Two-hybrid screening (originally known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions...

most conventional and widely used high-throughput methods are yeast two-hybrid screening and affinity purification coupled to mass spectrometry. This system...

the relative levels of the various proteins present in a cell, and two-hybrid screening, which allows the systematic exploration of protein–protein interactions...

Synthetic genetic array Virtual high throughput screening Yeast two-hybrid screening Zhao, Yicheng; Heumueller, Thomas; Zhang, Jiyun; Luo, Junsheng; Kasian...

The GAL4/UAS system is also utilized in Two-Hybrid Screening, a method of identifying interactions between two proteins or a protein with DNA. Gal4 expression...

yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain is sold commercially by Clontech and is used as a partner...

thought to interact with the protein encoded by LSMEM1 based on two-hybrid screening. Anton blood group antigen GRCh38: Ensembl release 89: ENSG00000172005...

primary site for iron absorption is the duodenum, where dietary iron exists in two forms: heme iron, sourced from animal products, and non-heme iron, found...

gene – locus – trait – allele – polymorphism – homozygote – heterozygote – hybrid – hybridization – dihybrid cross – Punnett square – inbreeding genotype–phenotype...

interest to other researchers. Data produced by microarray experiments, two-hybrid screening, and other high-throughput biological experiments is voluminous and...

Dysbindin was discovered by the research group of Derek Blake via yeast two-hybrid screening for binding partners of α-dystrobrevin. In addition, dysbindin is...

edges. PINs can be discovered with a variety of methods including; Two-hybrid Screening, in vitro: co-immunoprecipitation, blue native gel electrophoresis...

knockdown of a single gene on the expression of multiple genes. A yeast two-hybrid screening (Y2H) tests a "bait" protein against many potential interacting proteins...

yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain has been sold commercially by Clontech since at least...

large scale is in two-hybrid screening, which aims to identify proteins that natively interact with one another in vivo.The yeast two-hybrid (Y2H) system,...

huntingtin interacting protein 1 (HIP1), these were typically found using two-hybrid screening and confirmed using immunoprecipitation. Huntingtin has also been...

D (November 2000). "Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1...

blot Southern blot Restriction enzyme Polymerase chain reaction Two-hybrid screening in vivo in vitro in silico Field techniques Belt transect mark and...

Fields 1976 – biologist and HHMI investigator known for pioneering two-hybrid screening for discovering protein–protein interactions Walter W. Granger (honorary...

and cause cell-mediated, virus-induced apoptosis. Using a yeast two-hybrid screening system of ORF3 against the porcine cDNA library indicated that the...

p11 (annexin II light chain). It has been shown using the yeast-two hybrid screening method that p11 binds to a 28-amino-acid fragment at the N terminus...

DE (March 2006). "Identification of novel ARF binding proteins by two-hybrid screening". Cell Cycle. 5 (6): 641–6. doi:10.4161/cc.5.6.2560. PMID 16582619...

(November 2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs"....

contains one protein domain of unknown function, DUF1693. Yeast two-hybrid screening has identified three proteins that physically interact with FAM46B...

Kaiser C, Klink M, Suckow J, et al. (February 2005). "Automated yeast two-hybrid screening for nuclear receptor-interacting proteins". Molecular & Cellular...

(Nov 2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs"....

"Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening". Biochemistry. 39 (12): 3185–3191. doi:10.1021/bi992664v. PMID 10727209...

it has a function in DNA damage repair. It was discovered through two-hybrid screening during a search for binding partners of PARP9 (formerly BAL), a gene...

O (2003). "Protein–Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a Functionally Classified Library Composed of Long cDNAs"....

proteins interacting with transcription factor Sp1 identified in a two-hybrid screening". Molecular and Cellular Biochemistry. 210 (1–2): 131–142. doi:10...