The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by...

The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...

including unidirectional ligation or concatemerization, overlap extension polymerase chain reaction (OEPCR), and recursive directional ligation (RDL). Also...

many modern televisions Splicing by overlap extension polymerase chain reaction, a variant of polymerase chain reaction Standard Operating Environment, computer...

The versatility of polymerase chain reaction (PCR) has led to modifications of the basic protocol being used in a large number of variant techniques designed...

electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. After first being...

A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists...

at known sequences in a template DNA molecule. Polymerase chain reaction (PCR), ligase chain reaction (LCR), and transcription-mediated amplification...

Repair Cloning is a particular form of the polymerase chain reaction in which specialized primers with extensions beyond the sequence of the DNA target are...

restriction enzymes or integrases. Other similar overlap-based assembly methods include Circular Polymerase Extension Cloning (CPEC), Sequence and Ligase Independent...

manipulate it in the laboratory, such as restriction digests and the polymerase chain reaction. Modern biology and biochemistry make intensive use of these techniques...

isolation. The number of copies of the gene is then amplified using polymerase chain reaction (PCR). Simultaneously, the same restriction enzymes are used to...

Repair Cloning is a particular form of the Polymerase Chain Reaction in which specialized primers with extensions beyond the sequence of the DNA target are...

concentration of Taq polymerase. Increase extension time. Increase cycle time. Use less accurate Taq polymerase. Also see polymerase chain reaction for more information...

cDNA molecule is then synthesized through a process called PCR (Polymerase Chain Reaction), which amplifies the cDNA to produce multiple copies. 3)Sequencing:...

Vectorette PCR is a variation of polymerase chain reaction (PCR) designed in 1988. The original PCR was created and also patented during the 1980s. Vectorette...

chain-termination method or 'Sanger method', which is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during...

After melting, the reaction is immediately quenched to 0 °C, and MALBAC primers are added to the reaction. Extension – Bst DNA Polymerase (Large Fragment)...

are milled to a powder and treated with a solution before the polymerase chain reaction (PCR) process. Samples for DNA amplification may not necessarily...

specialized polymerases to bypass the lesion and resume DNA replication. After translesion synthesis, extension is required. This extension can be carried...

more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication...

fragments with sufficiently overlapping sequences will anneal to each other and then be extended by DNA polymerase. The PCR extension will not occur unless...

in the parasite genome. The advancement in technologies like polymerase chain reaction (PCR) led to the development of various assays that made large...

amplification reaction. The term is most frequently used to describe the numerous copied fragments that are the products of the polymerase chain reaction or ligase...

molecular diagnostics methods based on nucleic acids detection use polymerase chain reaction (PCR) to vastly increase the number of nucleic acid molecules...

senescence or biological aging, including the research and development of life extension methods, brain aging delay methods and rejuvenation. People have long...

polymer A large macromolecule composed of many repeated subunits. polymerase chain reaction (PCR) A technique used in molecular biology to amplify a single...

traits largely, but not completely, overlap the classifications based on color, ATPase, or MHC (myosin heavy chain). Some authors define a fast twitch...

characteristics. Around the same time the development of effective polymerase chain reaction techniques made it possible to apply cladistic methods of analysis...

genome surveillance complex (BASC). The BRCA1 protein associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase...