Time-resolved fluorescence energy transfer (TR-FRET) is the practical combination of time-resolved fluorometry (TRF) with Förster resonance energy transfer...
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Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism...
52 KB (6,185 words) - 03:43, 30 October 2024
and emissive states. Time-resolved fluorescence spectroscopy is an extension of fluorescence spectroscopy. Here, the fluorescence of a sample is monitored...
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Devices that measure fluorescence are called fluorometers. Molecules have various states referred to as energy levels. Fluorescence spectroscopy is primarily...
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for fluorescence microscopy, or can be harnessed in sensors of proteolysis. There are a few distinct mechanisms by which energy can be transferred non-radiatively...
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Plate reader (section Time-resolved fluorescence (TRF))
screening applications, under a form called TR-FRET (time-resolved fluorescence energy transfer). TR-FRET assays are very robust (limited sensitivity...
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fluorescence, a molecule absorbs a photon and gets excited to a higher energy state. After a short delay (the average represented as the fluorescence...
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fluorophores is excited by an ultrashort or delta pulse of light, the time-resolved fluorescence will decay exponentially as described above. However, if the excitation...
24 KB (3,092 words) - 21:09, 11 July 2024
Thermally activated delayed fluorescence (TADF) is a process through which surrounding thermal energy changes population of excited states of molecular...
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/\nu } laser fluorescence spectroscopy on Tl+ mono-ion oscillator III (sideband cooling).’’ The clarification is important as at the time of the latter...
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Fluorescence correlation spectroscopy (FCS) is a statistical analysis, via time correlation, of stationary fluctuations of the fluorescence intensity...
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Ultrafast laser spectroscopy (redirect from Time-correlated single photon counting)
Stacking Interactions in Flavin Adenine Dinucleotide from Time-Resolved Flavin Fluorescence". Photochemistry and Photobiology. 40 (6): 703–706. doi:10...
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light. Fluorescence is the physical excitation of an electron, and subsequent return to emit light. When a certain molecule absorbs light, the energy of the...
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Bimolecular fluorescence complementation BKD – Backscatter Kikuchi diffraction, see EBSD BRET – Bioluminescence resonance energy transfer BSED – Back...
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record the fluorescence emission at different decay times from the given excitation energy at time zero. This is called time resolved fluorescence spectroscopy...
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Protein folding (section Fluorescence spectroscopy)
Potential energy of protein Protein dynamics Protein misfolding cyclic amplification Protein structure prediction software Proteopathy Time-resolved mass spectrometry...
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applications. Ultrafast and time-resolved spectroscopic techniques, including transient absorption and time-resolved fluorescence spectroscopy, allows determination...
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Confocal microscopy (redirect from X-Ray Fluorescence Imaging)
limitations of traditional wide-field fluorescence microscopes. In a conventional (i.e., wide-field) fluorescence microscope, the entire specimen is flooded...
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to as fluorescence spectroscopy, but the instrumentation is the same. The relaxation processes can be studied using time-resolved fluorescence spectroscopy...
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Single-molecule fluorescence (or Förster) resonance energy transfer (or smFRET) is a biophysical technique used to measure distances at the 1-10 nanometer...
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widths than fluorescence. However, fluorescence and resonance Raman scattering co-occur in many materials, and interference from fluorescence may complicate...
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additional property of fluorescence that has found a large usage is Förster resonance energy transfer (Fret) in which the energy of the excited electron...
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visualization at first in the electron microscope and, more recently, with fluorescence microscopy. Over the past two decades, a new generation of characterization...
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two-photon-excited fluorescence where the excited state produced by two-photon absorption decays by spontaneous emission of a photon to a lower energy state. Two-photon...
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Charge-coupled device (redirect from Frame transfer CCD)
capacitors. Under the control of an external circuit, each capacitor can transfer its electric charge to a neighboring capacitor. CCD sensors are a major...
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Spectroscopy (category Scattering, absorption and radiative transfer (optics))
induced by other sources of energy such as flames, sparks, electric arcs or electromagnetic radiation in the case of fluorescence. Elastic scattering and...
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effects and provide ultra-fast recording of FCCS or FCS data. Fluorescence resonance energy transfer (FRET) is a common technique when observing the interactions...
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Ulrich J. (7 November 2007). "Quantum dots as donors in fluorescence resonance energy transfer for the bioanalysis of nucleic acids, proteins, and other...
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PMC 48431. PMID 1741380. Jameson DM, Thomas V, Zhou DM (1989). "Time-resolved fluorescence studies on NADH bound to mitochondrial malate dehydrogenase"....
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Equipartition theorem (redirect from Equipartition of energy)
as fluorescence anisotropy, flow birefringence and dielectric spectroscopy. Equipartition applies to potential energies as well as kinetic energies: important...
90 KB (11,949 words) - 05:10, 19 June 2024