• Thumbnail for Nucleic acid test
    loop-mediated isothermal amplification (LAMP). Virtually all nucleic acid amplification methods and detection technologies use the specificity of Watson-Crick...
    11 KB (1,417 words) - 20:39, 20 July 2024
  • Nucleic acid sequence-based amplification, commonly referred to as NASBA, is a method in molecular biology which is used to produce multiple copies of...
    13 KB (1,634 words) - 11:47, 2 November 2024
  • Thumbnail for Nucleic acid sequence
    A nucleic acid sequence is a succession of bases within the nucleotides forming alleles within a DNA (using GACT) or RNA (GACU) molecule. This succession...
    22 KB (2,409 words) - 03:21, 27 November 2024
  • Thumbnail for Polymerase chain reaction
    contain humic acids may inhibit PCR amplification and lead to inaccurate results.[citation needed] Allele-specific PCR or The amplification refractory mutation...
    89 KB (11,135 words) - 14:28, 3 January 2025
  • diseases. LAMP is an isothermal nucleic acid amplification technique. In contrast to the polymerase chain reaction (PCR) technology, in which the reaction is...
    27 KB (3,191 words) - 17:59, 4 December 2024
  • Thumbnail for Peptide nucleic acid
    Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA. Synthetic peptide nucleic acid oligomers have been used in recent...
    15 KB (1,832 words) - 00:26, 22 October 2024
  • Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature...
    24 KB (2,923 words) - 19:52, 31 October 2024
  • Thumbnail for Transcription-mediated amplification
    Transcription-mediated amplification (TMA) is an isothermal (performed at constant temperature), single-tube nucleic acid amplification system utilizing two...
    2 KB (292 words) - 19:57, 2 November 2024
  • of the target DNA itself, and the amplification of the signal is linear, in contrast to the exponential amplification of the target DNA in PCR. CPT uses...
    8 KB (938 words) - 20:51, 3 December 2023
  • denaturation of double stranded DNA thereby including isothermal nucleic acid amplification. In vivo, DNA is replicated by DNA polymerases with various accessory...
    6 KB (741 words) - 21:52, 19 October 2024
  • material for amplification can be used to divide these molecular methods into three groups: Target amplification which uses the nucleic acid itself. Just...
    9 KB (1,138 words) - 01:04, 4 March 2024
  • Thumbnail for Tuberculous meningitis
    1155/2012/831292. PMC 3359676. PMID 22666577. "CDC |TB | ...the Uses of Nucleic Acid Amplification Tests for the Diagnosis of TB: Background". www.cdc.gov. Archived...
    14 KB (1,286 words) - 14:14, 24 August 2024
  • Threose nucleic acid (TNA) is an artificial genetic polymer in which the natural five-carbon ribose sugar found in RNA has been replaced by an unnatural...
    10 KB (1,216 words) - 21:06, 26 December 2024
  • Thumbnail for Reverse Transcription Loop-mediated Isothermal Amplification
    Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a one step nucleic acid amplification method to multiply specific sequences of RNA...
    15 KB (1,765 words) - 01:04, 18 June 2024
  • be used. One way to classify tests is by whether it is a nucleic acid test or non-nucleic acid test. Variation in cost and the time it takes to get a result...
    31 KB (3,065 words) - 13:35, 14 October 2024
  • Thumbnail for Amplified fragment length polymorphism
    (11 November 1995). "AFLP: a new technique for DNA fingerprinting". Nucleic Acids Research. 23 (21): 4407–4414. doi:10.1093/nar/23.21.4407. ISSN 0305-1048...
    7 KB (754 words) - 03:50, 30 December 2024
  • Thumbnail for DNA sequencing
    process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the...
    130 KB (14,899 words) - 08:39, 27 December 2024
  • Solid-phase reversible immobilization (category Nucleic acids)
    of purifying nucleic acids from solution. It uses silica- or carboxyl-coated paramagnetic beads, which reversibly bind to nucleic acids in the presence...
    3 KB (355 words) - 16:17, 10 December 2024
  • targets to determine relative abundance of nucleic acid sequences in the target. The original nucleic acid arrays were macro arrays approximately 9 cm...
    54 KB (5,400 words) - 06:08, 28 October 2024
  • performing amplification of nucleic acid with two primers bound to a single solid support", published 1997-06-24, assigned to Mosaic Technologies Inc. and...
    30 KB (3,056 words) - 12:04, 2 December 2024
  • small DNA or RNA fragments generated directly from the target nucleic acid. The amplification products can be detected by a variety of standard methods,...
    5 KB (400 words) - 02:17, 7 January 2024
  • Thumbnail for Thermostable DNA polymerase
    David Edward Birch, Walter Joseph Laird, Michael Anthony Zoccoli: Nucleic acid amplification using a reversibly inactivated thermostable enzyme. United States...
    41 KB (5,164 words) - 18:26, 29 November 2024
  • Thumbnail for GelRed
    GelRed is an intercalating nucleic acid stain used in molecular genetics for agarose gel DNA electrophoresis. GelRed structurally consists of two ethidium...
    4 KB (223 words) - 11:00, 28 December 2024
  • (October 2000). "Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms". Nucleic Acids Res. 28 (20): 87e–87. doi:10...
    2 KB (300 words) - 17:15, 8 June 2024
  • quantify nucleic acids by amplifying a nucleic acid molecule with the enzyme DNA polymerase. Conventional PCR is based on the theory that amplification is exponential...
    69 KB (7,351 words) - 04:46, 30 October 2024
  • amplification include whole genome amplification (WGA), Nucleic acid sequence-based amplification (NASBA), and transcription-mediated amplification (TMA)...
    32 KB (4,102 words) - 05:25, 7 February 2024
  • be enough to ensure that non-specific amplification does not occur. Another chemical modification of nucleic acid is through the heat-reversible covalent...
    22 KB (2,750 words) - 14:49, 30 April 2023
  • loop-mediated isothermal amplification (LAMP) assay for research use. This assay can be used to test for the presence of virus through nucleic acid detection, returning...
    23 KB (2,213 words) - 20:53, 17 September 2024
  • through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and, if long enough...
    8 KB (1,025 words) - 07:12, 11 March 2023
  • Thumbnail for Aminoallyl nucleotide
    Aminoallyl nucleotide (category Nucleic acids)
    aminoallyl labeling is NASBA ( Nucleic Acid Sequence Based Amplification), a highly sensitive technique for amplifying RNA. In this specific case, the...
    13 KB (1,413 words) - 15:01, 8 January 2024