• Thumbnail for Real-time polymerase chain reaction
    (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors...
    43 KB (5,338 words) - 11:09, 12 July 2024
  • Thumbnail for Reverse transcription polymerase chain reaction
    real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article, RT-PCR will...
    43 KB (5,536 words) - 07:35, 12 July 2024
  • Thumbnail for Polymerase chain reaction
    the body of English king Richard III. Quantitative PCR or Real Time PCR (qPCR, not to be confused with RT-PCR) methods allow the estimation of the amount...
    89 KB (11,117 words) - 12:33, 8 July 2024
  • A quantitative PCR instrument is a machine that amplifies and detects DNA. It combines the functions of a thermal cycler and a fluorimeter, enabling the...
    10 KB (1,008 words) - 19:36, 1 May 2024
  • advantage of quantitative PCR is that analysis of resulting products using gel electrophoresis is not required. This means that quantitative PCR can be implemented...
    38 KB (5,658 words) - 00:49, 1 July 2024
  • Thumbnail for Bisulfite sequencing
    high-resolution melting analysis (HRM), a quantitative PCR-based technique initially designed to distinguish SNPs. The PCR amplicons are analyzed directly by...
    34 KB (4,084 words) - 21:59, 8 January 2024
  • hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher Kary Mullis at...
    6 KB (684 words) - 02:15, 23 December 2023
  • Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments...
    20 KB (2,673 words) - 11:49, 9 July 2024
  • Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction...
    68 KB (7,294 words) - 14:12, 27 June 2024
  • 2003). "Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis". Proceedings of the...
    3 KB (334 words) - 20:53, 13 September 2023
  • PMC 2642996. PMID 19240792. Abbs, S; Bobrow, M (1992). "Analysis of quantitative PCR for the diagnosis of deletion and duplication carriers in the dystrophin...
    7 KB (763 words) - 05:22, 9 June 2024
  • Thumbnail for Thermal cycler
    Thermal cycler (redirect from PCR machine)
    and cold zones on a microfluidic chip. Thermal cyclers designed for quantitative PCR have optical systems which enable fluorescence to be monitored during...
    4 KB (493 words) - 09:01, 21 May 2024
  • product during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods...
    32 KB (4,102 words) - 05:25, 7 February 2024
  • Thumbnail for Aneuploidy
    hybridization (FISH), quantitative PCR of short tandem repeats, quantitative fluorescence PCR (QF-PCR), quantitative PCR dosage analysis, Quantitative Mass Spectrometry...
    36 KB (3,424 words) - 07:27, 4 June 2024
  • competition for PCR reagents, thus potentially inhibiting amplification of the DNA sequence targeted for PCR amplification. In quantitative PCR, PDs may interfere...
    14 KB (1,573 words) - 10:45, 9 July 2024
  • Viability PCR, also named v-PCR or vPCR, is an evolution of PCR. Through the use of a simple pre-treatment of the sample by the means of specific intercalating...
    6 KB (742 words) - 13:01, 8 December 2023
  • Thumbnail for RNase H-dependent PCR
    PCR reaction. These primers can be used in both 5’ nuclease Taqman and SYBR Green types of quantitative PCR. rhPCR can be used for quantitative PCR and...
    5 KB (596 words) - 01:29, 3 December 2023
  • Thumbnail for COVID-19 testing
    as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used. The Minimum...
    181 KB (21,044 words) - 08:55, 1 July 2024
  • Thumbnail for Human herpesvirus 6
    viral DNA in blood, other body fluids, and organs by means of real-time PCR. The classical presentation of primary HHV-6b infection is as exanthema subitum...
    78 KB (8,112 words) - 16:36, 5 July 2024
  • down a station Quantitative PCR or qrt-PCR, a laboratory DNA detection technique Qube Research & Technologies, London-based quantitative investment management...
    581 bytes (121 words) - 04:48, 12 February 2024
  • Thumbnail for Amplified fragment length polymorphism
    Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic...
    7 KB (708 words) - 07:57, 22 February 2024
  • Thumbnail for Kary Mullis
    reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry with Michael Smith and was awarded the Japan Prize in the same year. PCR became a...
    47 KB (4,851 words) - 21:22, 8 May 2024
  • Thumbnail for Psittacine beak and feather disease
    presence of BFDV are available: standard polymerase chain reaction (PCR), quantitative PCR (qPCR) which can detect the virus in extremely small quantities, whole-genome...
    40 KB (4,430 words) - 01:52, 20 May 2024
  • Thumbnail for In silico PCR
    multiplex PCR, allowing for an estimate of quantitative competition effects between multiple amplicons in one reaction. The UCSC Genome Browser offers isPCR, which...
    8 KB (867 words) - 17:06, 11 June 2024
  • Thumbnail for Combined bisulfite restriction analysis
    restriction enzyme digestion followed by PCR. Quantitative: This is in contrast to methylation-specific PCR, which is qualitative. With COBRA, DNA methylation...
    12 KB (1,300 words) - 12:11, 5 June 2023
  • DNA molecule. These tags are added before PCR amplification, and can be used to reduce errors and quantitative bias introduced by the amplification. Applications...
    2 KB (230 words) - 12:51, 27 April 2024
  • of interest to a broad readership. The journal was established in 1991 as PCR Methods and Applications and obtained its current title in 1995. According...
    3 KB (190 words) - 10:49, 24 June 2024
  • Resolution Melting (dHRM) is also used in conjunction with digital PCR (dPCR) to improve quantitative power by providing additional information on the melting behavior...
    12 KB (1,448 words) - 07:05, 11 March 2024
  • quantitative real time PCR.[citation needed] cffDNA may be detected by finding paternally inherited DNA sequences via polymerase chain reaction (PCR)...
    51 KB (5,952 words) - 19:05, 6 May 2024
  • Thumbnail for Kompetitive allele specific PCR
    Kompetitive allele specific PCR (KASP) is a homogenous, fluorescence-based genotyping variant of polymerase chain reaction. It is based on allele-specific...
    7 KB (988 words) - 10:29, 10 January 2024