• Thumbnail for Real-time polymerase chain reaction
    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase...
    43 KB (5,338 words) - 11:09, 12 July 2024
  • Thumbnail for Reverse transcription polymerase chain reaction
    technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article...
    43 KB (5,536 words) - 07:35, 12 July 2024
  • real time PCR with the use of allele specific probes. insertions and deletions are analyzed by dosage measurements using quantitative real time PCR.[citation...
    51 KB (5,952 words) - 19:05, 6 May 2024
  • A quantitative PCR instrument is a machine that amplifies and detects DNA. It combines the functions of a thermal cycler and a fluorimeter, enabling the...
    10 KB (1,008 words) - 19:36, 1 May 2024
  • Thumbnail for Polymerase chain reaction
    qPCR is the appropriate contractions for quantitative PCR (real-time PCR). Reverse Complement PCR (RC-PCR): Allows the addition of functional domains...
    89 KB (11,117 words) - 12:33, 8 July 2024
  • PCR inhibitors or primer template mismatch. Real-time Digital PCR (rdPCR) combines the methodologies of digital PCR (dPCR) and quantitative PCR (qPCR)...
    68 KB (7,294 words) - 14:12, 27 June 2024
  • Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments...
    20 KB (2,673 words) - 11:49, 9 July 2024
  • Thumbnail for Severe combined immunodeficiency
    the U.S. are performing screening for SCID in newborns using real-time quantitative PCR to measure the concentration of T-cell receptor excision circles...
    34 KB (3,258 words) - 02:42, 29 May 2024
  • Thumbnail for MECP2 duplication syndrome
    Rocchi; Michele Zappella & Alessandra Renieri (August 2004). "Real-time quantitative PCR as a routine method for screening large rearrangements in Rett...
    9 KB (849 words) - 23:49, 7 July 2024
  • Thumbnail for Thermostable DNA polymerase
    distance between the primer and the probe in some forms of real-time quantitative PCR (qPCR). The error rates of various polymerases (fidelity) have been...
    41 KB (5,165 words) - 19:57, 29 June 2024
  • Thumbnail for Bisulfite sequencing
    "Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection". Nucleic...
    34 KB (4,084 words) - 21:59, 8 January 2024
  • hydrolysis probes that are designed to increase the specificity of quantitative PCR. The method was first reported in 1991 by researcher Kary Mullis at...
    6 KB (684 words) - 02:15, 23 December 2023
  • Thumbnail for Thermal cycler
    Thermal cycler (redirect from PCR machine)
    and cold zones on a microfluidic chip. Thermal cyclers designed for quantitative PCR have optical systems which enable fluorescence to be monitored during...
    4 KB (493 words) - 09:01, 21 May 2024
  • PMC 2642996. PMID 19240792. Abbs, S; Bobrow, M (1992). "Analysis of quantitative PCR for the diagnosis of deletion and duplication carriers in the dystrophin...
    7 KB (763 words) - 05:22, 9 June 2024
  • "Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis". Proceedings of the National...
    3 KB (334 words) - 20:53, 13 September 2023
  • Jean-Marc; La Scola, Bernard; Raoult, Didier (2012). "Comparison of real-time quantitative PCR and culture for the diagnosis of emerging Rickettsioses". PLOS...
    5 KB (454 words) - 08:27, 3 December 2023
  • Hematopoietic Necrosis Virus and White Spot Virus in Shrimp Using Real-Time Quantitative PCR and SYBR Green Chemistry". Journal of Clinical Microbiology. 39...
    16 KB (1,899 words) - 05:13, 12 March 2024
  • County in 2000, Viracor was among the first to commercially offer real-time quantitative PCR assays to diagnose patients with Adenovirus, BK virus and JC virus...
    3 KB (247 words) - 01:56, 2 November 2023
  • advantage of quantitative PCR is that analysis of resulting products using gel electrophoresis is not required. This means that quantitative PCR can be implemented...
    38 KB (5,658 words) - 00:49, 1 July 2024
  • Resolution Melting (dHRM) is also used in conjunction with digital PCR (dPCR) to improve quantitative power by providing additional information on the melting behavior...
    12 KB (1,448 words) - 07:05, 11 March 2024
  • Thumbnail for Genetically modified food controversies
    McAlexander MA, Queen SE, Adams RJ (July 2013). "Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little...
    307 KB (32,766 words) - 12:47, 7 July 2024
  • product during the amplification. Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods...
    32 KB (4,102 words) - 05:25, 7 February 2024
  • Thumbnail for COVID-19 testing
    described as real-time RT-PCR or quantitative RT-PCR and is sometimes abbreviated qRT-PCR, rRT-PCR or RT-qPCR, although sometimes RT-PCR or PCR are used....
    181 KB (21,044 words) - 08:55, 1 July 2024
  • previously limited to tracing individual transcripts by Northern blots or quantitative PCR. Higher throughput and speed allow researchers to frequently characterize...
    31 KB (3,625 words) - 09:55, 13 February 2024
  • Thumbnail for Thelohanellus kitauei
    Kim JW, Kim YC, Lee EH (2012) Molecular identification and real-time quantitative PCR (qPCR) for rapid detection of Thelohanellus kitauei, a myxozoan parasite...
    7 KB (1,036 words) - 05:39, 3 May 2023
  • expression studies, such as microarray analysis, Northern blots, or quantitative real-time PCR (qPCR). RNA that has been degraded has a direct impact on calculated...
    17 KB (2,067 words) - 01:32, 3 December 2023
  • service known as RealTimeDesign (RTD) equipped with comprehensive algorithms that allow the software to model and propose quantitative PCR and SNP genotyping...
    10 KB (999 words) - 19:07, 25 June 2024
  • Thumbnail for Lyssavirus
    reverse transcription PCR (RT-PCR) tests have been developed for rabies but only been used as a confirmatory test. Real-time PCR-based tests which have...
    18 KB (1,394 words) - 08:39, 2 July 2024
  • Harkness, W; Hayward, R; Thompson, D; Jacques, TS; et al. (2008). "Real-time quantitative PCR analysis of pediatric ependymomas identifies novel candidate genes...
    47 KB (4,967 words) - 17:39, 20 November 2023
  • Thumbnail for Sexual selection in amphibians
    CM.; DeWoody, J. (2011). "The quantification of spermatozoa by real-time quantitative PCR, spectrophotometry, and spermatophore cap size". Molecular Ecology...
    35 KB (4,203 words) - 08:16, 19 February 2024