The two most established forms of gene editing are gene-targeting and targeted-mutagenesis. While gene targeting relies on the Homology Directed Repair...

Targeting may refer to: Gene targeting Protein targeting Behavioral targeting Targeted advertising Target market Geotargeting, in internet marketing Targeting...

well as reduced off target effects. This could be used for research purposes, by targeting mutations to specific genes, and in gene therapy. By inserting...

Gene knockouts (also known as gene deletion or gene inactivation) are a widely used genetic engineering technique that involves the targeted removal or...

types of molecular genes: protein-coding genes and non-coding genes. During gene expression (the synthesis of RNA or protein from a gene), DNA is first copied...

used for gene targeting to make a chimera, the following procedure is common: a construct for homologous recombination for the gene targeted will be introduced...

Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. For their development of this...

while having an increased ability to avoid degradation by target cell lysosomes. Gene targeting Minicircle Plasmid Transgene Vector (molecular biology)...

targeted to a specific location. The technique of gene targeting uses homologous recombination to make desired changes to a specific endogenous gene....

modulate gene expression by targeting dCas9-repressors or activators toward promoter or transcriptional start sites of target genes. For repressing genes Cas9...

exist for integration. To create a stable mutant line, gene targeting can be used to insert a gene of interest into a particular location of the C. merolae...

of early and late genes. Silencing of IE gene expression through antisense oligonucleotides, RNA interference, and gene-targeting ribosomes have been...

that a knock-in involves a gene inserted into a specific locus, and is thus a "targeted" insertion. It is the opposite of gene knockout. A common use of...

recombination could be used for conditional gene targeting. They observed ≈50% of the DNA polymerase beta gene was deleted in T cells based on DNA blotting...

the virus family Caliciviridae, by targeting both its structural and non-structural genes. By targeting the NTPase gene, one dose of siRNA 4 hours pre-infection...

specimens. Early techniques randomly inserted the genes into the genome. Advances allow targeting specific locations, which reduces unintended side effects...

c-myc gene by one of three chromosomal translocations which place the myc gene under the control of an immunoglobulin gene enhancer. The MYC gene is found...

cell-specific targeting and subcellular trafficking control. Methods for non-viral gene therapy include the injection of naked DNA, electroporation, the gene gun...

the loop to target genes outside the loop. Loops with CTCF and cohesin at the start and end of the loop that restrict enhancer-gene targeting are "insulated...

the role of individual genes in living organisms. It differs from traditional gene knockout because it targets specific genes at specific times rather...

increasing their levels of targeted HR while still maintain their reduced NHEJ frequency. Chimeric nuclease Genome editing Gene targeting Zinc finger protein...

P, Xiao A, Zhou M, Zhu Z, Lin S, Zhang B (August 2011). "Heritable gene targeting in zebrafish using customized TALENs". Nature Biotechnology. 29 (8):...

altering animal genomes than previously used, and the technique behind gene targeting and knockout mice. He received the Nobel Prize in Physiology or Medicine...

Talk: "The Birth of Gene Targeting" Mario R. Capecchi on Nobelprize.org including the Nobel Lecture on 7 December 2007 Gene Targeting 1977 - Present Eccles...

RNA-induced gene activation" or RNAa. It has been shown that dsRNAs targeting gene promoters induce potent transcriptional activation of associated genes. RNAa...

Xiang, Andy Peng (2014). "Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos". Cell. 156 (4): 836–843...

mouse and the related technology of gene targeting, a method of using embryonic stem cells to create specific gene modifications in mice. In 2007, the...

Creating Knockout Mice for Targeting Vector from Knockout Mice Research(KMR) – A website for ordering embryonic stem cells, targeting vectors and transgenic...

that an exogenous DNA sequence can be targeted to a specific genomic position (a technique called gene targeting) to create knockout mosses. This approach...

into the germline. They also allow precise genetic modifications by gene targeting. Modified embryonic stem cells can be selected in vitro before the experiment...