Chimeric nucleases are an example of engineered proteins which must comprise a DNA-binding domain to give sequence specificity and a nuclease domain for...

frequency. Chimeric nuclease Genome editing Gene targeting Zinc finger protein Zinc finger chimera Protein engineering Zinc finger nuclease treatment of...

current nuclease-based gene editing platforms but its use was limited by low efficiencies of editing. Genome editing with engineered nucleases, i.e. all...

the first to demonstrate precise gene editing in human cells using chimeric nucleases. He graduated magna cum laude from Harvard University and completed...

the first to demonstrate precise gene editing in human cells using chimeric nucleases. In October 2005, Baltimore resigned the office of the president of...

contrast, the in vivo sample is likely to have host antibodies, proteases, nucleases, nucleic acids and viruses. In both cases, other secretions by the hybridomas...

Cas9 contains two nuclease domains: an McrA-like HNH nuclease domain and a RuvC-like nuclease domain. These HNH and RuvC-like nuclease domains are responsible...

needed] Transcription activator-like effector nucleases (TALENs) also contain a DNA binding domain and a nuclease that can cleave DNA. The DNA binding region...

finger nucleases. Since 2009 more accurate and easier systems to implement have been developed. Transcription activator-like effector nucleases (TALENs)...

than other cellular adoptive immunotherapies such as CAR T cell therapy. Chimeric antigen receptor T-cells (CAR-T) are predominantly used in cancer immunotherapy...

"Stimulation of homologous recombination through targeted cleavage by chimeric nucleases". Mol Cell Biol. 21 (1): 289–297. doi:10.1128/MCB.21.1.289-297.2001...

biotechnology company Tiziana Life Sciences to explore using foralumab to aid chimeric antigen receptor (CAR) T cell therapy, and with Philadelphia-based iECURE...

interacting loci are re-ligated together to create a genomic library of chimeric DNA molecules. The relative abundance of these chimeras, or ligation products...

company, has developed a fully rational design process called Directed Nuclease Editor (DNE) which is capable of creating engineered meganucleases that...

the Department of Genetics. This work represented the first use of chimeric nucleases to edit genomes by homologous recombination. Later, while working...

collaborating groups of Emmanuelle Charpentier and Jennifer Doudna described a chimeric RNA design which is capable of facilitating cleavage of DNA using purified...

viral replication and protecting the 3'UTR from 5' endonuclease digestion. Nuclease resistance protects the downstream 3' UTR RNA fragment from degradation...

different parental genes priming for each other, resulting in chimeric DNA. The chimeric DNA of parental size is then amplified using end terminal primers...

increased understanding of nuclease function has led to more direct DNA editing, using techniques such as zinc finger nucleases and CRISPR. The vector incorporates...

the targeted DNA region. Hence the genes encoding for the site-specific-nuclease of interest may also be transformed along with the repair template. These...

Zinc finger protein chimera are chimeric proteins composed of a DNA-binding zinc finger protein domain and another domain through which the protein exerts...

combine its CRISPR-Cas9 technology with Juno's experience in creating chimeric antigen receptor and high-affinity T cell receptor therapeutics to the...

Sgs1 helicase and the Exo1 and Dna2 nucleases. As a helicase, Sgs1 "unzips" the double-strand DNA, while the nuclease activity of Exo1 and Dna2 allows them...

base pairing occurs, other proteins direct the mRNA to be destroyed by nucleases. Next to be linked to regulation were Xist and other long noncoding RNAs...

fashion with the target DNA. Information retrieved from experiments with chimeric integrases show that recognition of the target site is controlled by the...

(the nuclease Cas9) and (b) the DNA sequences of previously encountered viral invaders (CRISPR). These viral DNA sequences enable the nuclease to target...

base-pairing of a single guide RNA (sgRNA) to the genomic locus. sgRNA is a chimeric noncoding RNA that can be subdivided into three regions: a 20 nt base-pairing...

offer an editorial comment in the journal Gene: The work on restriction nucleases not only permits us easily to construct recombinant DNA molecules and...

oncogenic chimeric proteins, the fusion product lacks functional domain from respective genes, indicative of an amorphic rearrangement. This chimeric LACTB2-NCOA2...

position corresponded to 30% of the total DNA sequence of the yeast HO nuclease. Many genes have unrelated intein-coding segments inserted at different...