In biochemistry, a nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds...
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Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal...
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Genome editing (redirect from Genome editing with engineered nucleases)
current nuclease-based gene editing platforms but its use was limited by low efficiencies of editing. Genome editing with engineered nucleases, i.e. all...
78 KB (9,361 words) - 01:20, 25 September 2024
Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses...
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Nuclease protection assay is a laboratory technique used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample...
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Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger...
36 KB (4,665 words) - 00:56, 23 September 2024
Chimeric nucleases are an example of engineered proteins which must comprise a DNA-binding domain to give sequence specificity and a nuclease domain for...
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Surveyor nuclease assay is an enzyme mismatch cleavage assay used to detect single base mismatches or small insertions or deletions (indels). Surveyor...
26 KB (3,176 words) - 03:46, 22 January 2024
Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made...
27 KB (3,147 words) - 04:36, 16 May 2024
Serratia marcescens nuclease (EC 3.1.30.2, endonuclease (Serratia marcescens), barley nuclease, plant nuclease I, nucleate endonuclease) is an enzyme....
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MNase-seq (redirect from Micrococcal nuclease sequencing)
MNase-seq, short for micrococcal nuclease digestion with deep sequencing, is a molecular biological technique that was first pioneered in 2006 to measure...
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FokI (redirect from Fok1 nuclease)
helices α4 and α5 and two loops P1 and P2 of the cleavage domain. When the nuclease is unbound to DNA, the endonuclease domain is sequestered by the DNA-binding...
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Crab duplex-specific nuclease is a nuclease derived from the red king crab (Paralithodes camtschaticus, Kamchatka crab) hepatopancreas that displays a...
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Mung bean nuclease (Nuclease MB) is a nuclease derived from sprouts of the mung bean (Vigna radiata) that removes nucleotides in a step-wise manner from...
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DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system. The PAM is a component...
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Zinc finger (section Zinc finger nucleases)
for a specific sequence is an area of active research, and zinc finger nucleases and zinc finger transcription factors are two of the most important applications...
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foods. Cellulases, pectinases Clarify fruit juices. Molecular biology Nucleases, DNA ligase and polymerases Use restriction digestion and the polymerase...
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DNA (section Nucleases and ligases)
are most accessible. Nucleases are enzymes that cut DNA strands by catalyzing the hydrolysis of the phosphodiester bonds. Nucleases that hydrolyse nucleotides...
166 KB (17,854 words) - 13:16, 19 September 2024
enzymes known as "exo-endonucleases", however, are not limited to either nuclease function, displaying qualities that are both endo- and exo-like. Evidence...
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Restriction enzyme (redirect from Restriction nuclease)
be generated by fusing a natural or engineered DNA-binding domain to a nuclease domain (often the cleavage domain of the type IIS restriction enzyme FokI)...
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finger nucleases. Since 2009 more accurate and easier systems to implement have been developed. Transcription activator-like effector nucleases (TALENs)...
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APEX1 (redirect from APEX nuclease 1)
Information, U.S. National Library of Medicine. "Entrez Gene: APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1". Parker A, Gu Y, Mahoney W, Lee...
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CRISPR-associated systems, cas 1–4. The Cas proteins showed helicase and nuclease motifs, suggesting a role in the dynamic structure of the CRISPR loci....
128 KB (13,448 words) - 03:26, 19 August 2024
CUT&RUN sequencing, also known as cleavage under targets and release using nuclease, is a method used to analyze protein interactions with DNA. CUT&RUN sequencing...
16 KB (2,049 words) - 03:28, 3 December 2023
a result another nuclease must be recruited to cleave the flap. This second nuclease is DNA2 nuclease, which has a helicase-nuclease activity, that cleaves...
16 KB (2,112 words) - 01:32, 8 September 2024
SNP genotyping (section 5’- nuclease)
2007).. A broad range of enzymes including DNA ligase, DNA polymerase and nucleases have been employed to generate high-fidelity SNP genotyping methods. Restriction...
37 KB (5,002 words) - 04:04, 22 June 2024
Ribonuclease V1 (redirect from Nuclease V1)
retroviruses, such as hepatitis C, dengue virus, and HIV. Together with S1 nuclease, which specifically cleaves single-stranded RNA, it can be used to profile...
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carbohydrates such as starch and sugars into simple sugars such as glucose, and nucleases split nucleic acids into nucleotides. Digestive enzymes are found throughout...
18 KB (2,239 words) - 09:27, 1 August 2024
four families of engineered nucleases: meganucleases, zinc finger nucleases, transcription activator-like effector nucleases (TALENs), and the Cas9-guideRNA...
134 KB (14,248 words) - 23:17, 15 September 2024
(the nuclease Cas9) and (b) the DNA sequences of previously encountered viral invaders (CRISPR). These viral DNA sequences enable the nuclease to target...
155 KB (14,569 words) - 14:58, 24 September 2024