Aminoallyl nucleotide is a nucleotide with a modified base containing an allylamine. They are used in post-labeling of nucleic acids by fluorescence detection...
13 KB (1,413 words) - 15:01, 8 January 2024
used as fluorescent probes, either directly or indirectly, such as aminoallyl nucleotide, which are used to label cRNA or cDNA in microarrays. Several groups...
15 KB (1,434 words) - 17:28, 1 July 2024
the coupling stage occurs before hybridization, using aminoallyl uridine triphosphate (aminoallyl-UTP, or aaUTP) and NHS amino-reactive dyes (such as cyanine...
54 KB (5,427 words) - 14:19, 26 April 2024
with a reactive fluorophore (indirect labelling): Amine reactive: aminoallyl nucleotides contain a primary amine group on a linker that reacts with the amino-reactive...
49 KB (5,301 words) - 01:33, 26 June 2024
nucleic acids lack, nucleotides have to be modified with aminoallyl groups. This is done through incorporating aminoallyl-modified nucleotides during synthesis...
17 KB (1,827 words) - 07:44, 7 June 2024
packed into a short string of DNA, as short as 15-20 nucleotides long for the mouse brain or 5 nucleotides for targeted cancer gene panels. In FISSEQ, a series...
6 KB (734 words) - 20:10, 1 November 2023