• Thumbnail for Aminoallyl nucleotide
    Aminoallyl nucleotide is a nucleotide with a modified base containing an allylamine. They are used in post-labeling of nucleic acids by fluorescence detection...
    13 KB (1,413 words) - 15:01, 8 January 2024
  • Thumbnail for Nucleotide base
    used as fluorescent probes, either directly or indirectly, such as aminoallyl nucleotide, which are used to label cRNA or cDNA in microarrays. Several groups...
    15 KB (1,434 words) - 17:28, 1 July 2024
  • the coupling stage occurs before hybridization, using aminoallyl uridine triphosphate (aminoallyl-UTP, or aaUTP) and NHS amino-reactive dyes (such as cyanine...
    54 KB (5,427 words) - 14:19, 26 April 2024
  • Thumbnail for Nucleic acid analogue
    with a reactive fluorophore (indirect labelling): Amine reactive: aminoallyl nucleotides contain a primary amine group on a linker that reacts with the amino-reactive...
    49 KB (5,301 words) - 01:33, 26 June 2024
  • nucleic acids lack, nucleotides have to be modified with aminoallyl groups. This is done through incorporating aminoallyl-modified nucleotides during synthesis...
    17 KB (1,827 words) - 07:44, 7 June 2024
  • Thumbnail for Fluorescent in situ sequencing
    packed into a short string of DNA, as short as 15-20 nucleotides long for the mouse brain or 5 nucleotides for targeted cancer gene panels. In FISSEQ, a series...
    6 KB (734 words) - 20:10, 1 November 2023