Meganucleases are endodeoxyribonucleases characterized by a large recognition site (double-stranded DNA sequences of 12 to 40 base pairs); as a result...

I is a homing endonuclease. The enzyme is used in biotechnology as a meganuclease. It recognises an 18-base pair sequence TAGGGATAACAGGGTAAT and leaves...

transcription activator-like effector nucleases (TALENs) and engineered meganucleases—were selected by Nature Methods as the 2011 Method of the Year. The...

engineering tools, such as meganucleases. The DNA binding region of a TAL effector can be combined with the cleavage domain of a meganuclease to create a hybrid...

cell-population. Meganucleases Meganucleases are operationally analogous to ZFN's. There are limitations inherent in their use such as the meganuclease vector design...

which reduces unintended side effects. Early techniques relied on meganucleases and zinc finger nucleases. Since 2009 more accurate and easier systems...

nonhomologous end-joining. There are four families of engineered nucleases: meganucleases, zinc finger nucleases, transcription activator-like effector nucleases...

Choulika in 1999. It built up a successful business based on the use of Meganuclease in genome engineering. Cellectis became a publicly traded company in...

at specific points. There are four families of engineered nucleases: meganucleases, zinc finger nucleases, transcription activator-like effector nucleases...

at specific points. There are four families of engineered nucleases: meganucleases, zinc finger nucleases, transcription activator-like effector nucleases...

Jia; Ying, Lei; Che, Yan; Lape, Janel; Bell, Peter (September 2018). "Meganuclease targeting of PCSK9 in macaque liver leads to stable reduction in serum...

(CRISPR)-Cas9, transcription activator-like effector nucleases (TALEN), meganucleases, and zinc finger nucleases (ZFN). These tools use different mechanisms...

pairs on average (4^6=4096). One unique family of nucleases is the meganucleases, which are characterized by having larger, and therefore less common...

activator-like effector nuclease (TALEN), oligonucleotide directed mutagenesis + meganucleases. Genome editing, a type of genetic engineering Gene therapy, the therapeutic...

nuclease-based genome editing and a pioneer in the analysis and use of meganucleases to modify complex genomes. André Choulika was born in Beirut, Lebanon...

at specific points. There are four families of engineered nucleases: meganucleases, zinc finger nucleases, transcription activator-like effector nucleases...

to increase specificity and expression level is prohibited. ... ARCUS meganuclease is much smaller (~300 aa) and does not require gRNAs, making it easier...

List of homing endonuclease cutting sites I-CreI homing endonuclease Meganucleases Restriction enzyme Introns and inteins Intragenomic conflict: Homing...

cleavage domain of FokI to create TAL effector nucleases (TALEN) or to meganucleases (nucleases with longer recognition sites) to create "megaTALs." Such...

engineering Genome engineering Homing endonuclease Homologous recombination Meganuclease Zinc finger nuclease Isogenic human disease models Cas9 "Horizon Discovery...

eukaryotes it is compromised by an extremely low efficiency. Although meganucleases, zinc-finger- and transcription activator-like effector nucleases (ZFNs...

B (1992-11-11). "Nested chromosomal fragmentation in yeast using the meganuclease I-Sce I: a new method for physical mapping of eukaryotic genomes". Nucleic...