Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a...
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Race (section Rapid movement)
Emergency Service, in the US, established in 1952 for wartime use Rapid amplification of cDNA ends, a technique in molecular biology RACE (Remote Applications...
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Polymerase chain reaction (redirect from PCR amplification)
millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently...
89 KB (11,135 words) - 11:52, 26 August 2024
Tcr-seq (category DNA sequencing)
PMID 34282327. Yeku, Oladapo; Frohman, Michael A. (2011). "Rapid Amplification of cDNA Ends (RACE)". RNA. Methods in Molecular Biology. Vol. 703. pp. 107–122...
22 KB (2,554 words) - 14:34, 22 July 2024
also referred to as parallel analysis of RNA ends (PARE), is a modified version of 5'-Rapid Amplification of cDNA Ends (RACE) using high-throughput, deep...
22 KB (2,432 words) - 12:26, 22 February 2024
Random amplified polymorphic DNA (RAPD), pronounced "rapid", is a type of polymerase chain reaction (PCR), but the segments of DNA that are amplified are...
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Loop-mediated isothermal amplification (LAMP) is a single-tube technique for the amplification of DNA for diagnostic purposes and a low-cost alternative...
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complement each (template) strand. DNA replication occurs during the S-stage of interphase. DNA replication (DNA amplification) can also be performed in vitro...
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genome and the location of these non-coding RNAs within the bacterial genome was confirmed by RACE (rapid amplification of cDNA ends) analysis. These studies...
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further amplification via PCR. After adaptor ligation, cDNA are cleaved using TE to remove them from the beads, leaving only a short "tag" of about 11...
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HERC2 (section DNA repair pathways)
Au-Young J, Stuve LL (July 2004). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics. 84 (1): 205–10...
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5'-end, for instance in protocols for RACE (Rapid Amplification of cDNA Ends). Commercial production of TAP was discontinued in 2015, however Cap-Clip acid...
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MicroRNA sequencing (category DNA sequencing)
Validation of target cleavage in specific mRNAs is typically performed using a modified version of 5' Rapid Amplification of cDNA Ends with a gene-specific...
49 KB (5,200 words) - 07:39, 12 December 2023
loop-mediated isothermal amplification (RT-LAMP) is a one step nucleic acid amplification method to multiply specific sequences of RNA. It is used to diagnose...
15 KB (1,765 words) - 01:04, 18 June 2024
JT, Yang J, et al. (2005). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics. 84 (1): 205–10...
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RuBisCO (section C-C bond cleavage)
"Determination of the relative expression levels of rubisco small subunit genes in Arabidopsis by rapid amplification of cDNA ends". Analytical Biochemistry...
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complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both...
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Rolling circle replication (redirect from Rolling circle amplification)
simplified version of natural rolling circle replication, an isothermal DNA amplification technique, rolling circle amplification was developed. The RCA...
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(August 1993). "Cloning of the 5' mRNA for the 230-kD bullous pemphigoid antigen by rapid amplification of cDNA ends". The Journal of Investigative Dermatology...
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Au-Young J, Stuve LL (July 2004). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics. 84 (1): 205–10...
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combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase...
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KCNK2 (category Wikipedia articles incorporating text from the United States National Library of Medicine)
JT, Yang J, et al. (2005). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics. 84 (1): 205–10...
12 KB (1,429 words) - 17:48, 27 December 2023
Real-time polymerase chain reaction (section Non-specific detection: real-time PCR with double-stranded DNA-binding dyes as reporters)
laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the...
43 KB (5,338 words) - 11:09, 12 July 2024
JT, Yang J, et al. (2005). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics. 84 (1): 205–10...
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Removal of these inhibitors is crucial to ensure that high quality DNA is available for subsequent analyzing. Amplification of the extracted DNA is a required...
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that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated...
126 KB (14,372 words) - 20:35, 28 August 2024
Au-Young J, Stuve LL (July 2004). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics. 84 (1): 205–10...
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polymerase chain reaction and related methods for the amplification and modification of DNA. Several DNA polymerases have been described with distinct properties...
41 KB (5,164 words) - 23:57, 28 July 2024
Au-Young J, Stuve LL (2005). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics. 84 (1): 205–10...
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early in adipocyte differentiation. The full length cDNA was cloned by rapid amplification of cDNA ends method and sequence analysis results in a protein...
18 KB (2,258 words) - 22:26, 27 March 2024