• Thumbnail for Dot blot
    A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with...
    3 KB (439 words) - 03:43, 23 March 2024
  • Thumbnail for Western blot
    specific target protein. Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry and immunocytochemistry, where antibodies...
    66 KB (8,077 words) - 10:16, 9 July 2024
  • Thumbnail for Blot (biology)
    probes. A dot blot is a special case of any of the above blots where the analyte is added directly to the blotting matrix (and appears as a "dot") as opposed...
    6 KB (694 words) - 21:55, 28 March 2024
  • probe for the presence of the target in a Southern blot assay or, more commonly, in the simpler dot blot assay. It is a common tool used in genetic testing...
    10 KB (1,292 words) - 20:49, 18 September 2023
  • Thumbnail for Monoclonal antibody
    antigen (with a test such as ELISA or antigen microarray assay) or immuno-dot blot. The most productive and stable clone is then selected for future use....
    47 KB (4,802 words) - 02:10, 28 June 2024
  • oligonucleotide (ASO) probes, a process that can often be done by a simple dot blot. Amplified fragment length polymorphism (AFLP) RAPD STR analysis Saiki...
    9 KB (1,159 words) - 04:36, 23 May 2023
  • Thumbnail for Angiostrongylus cantonensis
    antigen-antibody reactions are being explored, such as immuno-PCR. A rapid dot-blot ELISA test is also available for quick, effective, and economical on-site...
    44 KB (4,577 words) - 15:56, 22 April 2024
  • chemically modified paper filters or microporous membrane filters. In a dot blot, macromolecules are applied directly to the matrix. Macromolecules can...
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  • functionally mapping epitopes often use binding assays such as western blot, dot blot, and/or ELISA to determine antibody binding. Competition methods look...
    21 KB (2,397 words) - 06:13, 26 February 2024
  • affinity purification Protein:protein interaction studies Colony blot, dot blot, Western blot and ELISA Screening for positive expression clones Immunocytochemistry...
    8 KB (1,027 words) - 04:19, 22 June 2024
  • oligonucleotide (ASO) probes, a process that can often be done by a simple dot blot. RFLP https://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechCAPS.shtml...
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  • Thumbnail for Allium
    Allium L. subgenus Rhizirideum (G. Don ex Koch) Wendelbo according to dot blot hybridization with randomly amplified DNA probes". Theoretical and Applied...
    49 KB (4,743 words) - 03:39, 30 June 2024
  • Thumbnail for E. L. Moore
    Railroader. On a calendar he would mark a dot for each hour worked, half a dot for a half hour, and a blot for anything thing that seemed like 3 hours...
    36 KB (2,833 words) - 21:42, 21 May 2022
  • Thumbnail for Proteomics
    analyzed, either a very specific antibody needs to be used in quantitative dot blot analysis (QDB), or biochemical separation then needs to be used before...
    76 KB (8,908 words) - 20:18, 5 June 2024
  • rabbit antisera against representative strains with diverse origins and by Dot blot assays. These results endorsed the establishment of two different groups...
    15 KB (1,889 words) - 18:08, 19 December 2023
  • nylon membranes, which can be accomplished by use of dot blots or bidirectional agarose gel blotting and UV fixation of the DNA to the membranes. In many...
    10 KB (1,189 words) - 21:45, 10 November 2022
  • Thumbnail for Quantitative proteomics
    spectrometry (MS). However, a recent developed method of quantitative dot blot (QDB) analysis is able to measure both the absolute and relative quantity...
    28 KB (3,409 words) - 01:36, 19 May 2024
  • Thumbnail for History of polymerase chain reaction
    Enzymology vol. 155(F) pp. 335–50 (1987). Verlaan-de Vries M et al. "A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides...
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  • molecular diagnosis based in test samples. Some of these methods (like Dot-blot hybridisation) allow the scientists to detect the viroid even six months...
    23 KB (3,022 words) - 20:51, 30 June 2024
  • protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression levels in a large...
    23 KB (2,925 words) - 03:50, 28 January 2024
  • Thumbnail for Aldolase B
    (1999). "Screening for hereditary fructose intolerance mutations by reverse dot-blot". Mol. Cell. Probes. 13 (1): 35–40. doi:10.1006/mcpr.1998.0208. PMID 10024431...
    17 KB (2,239 words) - 03:19, 8 March 2024
  • Thumbnail for Thymidine kinase
    health screening study on 35,365 people, using a sensitive chemiluminescent dot blot assay". Sensors. 11 (12): 11064–80. Bibcode:2011Senso..1111064C. doi:10...
    103 KB (11,261 words) - 22:07, 19 July 2024
  • circovirus by using polymerase chain reaction (PCR)-based methods and dot blot hybridisation (DBH) tests. Infection with DuCV appears to cause growth...
    6 KB (659 words) - 17:50, 19 February 2022
  • and plants by the UP-PCR (Universally Primed PCR) technique and cross dot blot hybridization of PCR products". European Journal of Protistology. 35 (3):...
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  • DNA polymerase - DNA replication - DNA sequencing - DNase - dominant - dot blot - double helix - downstream (DNA) - downstream (transduction) - drimenol...
    22 KB (1,928 words) - 20:22, 8 April 2024
  • sequence 194-225, a direct dot-blot assay with chemiluminescence end point and a microtiter sandwich ELISA. The dot-blot assay is a nitrocellulose membrane...
    83 KB (9,990 words) - 04:54, 15 July 2024
  • measured by the cccDNA levels via Southern blot kinetics of healthy vs infected cells and quantified by dot blot. In these infected cells, there is a strong...
    21 KB (2,510 words) - 08:10, 11 January 2024
  • identify avian reoviruses molecularly by observing infected tissues with dot-blot hybridization, PCR, and a combination of PCR and RFLP. This combination...
    22 KB (2,924 words) - 09:30, 18 August 2023
  • increased sensitivity compared to methods such as RNase protection assays and dot blot hybridization. The disadvantage to RT-PCR is the requirement of costly...
    27 KB (2,898 words) - 05:34, 10 June 2024
  • stationary phase Reverse phase protein lysate microarray, a micro-cell lysate dot-blot that allows measurement of protein expression levels This disambiguation...
    287 bytes (68 words) - 18:42, 30 November 2009