• Time-resolved fluorescence energy transfer (TR-FRET) is the practical combination of time-resolved fluorometry (TRF) with Förster resonance energy transfer...
    10 KB (1,293 words) - 01:37, 25 July 2022
  • Thumbnail for Förster resonance energy transfer
    Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism...
    52 KB (6,185 words) - 03:43, 30 October 2024
  • and emissive states. Time-resolved fluorescence spectroscopy is an extension of fluorescence spectroscopy. Here, the fluorescence of a sample is monitored...
    13 KB (1,687 words) - 17:19, 15 December 2023
  • Thumbnail for Fluorescence spectroscopy
    Devices that measure fluorescence are called fluorometers. Molecules have various states referred to as energy levels. Fluorescence spectroscopy is primarily...
    22 KB (2,841 words) - 16:12, 30 April 2024
  • Thumbnail for Quenching (fluorescence)
    for fluorescence microscopy, or can be harnessed in sensors of proteolysis. There are a few distinct mechanisms by which energy can be transferred non-radiatively...
    9 KB (1,023 words) - 08:01, 6 May 2024
  • Thumbnail for Plate reader
    screening applications, under a form called TR-FRET (time-resolved fluorescence energy transfer). TR-FRET assays are very robust (limited sensitivity...
    15 KB (1,944 words) - 17:02, 30 August 2024
  • fluorescence, a molecule absorbs a photon and gets excited to a higher energy state. After a short delay (the average represented as the fluorescence...
    14 KB (2,049 words) - 21:37, 6 November 2024
  • fluorophores is excited by an ultrashort or delta pulse of light, the time-resolved fluorescence will decay exponentially as described above. However, if the excitation...
    24 KB (3,092 words) - 21:09, 11 July 2024
  • Thermally activated delayed fluorescence (TADF) is a process through which surrounding thermal energy changes population of excited states of molecular...
    21 KB (2,494 words) - 03:21, 18 November 2024
  • /\nu } laser fluorescence spectroscopy on Tl+ mono-ion oscillator III (sideband cooling).’’ The clarification is important as at the time of the latter...
    19 KB (2,511 words) - 19:08, 21 November 2024
  • Fluorescence correlation spectroscopy (FCS) is a statistical analysis, via time correlation, of stationary fluctuations of the fluorescence intensity...
    63 KB (7,969 words) - 14:18, 17 October 2024
  • Stacking Interactions in Flavin Adenine Dinucleotide from Time-Resolved Flavin Fluorescence". Photochemistry and Photobiology. 40 (6): 703–706. doi:10...
    33 KB (4,369 words) - 11:23, 24 November 2024
  • Thumbnail for Fluorescence imaging
    light. Fluorescence is the physical excitation of an electron, and subsequent return to emit light. When a certain molecule absorbs light, the energy of the...
    15 KB (1,905 words) - 16:52, 25 September 2024
  • Bimolecular fluorescence complementation BKD – Backscatter Kikuchi diffraction, see EBSD BRET – Bioluminescence resonance energy transfer BSED – Back...
    15 KB (1,398 words) - 20:21, 9 November 2024
  • record the fluorescence emission at different decay times from the given excitation energy at time zero. This is called time resolved fluorescence spectroscopy...
    19 KB (2,421 words) - 16:47, 5 March 2024
  • Thumbnail for Protein folding
    Potential energy of protein Protein dynamics Protein misfolding cyclic amplification Protein structure prediction software Proteopathy Time-resolved mass spectrometry...
    76 KB (8,728 words) - 11:37, 6 November 2024
  • applications. Ultrafast and time-resolved spectroscopic techniques, including transient absorption and time-resolved fluorescence spectroscopy, allows determination...
    11 KB (1,266 words) - 03:36, 18 November 2024
  • limitations of traditional wide-field fluorescence microscopes. In a conventional (i.e., wide-field) fluorescence microscope, the entire specimen is flooded...
    46 KB (5,308 words) - 02:06, 5 November 2024
  • Thumbnail for Photoluminescence
    to as fluorescence spectroscopy, but the instrumentation is the same. The relaxation processes can be studied using time-resolved fluorescence spectroscopy...
    27 KB (3,340 words) - 07:43, 20 March 2024
  • Single-molecule fluorescence (or Förster) resonance energy transfer (or smFRET) is a biophysical technique used to measure distances at the 1-10 nanometer...
    45 KB (6,137 words) - 15:28, 21 October 2024
  • Thumbnail for Resonance Raman spectroscopy
    widths than fluorescence. However, fluorescence and resonance Raman scattering co-occur in many materials, and interference from fluorescence may complicate...
    24 KB (2,772 words) - 22:14, 25 November 2024
  • additional property of fluorescence that has found a large usage is Förster resonance energy transfer (Fret) in which the energy of the excited electron...
    54 KB (7,448 words) - 20:18, 3 May 2022
  • visualization at first in the electron microscope and, more recently, with fluorescence microscopy. Over the past two decades, a new generation of characterization...
    20 KB (2,764 words) - 23:32, 24 October 2024
  • Thumbnail for Two-photon absorption
    two-photon-excited fluorescence where the excited state produced by two-photon absorption decays by spontaneous emission of a photon to a lower energy state. Two-photon...
    38 KB (4,756 words) - 09:13, 25 November 2024
  • Thumbnail for Charge-coupled device
    capacitors. Under the control of an external circuit, each capacitor can transfer its electric charge to a neighboring capacitor. CCD sensors are a major...
    50 KB (6,374 words) - 13:15, 18 November 2024
  • Thumbnail for Spectroscopy
    Spectroscopy (category Scattering, absorption and radiative transfer (optics))
    induced by other sources of energy such as flames, sparks, electric arcs or electromagnetic radiation in the case of fluorescence. Elastic scattering and...
    42 KB (4,615 words) - 16:25, 1 November 2024
  • effects and provide ultra-fast recording of FCCS or FCS data. Fluorescence resonance energy transfer (FRET) is a common technique when observing the interactions...
    35 KB (4,515 words) - 07:57, 6 August 2024
  • Thumbnail for Quantum dot
    Ulrich J. (7 November 2007). "Quantum dots as donors in fluorescence resonance energy transfer for the bioanalysis of nucleic acids, proteins, and other...
    113 KB (13,222 words) - 13:44, 14 November 2024
  • Thumbnail for Nicotinamide adenine dinucleotide
    PMC 48431. PMID 1741380. Jameson DM, Thomas V, Zhou DM (1989). "Time-resolved fluorescence studies on NADH bound to mitochondrial malate dehydrogenase"....
    79 KB (9,020 words) - 21:04, 28 October 2024
  • Thumbnail for Equipartition theorem
    as fluorescence anisotropy, flow birefringence and dielectric spectroscopy. Equipartition applies to potential energies as well as kinetic energies: important...
    90 KB (11,949 words) - 05:10, 19 June 2024