• In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events. It can be formed artificially...
    13 KB (1,398 words) - 10:21, 9 July 2024
  • Thumbnail for Amplicon sequence variant
    An amplicon sequence variant (ASV) is any one of the inferred single DNA sequences recovered from a high-throughput analysis of marker genes. Because these...
    8 KB (767 words) - 07:34, 22 February 2024
  • sequencing (sometimes called target amplicon sequencing) (TAS) is a next-generation DNA sequencing technique focusing on amplicons and specific genes. It is useful...
    2 KB (202 words) - 20:53, 30 March 2021
  • Thumbnail for Runhild Gammelsæter
    portraits by Runhild herself. On June 28, 2008, her debut solo album, Amplicon, was released through Utech Records. On December 13, 2014, she released...
    5 KB (347 words) - 13:30, 3 July 2024
  • Thumbnail for Y chromosome
    Found in the "yellow" amplicon. HSFY1, HSFY2 HSFX1, HSFX2 Found in the "blue" amplicon. PRY, PRY2 Found in the "blue" amplicon. Identified by similarity...
    81 KB (8,127 words) - 11:18, 23 June 2024
  • denaturation of the entire dsDNA amplicon. You make primers specific to the site you want to amplify. You "paint" the amplicon with a double-strand specific...
    37 KB (5,002 words) - 04:04, 22 June 2024
  • Thumbnail for Circulating tumor DNA
    sensitivity of CAPP-Seq is reported to be 2.5 molecules in 1,000,000. Tagged amplicon deep sequencing (TAM-Seq) allows targeted sequencing of entire genes to...
    49 KB (5,856 words) - 05:57, 9 April 2024
  • Thumbnail for Bisulfite sequencing
    quantitative PCR-based technique initially designed to distinguish SNPs. The PCR amplicons are analyzed directly by temperature ramping and resulting liberation...
    34 KB (4,084 words) - 21:59, 8 January 2024
  • Thumbnail for Transcription-mediated amplification
    polymerase) and DNA synthesis (via reverse transcriptase) to produce an RNA amplicon (the source or product of amplification) from a target nucleic acid. This...
    2 KB (282 words) - 22:31, 30 January 2023
  • Thumbnail for Microbiota
    Targeted amplicon sequencing relies on having some expectations about the composition of the community that is being studied. In target amplicon sequencing...
    68 KB (7,825 words) - 20:49, 15 June 2024
  • consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting...
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  • Thumbnail for Haplogroup R2
    Common Name Marker M479 YCC Haplogroup R-M479 Nucleotide change C to T Amplicon size (bp) reference sequence 323 Polymorphism position from 5' end 107...
    6 KB (470 words) - 10:27, 11 July 2024
  • Thumbnail for Varicella zoster virus
    two minor genotypes of varicella-zoster virus strains: a practical two-amplicon approach used to genotype clinical isolates in Australia and New Zealand"...
    26 KB (3,079 words) - 11:21, 7 May 2024
  • Thumbnail for Polymerase chain reaction
    anticipated DNA target region (also sometimes referred to as the amplimer or amplicon), agarose gel electrophoresis may be employed for size separation of the...
    89 KB (11,117 words) - 12:33, 8 July 2024
  • template for subsequent cycles), MALBAC utilizes special primers that allow amplicons to have complementary ends and therefore to loop, preventing DNA from...
    14 KB (1,955 words) - 00:17, 24 November 2022
  • Thumbnail for 16S ribosomal RNA
    referred to as 27F and 1492R; however, for some applications shorter amplicons may be necessary, for example for 454 sequencing with titanium chemistry...
    34 KB (3,796 words) - 19:13, 3 July 2024
  • Thumbnail for Viral metagenomics
    untargeted metagenomics and metatranscriptomics does not need a genetic marker, amplicon sequencing does. It uses a gene that is highly conserved as a genetic marker...
    26 KB (2,872 words) - 13:45, 2 March 2024
  • amplified is known as the amplicon. After the PCR process the HRM analysis begins. The process is simply a precise warming of the amplicon DNA from around 50...
    18 KB (2,573 words) - 07:24, 3 December 2023
  • ESV, a brand of Cadillac Escalade Exact sequence variant, also called an amplicon sequence variant Experimental safety vehicle M1132 Engineer Squad Vehicle...
    569 bytes (89 words) - 04:10, 28 January 2024
  • Thumbnail for DNA sequencing
    "Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae...
    129 KB (14,413 words) - 10:39, 30 May 2024
  • Thumbnail for Mangrove
    differentiation of root-associated microbial communities, Nevertheless, amplicon-based community profiling may not provide the functional characteristics...
    113 KB (10,782 words) - 05:28, 10 July 2024
  • provide very specific melting curves from the single-stranded (ss) probe-to-amplicon hybrid. Idaho Technology and Roche have done much to popularize this use...
    12 KB (1,448 words) - 07:05, 11 March 2024
  • Microsatellites can be analysed and verified by established PCR amplification and amplicon size determination, sometimes followed by Sanger DNA sequencing. In forensics...
    67 KB (7,393 words) - 17:16, 28 June 2024
  • Thumbnail for Fluorescent in situ sequencing
    acid sequencing library construction method that stably cross-links cDNA amplicons within biological samples. Sequencing data is then generated through an...
    6 KB (734 words) - 20:10, 1 November 2023
  • Thumbnail for DNA sequencer
    and complex genomes of any size. Finally, the GS Amplicon Variant Analyzer aligns reads from amplicon samples against a reference, identifying variants...
    37 KB (3,850 words) - 23:38, 23 March 2024
  • the polymerase chain reaction (PCR). It is primarily used to generate amplicon libraries for DNA sequencing by next generation sequencing (NGS). The technique...
    13 KB (1,366 words) - 11:32, 9 July 2024
  • method combined with amplicon detection by AuNP can have advantages over other methods in terms of reduced assay time, amplicon confirmation by hybridization...
    26 KB (3,025 words) - 15:17, 1 July 2024
  • Thumbnail for Genetics
    Li B, Feng Q, Shen Z, Lee CH, et al. (8 June 2017). "Detection of DNA Amplicons of Polymerase Chain Reaction Using Litmus Test". Scientific Reports. 7...
    98 KB (10,398 words) - 19:27, 28 June 2024
  • Thumbnail for DNA methylation
    Pyrosequencing of bisulfite treated DNA. This is the sequencing of an amplicon made by a normal forward primer but a biotinylated reverse primer to PCR...
    114 KB (13,198 words) - 18:21, 21 June 2024
  • Thumbnail for DNA barcoding
    sources. However, some studies argue that there is no relationship between amplicon size and detection rate of eDNA. When the DNA barcode marker region has...
    99 KB (11,142 words) - 01:40, 29 June 2024