CRISPR activation (CRISPRa) is a type of CRISPR tool that uses modified versions of CRISPR effectors without endonuclease activity, with added transcriptional...
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debate. CRISPR activation Anti-CRISPR CRISPR/Cas Tools CRISPR gene editing The CRISPR Journal "Designer baby" DRACO Gene knockout Genome-wide CRISPR-Cas9...
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CRISPR gene editing (CRISPR, pronounced /ˈkrɪspər/ "crisper", refers to "clustered regularly interspaced short palindromic repeats") is a genetic engineering...
170 KB (19,936 words) - 22:49, 27 August 2024
CRISPR-Display (CRISP-Disp) is a modification of the CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) system for genome editing...
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Sequence-specific activation of gene expression refers to CRISPR activation (CRISPRa). Based on the bacterial genetic immune system - CRISPR (clustered regularly...
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Cas9 (redirect from CRISPR/Cas9)
Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense...
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Anti-CRISPR (Anti-Clustered Regularly Interspaced Short Palindromic Repeats or Acr) is a group of proteins found in phages, that inhibit the normal activity...
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In molecular biology, trans-activating CRISPR RNA (tracrRNA) is a small trans-encoded RNA. It was first discovered by Emmanuelle Charpentier in her study...
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CRISPR RNA or crRNA is a RNA transcript from the CRISPR locus. CRISPR-Cas (clustered, regularly interspaced short palindromic repeats - CRISPR associated...
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Genome-wide CRISPR-Cas9 knockout screens aim to elucidate the relationship between genotype and phenotype by ablating gene expression on a genome-wide...
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RNA polymerase that recruits RNA polymerase to the lac promoter. CRISPR activation Bacterial transcription Coactivator (genetics) Eukaryotic transcription...
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breaks requiring activation of host repair mechanisms. They also lack Cas1 and Cas2 proteins and so rely on other more complete CRISPR systems for spacer...
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editing with engineered nucleases. Alongside zinc finger nucleases and CRISPR/Cas9, TALEN is a prominent tool in the field of genome editing. TAL effectors...
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Guide RNA (section CRISPR In Prokaryotes)
used for gene editing. In bacteria and archaea, gRNAs are a part of the CRISPR-Cas system that serves as an adaptive immune defense that protects the organism...
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Epigenome editing (section CRISPR-Cas)
Zinc finger proteins, Transcription Activator-Like Effectors (TALEs) and nuclease deficient Cas9 fusions (CRISPR). Comparing genome-wide epigenetic maps...
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infection, CTL activation is tightly controlled and in general requires a very strong MHC/antigen activation signal, or additional activation signals provided...
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Designer baby (section CRISPR/Cas9)
homology directed repair. The CRISPR/Cas9 system (CRISPR – Clustered Regularly Interspaced Short Palindromic Repeats, Cas9 – CRISPR-associated protein 9) is...
71 KB (8,210 words) - 04:47, 10 July 2024
on pooled genetic perturbation screens. Perturb-seq combines multiplexed CRISPR mediated gene inactivations with single cell RNA sequencing to assess comprehensive...
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Genome editing (section CRISPR)
transcription activator-like effector nucleases (TALENs) and engineered meganucleases—were selected by Nature Methods as the 2011 Method of the Year. The CRISPR-Cas...
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Gene knockdown (section CRISPRs)
or CRISPRs. CRISPR-associated (cas) genes encode cellular machinery that cuts exogenous DNA into small fragments and inserts them into a CRISPR repeat...
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clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9, transcription activator-like effector nucleases (TALEN), meganucleases, and zinc...
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S phases in the cell cycle. CRISPR gene editing based on Clustered regularly interspaced short palindromic repeats (CRISPR) -Cas9 is an enzyme that uses...
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trial using CRISPR-Cas9 gene editing of T cells in patients with refractory cancer demonstrates that, according to their study, such CRISPR-based therapies...
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Human genetic enhancement (section CRISPR/Cas9)
benefits of revolutionary tools like CRISPR are endless. For example, because it can be applied directly in the embryo, CRISPR/Cas9 reduces the time required...
95 KB (10,491 words) - 00:01, 9 August 2024
Gene knockout (section CRISPR/Cas9)
done through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs. One of the main advantages of gene knockouts is that they...
21 KB (2,524 words) - 18:03, 29 April 2024
Functional genomics (section CRISPR screens)
Whitehead EH, et al. (October 2014). "Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation". Cell. 159 (3): 647–61. doi:10.1016/j.cell.2014...
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ISSN 0002-9289. PMID 1781479. The-Crispr (2019-05-13). "Antidote to deadly jellyfish identified using CRISPR". The Crispr. Retrieved 2019-08-08. Neely, G...
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brother used a CRISPR transcriptional activator which functions to upregulate an alternative isoform of dystrophin. Because the CRISPR activation technology...
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obtain desired results. Examples of gene editing are CRISPR, zinc finger nuclease, transcription activator-like effector nuclease (TALEN), oligonucleotide...
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Germline mutation (section CRISPR/Cas9 editing)
initiates cellular repair processes, similar to that of CRISPR/Cas9 DNA editing. Compared to CRISPR/Cas9, the therapeutic applications of this technology...
25 KB (3,085 words) - 14:19, 5 August 2024