Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions...

bacteria two-hybrid system, conceived in 2000, which itself was inspired by the yeast one- and two-hybrid systems. Whereas the two-hybrid versions can assess...

Polge C, Lentze N, Auerbach D, Schlattner U (2009). "Yeast two-hybrid, a powerful tool for systems biology". International Journal of Molecular Sciences...

ChIP-chip. Yeast one-hybrid System (Y1H) is used to identify which protein binds to a particular DNA fragment. Bacterial one-hybrid system (B1H) is used...

Saccharomyces cerevisiae (/ˌsɛrəˈvɪsi.iː/) (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungal microorganisms). The species has...

industrial yeast strain Saccharomyces pastorianus is an interspecies hybrid of S. cerevisiae and the cold tolerant S. eubayanus. This hybrid is commonly...

Marc; Walhout, Albertha J. M. (2004-10-15). "A Gateway-Compatible Yeast One-Hybrid System". Genome Research. 14 (10b): 2093–2101. doi:10.1101/gr.2445504...

between fungal species is common and well established, particularly in yeast. Yeast hybrids are widely found and used in human-related activities, such as brewing...

mating of yeast, also known as yeast sexual reproduction, is a fundamental biological process that promotes genetic diversity and adaptation in yeast species...

Candida albicans is an opportunistic pathogenic yeast that is a common member of the human gut flora. It can also survive outside the human body. It is...

overcoming by the emergence of yeast two-hybrid variants, such as the membrane yeast two-hybrid (MYTH) and the split-ubiquitin system, which are not limited to...

studies in yeast, for example in yeast two-hybrid system for the study of protein-protein interaction. The vectors used in yeast two-hybrid system contain...

dehydrogenase gene (GPD) [1]. This hybrid promoter was then utilized to express human immune interferon, a toxic substance to yeast that results in a reduced copy...

technique for studying the expression of genes. The system has two parts: the Gal4 gene, encoding the yeast transcription activator protein Gal4, and the UAS...

arrays. More recent efforts have utilized yeast one-hybrid systems, bacterial one-hybrid and two-hybrid systems, and mammalian cells. A promising new method...

tac promoter/operator (dubbed PTAC) is one of the most widely used expression systems. Ptac is a strong hybrid promoter composed of the –35 region of...

developed. More recent efforts have utilized yeast one-hybrid systems, bacterial one-hybrid and two-hybrid systems, and mammalian cells. A promising new method...

have been used on a large scale to map whole interactomes. The yeast two hybrid system (Y2H) is suited to explore the binary interactions among two proteins...

yeasts still used. The best-known region where spontaneous fermentation is still used is the Senne Valley in Belgium where lambic is produced. Hybrid...

the extent that yeasts involved in beer and wine production commonly involve hybrids of so-called pure species. As such, the yeasts involved in what...

genome libraries may be screened for protein interaction using yeast or bacterial system. Where there is a successful interaction between proteins being...

Saccharomyces kudriavzevii, is a species of yeast in the Saccharomyces sensu stricto complex. Its type strain is NCYC 2889T. It is used in production...

protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system". Microbiology and Molecular Biology Reviews...

in yeast two-hybrid screening to screen or to assay protein-protein interactions in eukaryotic cells from yeast to human. In the GAL4/UAS system, the...

analysis system Automatic quantification and scoring system Confirmation approaches Data analysis tools Tetrad (genetics) Yeast two-hybrid Synthetic...

ubiquitin-like conjugation system. This complex binds and activates Atg3, which covalently attaches mammalian homologues of the ubiquitin-like yeast protein ATG8 (LC3A-C...

the product of the yeast HIS3 gene (another IGPD), e.g. in the yeast two-hybrid system. As of late 2007, 3 structures have been solved for this class...

aborted. Plant hybrids often suffer from an autoimmune syndrome known as hybrid necrosis. In the hybrids, specific gene products contributed by one of the parents...

zebra/ostrich hybrid, a giant panda/bat hybrid, a hippopotamus/gorilla hybrid, a platypus/sheep hybrid, a tiger/elephant hybrid, a rhinoceros/kangaroo hybrid, a...

2008). "Reconstruction of the genome origins and evolution of the hybrid lager yeast Saccharomyces pastorianus". Genome Research. 18 (10): 1610–1623. doi:10...