BufferGel is the brand name of a spermicide and microbicide gel which is being tested for its potential development into a preventive medicine to stop...
3 KB (278 words) - 10:27, 14 August 2023
A gel is a semi-solid that can have properties ranging from soft and weak to hard and tough. Gels are defined as a substantially dilute cross-linked system...
37 KB (4,587 words) - 10:46, 29 October 2024
electrophoretic buffers by substituting TBE and TAE buffers for a more efficient and inexpensive conductive media in gel systems. TAE (Tris-acetate-EDTA) buffer is...
4 KB (540 words) - 18:20, 28 October 2023
passing a current through a gel causes heating, gels may melt during electrophoresis. Electrophoresis is performed in buffer solutions to reduce pH changes...
44 KB (5,065 words) - 05:47, 30 October 2024
extracted from the gel with relative ease. Most agarose gels used are between 0.7–2% dissolved in a suitable electrophoresis buffer. Agarose gel is a three-dimensional...
34 KB (4,138 words) - 15:43, 31 October 2024
disposable diaphragm is made of dipped polyurethane, pre-filled with BufferGel (BufferGel is currently in clinical trials as a spermicide and microbicide)...
42 KB (4,520 words) - 17:04, 7 October 2024
(or DISC) buffer system that significantly enhances the sharpness of the bands within the gel. During electrophoresis in a discontinuous gel system, an...
20 KB (2,351 words) - 06:33, 18 April 2024
biochemistry and molecular biology, saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for...
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SB buffer is a buffer solution used in agarose and polyacrylamide gel electrophoresis for the separation of nucleic acids such as DNA and RNA. "SB" is...
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Microbicides for sexually transmitted infections (redirect from Viva Gel)
inactivate) pathogenic microorganisms. One such agent is BufferGel, a spermicidal and microbicidal gel formulated to maintain the natural protective acidity...
32 KB (3,411 words) - 07:29, 27 December 2023
ethanol, surfactants etc. Buffers can be added to control the pH of aqueous or hydroalcoholic based gels. Examples of buffers include phosphate and citrate...
29 KB (2,760 words) - 14:48, 18 July 2024
Silica gel is an amorphous and porous form of silicon dioxide (silica), consisting of an irregular tridimensional framework of alternating silicon and...
22 KB (2,365 words) - 13:56, 18 October 2024
another one suitable for a downstream application (buffer exchange). These methods are based on gel filtration chromatography, also called molecular sieve...
8 KB (1,032 words) - 23:20, 21 June 2024
SDS-PAGE (redirect from SDS-polyacrylamide gel electrophoresis)
pre-cast gels) usually use the buffer substance Bis-tris methane with a pH value between 6.4 and 7.2 both in the stacking gel and in the separating gel. These...
42 KB (5,319 words) - 04:03, 22 June 2024
the gel during the electrophoretic run. The gels typically consist of acrylamide, bisacrylamide, the optional denaturant (SDS or urea), and a buffer with...
36 KB (4,717 words) - 11:43, 31 October 2024
and physical properties of ACES buffer. ACES had been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. ACES use in isoelectric...
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biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis...
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thus the gel temperature is much lower than with TBE/TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes...
3 KB (339 words) - 14:32, 22 November 2023
paper soaked in transfer buffer | gel | PVDF or nitrocellulose membrane | three sheets of filter paper soaked in transfer buffer | sponge. It is a necessity...
4 KB (395 words) - 00:59, 18 June 2024
lubricating gel. A "tight buffer" consists of a polymer coating in intimate contact with the primary coating applied to the fiber during manufacture. Buffer application...
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migration of nucleic acids: the dimension of the gel pores, the voltage used, the ionic strength of the buffer, and the concentration intercalating dye such...
24 KB (3,131 words) - 14:50, 31 October 2024
Size-exclusion chromatography (redirect from Gel filtration chromatography)
sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic...
31 KB (3,901 words) - 05:01, 30 October 2024
for one of these buffers, as the DNA will not migrate along the gel. Furthermore, using water instead of buffer will result in the gel melting. Charge/Voltage...
41 KB (4,361 words) - 06:13, 31 July 2024
fairly constant relative humidity, between 55 and 70%. Silica gel is widely used to buffer relative humidity inside museum display cases, packaged clothing...
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2-Hydroxy-3-morpholinopropanesulfonic acid (redirect from MOPSO (buffer))
Discontinuous gel electrophoresis on rehydratable polyacrylamide gels Buffered charcoal yeast extract agar Fixing cells in urine in a buffered alcohol Testing...
3 KB (189 words) - 21:27, 6 November 2023
Temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE) are forms of electrophoresis which use either a temperature...
11 KB (1,639 words) - 18:58, 4 February 2024
MOPS (category Buffer solutions)
is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above...
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top of the membrane and gel), to ensure good and even contact between gel and membrane. If transferring by suction, 20X SSC buffer is used to ensure a seal...
17 KB (2,309 words) - 13:53, 2 October 2024
from the gel most protocols require a destaining of the proteins before proceeding. The destaining solution for CBB contains usually the buffer salt ammonium...
20 KB (2,749 words) - 02:26, 19 February 2024
Western blot (section Gel electrophoresis)
sample and the nature of the gel. By far the most common type of gel electrophoresis employs polyacrylamide gels and buffers loaded with sodium dodecyl...
66 KB (8,092 words) - 04:53, 30 October 2024