recovery of the analytes by SPE caused by incomplete extraction or elution. In the case of an incomplete extraction, the analytes do not have enough...
    13 KB (1,672 words) - 13:57, 10 January 2024