molecular biology, Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium...
10 KB (1,063 words) - 20:45, 11 September 2024
Serine protease (redirect from Serine proteinase inhibitors)
MEROPS - Database of protease evolutionary groups Keratinase Subtilisin Proteinase K Hedstrom L (December 2002). "Serine protease mechanism and specificity"...
22 KB (2,343 words) - 20:13, 29 August 2024
to the protein's structure or function. PrPC is readily digested by proteinase K and can be liberated from the cell surface by the enzyme phosphoinositide...
99 KB (10,732 words) - 13:28, 2 November 2024
carbohydrates, proteins, and extracellular DNA. Detachment assays with NaIO4, proteinase K, or DNase result in 38%, 98%, and 100% detachment, respectively. The...
26 KB (2,402 words) - 19:27, 28 November 2023
activity or removal of unwanted proteins. For example, proteinase K, a broad-spectrum proteinase stable in urea and SDS, is often used in the preparation...
34 KB (4,247 words) - 21:28, 2 November 2024
Proteinase K has a catalytic triad of Ser 224, His 69, and Asp 39. Two peptide chains recognize substrates, 99-104 and 132–136. There is also a cysteine...
2 KB (269 words) - 01:56, 26 January 2023
separated from free RNA using gel electrophoresis and membrane transfer. Proteinase K digestion is then performed in order to remove protein from the crosslinked...
50 KB (4,888 words) - 11:50, 22 February 2024
Caricain (redirect from Papaya proteinase)
papaya peptidase II, papaya proteinase, papaya proteinase III, papaya proteinase 3, proteinase omega, papaya proteinase A, chymopapain S, Pp) is an enzyme...
15 KB (2,014 words) - 14:15, 8 November 2023
detection of prion-infected material in whole human blood without the use of proteinase K" (PDF). Transfusion (Submitted manuscript). 50 (12): 2619–27. doi:10...
70 KB (7,661 words) - 01:26, 20 October 2024
fluorescence or brightfield microscope. permeabilization of cells with proteinase K to open cell membranes (around 25 minutes, not needed for tissue sections...
12 KB (1,319 words) - 10:37, 9 July 2024
termination. First, genomic DNA (gDNA) is extracted from cells of interest by proteinase K treatment followed by phenol-chloroform extraction and ethanol precipitation...
16 KB (1,997 words) - 09:10, 5 August 2024
used to break open cells and release the DNA. Protease enzymes, such as Proteinase K, which are used to digest proteins that may be binding to the DNA. Phenol...
24 KB (3,183 words) - 18:51, 17 October 2024
RNA unusable. To remove DNA and proteins, enzymes such as DNase and Proteinase K are used for degradation. Importantly, RNA integrity is maintained by...
19 KB (2,275 words) - 03:38, 13 October 2024
can also degrade PLA. Enzymes such as pronase and most effectively proteinase K from Tritirachium album degrade PLA. Four possible end-of-life scenarios...
45 KB (5,165 words) - 18:14, 5 November 2024
Aspergillopepsin I (redirect from Aspergillus acid proteinase)
Aspergillus acid proteinase, Aspergillus aspartic proteinase, Aspergillus awamori acid proteinase, Aspergillus carboxyl proteinase, carboxyl proteinase, Aspergillus...
5 KB (499 words) - 13:15, 26 August 2023
Protease (redirect from Proteinase)
A protease (also called a peptidase, proteinase, or proteolytic enzyme) is an enzyme that catalyzes proteolysis, breaking down proteins into smaller polypeptides...
26 KB (2,799 words) - 15:02, 30 June 2024
improve image quality in gastroscopy by thinning the mucus in advance. Proteinase K Teh, Jun-Liang; Shabbir, Asim; Yuen, Soon; So, Jimmy Bok-Yan (2020)....
1 KB (131 words) - 15:03, 1 November 2024
sections before the advent of AR. In enzyme digestion, enzymes such as proteinase K, trypsin, and pepsin are used to restore antibody binding to its epitope...
10 KB (1,183 words) - 14:32, 27 July 2024
Tryptase (redirect from Mast cell neutral proteinase)
(EC 3.4.21.59) is the most abundant secretory granule-derived serine proteinase contained in mast cells and has been used as a marker for mast cell activation...
8 KB (836 words) - 11:44, 16 October 2024
Oryzin (redirect from Aspergillus alkaline proteinase)
alkaline proteinase, Aspergillus melleus semi-alkaline proteinase, Aspergillus oryzae alkaline proteinase, Aspergillus parasiticus alkaline proteinase, Aspergillus...
3 KB (320 words) - 21:50, 21 June 2024
biocon.2014.11.019. Tsuji, Satsuki (2016). "Effects of water pH and proteinase K treatment on the yield of environmental DNA from water samples". Limnology...
131 KB (13,421 words) - 05:16, 9 October 2024
Kexin (redirect from Proteinase yscF)
gene KEX2 dibasic proteinase, Kex 2p proteinase, Kex2 endopeptidase, Kex2 endoprotease, Kex2 endoproteinase, Kex2 protease, proteinase Kex2p, Kex2-like...
5 KB (504 words) - 22:17, 1 September 2023
protein–DNA cross-link is reversed and proteins are removed by digestion with proteinase K. An epitope-tagged version of the protein of interest, or in vivo biotinylation...
25 KB (2,929 words) - 08:17, 12 July 2024
Sreekumar K, Purandare U, Lo CF, Huang Y, et al. (December 2003). "Functional characterization of Narc 1, a novel proteinase related to proteinase K". Archives...
69 KB (7,354 words) - 16:56, 11 June 2024
proteins bound to the DNA have to be detached and degraded. First, Proteinase K, sodium dodecyl sulfate (SDS; a detergent), Tween-20, and nuclease-free...
17 KB (1,988 words) - 08:07, 2 June 2024
Serralysin (redirect from Pseudomonas aeruginosa alkaline proteinase)
Pseudomonas aeruginosa alkaline proteinase, Escherichia freundii proteinase, Serratia marcescens extracellular proteinase, Serratia marcescens metalloproteinase...
4 KB (396 words) - 00:15, 28 October 2024
Papain (redirect from Papaya proteinase I)
Papain, also known as papaya proteinase I, is a cysteine protease (EC 3.4.22.2) enzyme present in papaya (Carica papaya) and mountain papaya (Vasconcellea...
18 KB (2,006 words) - 23:38, 27 October 2024
proteolytic enzymes that digest keratin. They were initially classified as 'proteinases of unknown mechanism' by the Nomenculture Committee on the International...
9 KB (1,010 words) - 05:15, 19 September 2024
(a protease) cleaves VPg from membrane-bound 3AB. Studies that used proteinase K to cleave VPg from the viral genome discovered that calicivirus vesicular...
5 KB (731 words) - 00:43, 24 July 2024
Alpha-1 antitrypsin (redirect from Alpha-1-proteinase inhibitor)
by the SERPINA1 gene. A protease inhibitor, it is also known as alpha1–proteinase inhibitor (A1PI) or alpha1-antiproteinase (A1AP) because it inhibits various...
33 KB (3,383 words) - 08:00, 21 August 2024