Sephadex is a cross-linked dextran gel used for gel filtration. It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin...

(kDa) 1 Sephadex G-10 0-0.7 2 Sephadex G-25 1-5 3 Sephadex G-50 1.5-30 4 Sephadex G-75 3-70 5 Sephadex G-100 4-150 6 Sephadex G-150 5-300 7 Sephadex G-200...

Pregnenolone product can be separated from cholesterol substrate using Sephadex LH-20 minicolumns. Pregnenolone is lipophilic and readily crosses the blood–brain...

the negative charge of the substance. A slurry of resin, such as DEAE-Sephadex is poured into the column. The matrix that is used is insoluble with charged...

from a positive side group of diethylaminoethyl bound to cellulose or Sephadex. Agarose gel based medium contain large pores as well but their substitution...

of California, Berkeley Thesis Fraktionierungen von Proteinen – besonders Enzymen – mit Ionenaustauschern und Molekularsieben <vom Typ Sephadex> (1963)...

Monolayer of cell must be grown. Moreover, plastic beads of polystyrene, sephadex and polyacrylamide are also available for cell growth in suspension culture...

Styragel (cross-linked polystyrene-divinylbenzene), LH-20 (hydroxypropylated Sephadex), Bio-Gel (cross-linked polyacrylamide), HW-20 & HW-40 (hydroxylated methacrylic...

resin through lysine side chains. Gel permeation chromatography Superose Sephadex Ahmed, Hafiz (2004). Principles and Reactions of Protein Extraction, Purification...

also used in some size-exclusion chromatography matrices; an example is Sephadex. Dextran has also been used in bead form to aid in bioreactor applications...

purify cholesterol prior to use. Cholesterol can be purified using small Sephadex LH-20 columns. Cholesterol is oxidized by the liver into a variety of bile...

epichlorohydrin is its use as crosslinking agent for the production of Sephadex size-exclusion chromatographic resins from dextrans. Epichlorohydrin is...

head of research. In 1959, Pharmacia pioneered gel filtration with its Sephadex products. These were also based on dextran and discoveries in Tiselius'...

Leif; Porath, Jerker (1966). "Radioimmunoassay of proteins with the use of Sephadex-coupled antibodies". Biochimica et Biophysica Acta (BBA) - General Subjects...

was obtained by the use of ion-exchange chromatography via DEAE coated Sephadex beads. The technique separated the enzymes by the order of the corresponding...

separation in the “Y” fraction (as opposed to the “X and Z” fractions) using Sephadex G75 chromatography. As GST sub-units were identified they were referred...

for various molecular biology studies. DEAE-Sepharose, DEAE-650 and DEAE-Sephadex are commonly used in chromatography. DEAE-C is a weak anion exchanger....

"Purification of rabbit intestinal glucoamylase by affinity chromatography on Sephadex G-200". Indian J. Biochem. Biophys. 10 (4): 283–284. PMID 4792946. Sørensen...

Porath, then presented their ideas to use Pharmacia's separation medium Sephadex as the solid phase in immunodiagnostics tests and proposed a collaboration...

identified from the venom of S. mutilans. These peptides were identified by Sephadex gel filtration and RP-high-performance liquid chromatography. Both scolopin...

on 13 June 1959. A short time thereafter, Pharmacia offered the product Sephadex (Separation Pharmacia Dextran) on the market. Porath also worked on affinity...

disulphide cross-linked thiolated macromolecules within cross-linked dextran ("Sephadex") gels". Journal of Pharmacy and Pharmacology. 33 (1): 410–411. doi:10...

commonly used supporting matrix would be cross-linked agarose (sepharose), sephadex, polyacrylamide, and silica. An example for triazine linkage immobilization...

venom is dissolved in ammonium acetate buffer and then loaded on the CM-Sephadex column. The elution of the compound is done in two different steps by using...

of the apamin-competing fraction of the venom from the scorpion via a Sephadex G-50 size exclusion chromatography, followed by high performance liquid...

loop allows the injection of the desired sample. Size exclusion Sepharose Sephadex Andersson, T et al. Agarose-based media for high-resolution gel filtration...

the growth of anchorage-dependent cells, and he used diethylaminoethyl–Sephadex microcarriers. However, synthetic polymers prevent sufficient cell interactions...

toxins were then desalted and taken through four different methods of sephadex and acidic protein purification. The structure of β-Amanitin was determined...

19640680818. Fasella, Paolo; Hammes, Gordon G.; Schimmel, Paul R. (1965). "A Sephadex dialysis method of determining small molecule-macromolecule binding constants"...

potassium hydroxide (KOH) and purified by size-exclusion chromatography on Sephadex LH using DMSO–0.1%TFA. The fine-structure of the ultraviolet-visible absorption...