The isoelectric point (pI, pH(I), IEP), is the pH at which a molecule carries no net electrical charge or is electrically neutral in the statistical mean...
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Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point...
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salt effect (pzse), zero point of titration (zpt) of colloidal dispersion, and isoelectric point of the solid (ieps) and point of zero surface tension...
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Isoelectric may refer to: Isoelectric point, the pH at which a particular molecule carries no net electrical charge Isoelectric focusing, a technique...
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Amino acid (section Isoelectric point)
net charge of all forms present is zero. This pH is known as the isoelectric point pI, so pI = 1/2(pKa1 + pKa2). For amino acids with charged side...
98 KB (10,151 words) - 03:53, 21 October 2024
Acid dissociation constant (section Isoelectric point)
disorder. The isoelectric point of a given molecule is a function of its pK values, so different molecules have different isoelectric points. This permits...
103 KB (11,513 words) - 22:40, 15 October 2024
Protein precipitation (redirect from Isoelectric precipitation)
disadvantage to isoelectric point precipitation is the irreversible denaturation caused by the mineral acids. For this reason isoelectric point precipitation...
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maintains its activity in a large range of pH (6–9). Its isoelectric point is 11.35. The isoelectric point of human milk lysozyme is 10.5–11. The enzyme functions...
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whey protein is covering the casein micelle, isoelectric point of the micelle elevated to the isoelectric point of β lactoglobulin (approximately pH 5.3)...
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according to isoelectric point, while in the second dimension, polypeptides are separated according to their molecular weight. The isoelectric point of a given...
66 KB (8,092 words) - 16:32, 2 November 2024
is known as the molecule's isoelectric point. Ampholytes are used to establish a stable pH gradient for use in isoelectric focusing. Metal oxides which...
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EVA1C (section Isoelectric point and molecular weight)
region of the midbrain in the house mouse (Mus musculus) brain. The isoelectric point of the EVA1C protein in humans (Homo sapiens) is 6.5 pl and the molecular...
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chromatography must be done in conditions that are one pH unit away from the isoelectric point of a protein. The two types of ion chromatography are anion-exchange...
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technique can be isoelectric point, protein complex mass in the native state, or protein mass.[citation needed] The separation by isoelectric point is called...
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help bring the proteins near their isoelectric point to allow them to be denatured easier. The isoelectric point is a specific pH where a molecule, in...
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separated along with the anions based on their isoelectric point (pI). In proteins, the isoelectric point (pI) is defined as the pH at which a protein has...
48 KB (4,964 words) - 04:26, 20 October 2024
(126–145 °F)[citation needed] at the moment the cheese is at the isoelectric point, meaning the state at which half the ionizable surface amino acids...
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Its main function is to regulate the oncotic pressure of blood. The isoelectric point of albumin is 4.7. Alpha-fetoprotein is a fetal plasma protein that...
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S2CID 9269742. Kozlowski, LP (26 October 2016). "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research. 45 (D1): D1112–D1116. doi:10.1093/nar/gkw978...
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of 6.7; however, the pH of the cream must drop to below 4.6, the isoelectric point of milk, for the casein micelles to fully precipitate out of solution...
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gel electrophoresis if the desired protein's molecular weight and isoelectric point are known, by spectroscopy if the protein has distinguishable spectroscopic...
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isoelectric point (pI) in that pI is the pH value at which the net charge of the molecule, including bound ions is zero. Whereas the isoionic point is...
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Archived from the original on 15 June 2012. Retrieved 17 May 2012. For isoelectric point: Gayer, K. H.; Thompson, L. C.; Zajicek, O. T. (September 1958). "The...
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might change the size or isoelectric point of proteins. The binary shifts might be left to right (change in isoelectric point), vertical (change in size)...
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the egg white proteins are near their isoelectric points. Protein adsorption is most rapid at the isoelectric point because electrostatic repulsion is reduced...
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mammalian species and is a homogeneous 50 kDa glycoprotein with an isoelectric point of 6.5. The protein is prepared in a lyophilized 1 microgram dose...
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combinatorics Integrated electric propulsion, in marine propulsion Isoelectric point, the pH where a molecule is electrically neutral Individualized Education...
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proteins, H. salinarum encodes mainly acidic proteins. The average isoelectric point of H. salinarum proteins is 5.03. These highly acidic proteins are...
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further analyzed by isoelectric focusing (IEF) in the pH range 4.5-5.5, where the protein migrates in a gel according to its isoelectric point or charge in a...
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mg/mL at 20°C log P -1.76 Acidity (pKa) 12.309 Basicity (pKb) 1.688 Isoelectric point 11.19 Thermochemistry Heat capacity (C) 138.1 J K−1 mol−1 (at 23.4 °C)...
22 KB (2,387 words) - 03:45, 27 October 2024