Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' because...

electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. After first being...

(LNA), morpholinos and peptide nucleic acids (PNA). In sequencing, dideoxynucleotides are used. These nucleotides possess the non-canonical sugar dideoxyribose...

labeled-primer approach. This is accomplished by labelling each of the dideoxynucleotide chain-terminators with a separate fluorescent dye, which fluoresces...

Hybridization is followed by single-base extension with hapten-labeled dideoxynucleotides. The ddCTP and ddGTP are labeled with biotin while ddATP and ddUTP...

which is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. Recently, shotgun...

which used a novel fluorescent labeling technique enabling all four dideoxynucleotides to be identified in a single lane. By 1990, the U.S. National Institutes...

"Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation". Proceedings of the National Academy of Sciences of...

or, for PNA, other methods of peptide synthesis.[citation needed] Dideoxynucleotides are used in sequencing. These nucleoside triphosphates possess a non-canonical...

polymerase primed synthesis ("plus and minus") technique and, eventually, dideoxynucleotide chain-terminating sequencing, which was later termed Sanger sequencing...

Alan Coulson presented a rapid gene sequencing technique which uses dideoxynucleotides and gel electrophoresis. 1978 – Frederick Sanger presented the 5,386...

to or greater than that from the Sanger sequencing method based on dideoxynucleotide chain termination. The longer read length allows de novo genome sequencing...

cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased...

approaches which use the incorporation of either fluorescently labeled dideoxynucleotides (ddNTP) or fluorescently labeled deoxynucleotides (dNTP). With ddNTPs...

template sequence, during which fluorochrome-labeled, chain-terminating dideoxynucleotides are randomly incorporated in the elongating strand; the resulting...

methylation analysis using bisulfite-treated DNA utilized PCR and standard dideoxynucleotide DNA sequencing to directly determine the nucleotides resistant to...

terminal transferase or ‘tailing’ activity' to add a chain-terminating dideoxynucleotide base, ddRTP (ddATP or ddGTP), to the 3’ end of the IT, in the presence...

polymerase can also be used for the incorporation of fluorescence-labelled dideoxynucleotides. The template specificity of the polymerases is increased by using...

nucleotides, which accounts for the sensitivity of T7 polymerase to dideoxynucleotides (see Sequenase below). The primary gp5 subunit of T7 DNA Polymerase...