An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis, also referred as a gel shift assay, gel mobility shift assay, band shift...
9 KB (1,131 words) - 00:15, 15 October 2024
Affinity electrophoresis (category Protein–protein interaction assays)
electrophoresis. The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis...
19 KB (2,124 words) - 04:22, 8 April 2024
Mass spectrometry Isotopic labeling Coimmunoprecipitation Electrophoretic mobility shift assay Southwestern blotting Biochemistry at Wikipedia's sister...
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Smithies' method is an improvement. History of electrophoresis Electrophoretic mobility shift assay Gel extraction Isoelectric focusing Pulsed field gel electrophoresis...
44 KB (5,065 words) - 05:47, 30 October 2024
effector complex-target interactions. Where the traditional Electrophoretic Mobility Shift Assay (EMSA) method can be used, BLI can act as a suitable substitute...
14 KB (1,579 words) - 04:30, 18 June 2024
which associate with the promoter can be identified using an electrophoretic mobility shift assay (EMSA), and the effects of inclusion or exclusion of the...
8 KB (1,005 words) - 02:28, 11 November 2024
antibodies. The sample is detected on a western blot. By using electrophoretic mobility shift assay (EMSA), the activation profile of transcription factors can...
74 KB (8,192 words) - 09:41, 10 November 2024
electrophoresis, capillary electrophoresis, electroblotting, electrophoretic mobility shift assay, free-flow electrophoresis, isotachophoresis, preparative...
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Interactions. The following lists some methods currently in use: Electrophoretic mobility shift assay (EMSA) is a widespread qualitative technique to study protein–DNA...
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acrylamide gel, is isolated by autoradiography as per an Electrophoretic mobility shift assay (EMSA) protocol. Amplify the bound template by PCR. For positive...
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and shape information for the nucleic acid complexes. An electrophoretic mobility shift assay can assess whether a structure incorporates all desired strands...
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isolated from a human telomeric sequence. The study utilized an electrophoretic mobility shift assay (EMSA) by notably not changing the DNA melting temperature...
54 KB (7,076 words) - 22:19, 17 September 2024
concentrations. It has been used in the electrophoretic mobility shift assay and p50 and title comp preincubated in assay buffer, pH 7.5 (room temp., 5 min)...
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gel. It uses no charged dye so the electrophoretic mobility of proteins in CN-PAGE (in contrast to the charge shift technique BN-PAGE) is related to the...
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double-stranded (dsDNA) DNAs; however, when tested in an electrophoretic mobility shift assay, its affinity for ssDNA is significantly higher than for...
38 KB (4,760 words) - 00:26, 4 January 2024
DNA binding sites have been the DNAse footprinting assay and the Electrophoretic Mobility Shift Assay (EMSA). However, the development of DNA microarrays...
21 KB (2,176 words) - 21:02, 17 August 2024
the Bradford assay, which quantifies protein by Coomassie brilliant blue dye binding. The binding of the dye to a protein causes a shift in the absorbance...
25 KB (2,577 words) - 00:41, 22 June 2024
America former name of the Microscopy Society of America Electrophoretic mobility shift assay Emergency Medical Services Alliance, in Marion County, Florida...
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aiming to understand protein-RNA interactions included RNA Electrophoretic Mobility Shift Assays and UV-crosslinking followed by RT-PCR, however such selective...
12 KB (1,438 words) - 03:14, 15 November 2023
dodecyl sulfate–polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method...
42 KB (5,319 words) - 14:29, 2 November 2024
Crothers lab is a method for the widely used electrophoretic mobility shift assay, better known as a "gel shift" assay, which is used to detect and estimate...
12 KB (1,045 words) - 07:38, 7 November 2023
mechanism with T6SS H-NS silencing. Assays are created that combine reporter fusions, electrophoretic mobility shift assays, DNase footprinting, and fluorescence...
19 KB (2,263 words) - 01:58, 11 December 2023
site signal. A further mutation, rs208747, was shown by electrophoretic mobility shift assays to create or destroy a promoter transcription factor site...
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factors may be involved in controlling gene expression. Unlike electrophoretic mobility shift and DNA foot printing, determination of molecular weight of...
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485 – electrophoresis, starch gel MeSH E05.196.401.500 – electrophoretic mobility shift assay MeSH E05.196.401.568 – immunoelectrophoresis MeSH E05.196...
84 KB (5,386 words) - 06:43, 14 September 2024
specific cases, the detection of the phosphorylation as a shift in the protein's electrophoretic mobility is possible on simple 1-dimensional SDS-PAGE gels,...
67 KB (7,955 words) - 20:50, 29 July 2024
molecular sieving properties a high-resolution can be expected. Before an electrophoretic run is started the prepared 4% T (total polymer content (T)), 2.67%...
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E. coli during amino acid starvation. In these experiments the electrophoretic mobility of Spot42 was similar to that of 5S rRNA. In 1979 Spot42 was found...
17 KB (2,123 words) - 02:10, 18 January 2024
Through the use of immunofluorescence studies, live cell imaging, gel mobility shift assays, and bimolecular fluorescence complementation, the above was determined...
19 KB (2,572 words) - 17:45, 26 April 2024