Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I-like mechanism to carry out site...

Recombinases are genetic recombination enzymes. DNA recombinases are widely used in multicellular organisms to manipulate the structure of genomes, and...

sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 μ plasmid of baker's yeast Saccharomyces...

Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites...

Recombinase polymerase amplification (RPA) is a single tube, isothermal alternative to the polymerase chain reaction (PCR). By adding a reverse transcriptase...

Site-specific recombinase technologies are genome engineering tools that depend on recombinase enzymes to replace targeted sections of DNA. In the late...

majority of T cells. The process of V(D)J recombination is mediated by VDJ recombinase, which is a diverse collection of enzymes. The key enzymes involved are...

RMCE (recombinase-mediated cassette exchange) is a procedure in reverse genetics allowing the systematic, repeated modification of higher eukaryotic genomes...

recombination. Recombination between LoxP sites is catalysed by Cre recombinase. The term "floxing" is a portmanteau constructed from the phrase "flanking/flanked...

certain degree of sequence homology. Enzymes known as site-specific recombinases (SSRs) perform rearrangements of DNA segments by recognizing and binding...

DNA integration, by means of site-specific recombinase technology, performed by a specific class of recombinase enzymes ("integrases") Economic integration...

Hin recombinase is a 21kD protein composed of 198 amino acids that is found in the bacteria Salmonella. Hin belongs to the serine recombinase family (B2)...

RadB and RadC recombinase paralogs are found in many organisms belonging to Euryarchaeota, while a broader diversity of related recombinase paralogs are...

Tre recombinase is an experimental enzyme that in lab tests has removed DNA inserted by HIV from infected cells. Through selective mutation, Cre recombinase...

meiosis-specific recombinase Dmc1 and the general recombinase Rad51 coat the single-stranded DNA to form nucleoprotein filaments. The recombinases catalyze invasion...

recombination (HR) ordinarily occurs during meiosis. The meiosis-specific recombinase, Dmc1, is required for efficient meiotic HR, and Dmc1 is expressed in...

enzymes involved in spermatogenesis, spermatogenic DNA polymerase beta and recombinase activities evolving a unique temperature optimum that is slightly less...

abnormalities. The recombination reaction is catalyzed by enzymes known as recombinases, such as RAD51. The first step in recombination is a double-stranded...

engineering. These genetically engineered mouse models typically use a Cre recombinase system to disrupt tumor suppressors or activate oncogenes specifically...

body temperature in most of mammals, as β-polimerase and spermatogenic recombinase need a specific optimal temperature. Consequently, the testes are located...

with an aspartic acid residue in order to disable the activity of the recombinases and inactivate homologous recombination. The endA1 mutation inactivates...

commonly used technique is the Cre-lox recombination system. The Cre recombinase enzyme specifically recognizes two lox (loci of recombination) sites...

Recombination in bacteria is ordinarily catalyzed by a RecA type of recombinase. These recombinases promote repair of DNA damages by homologous recombination....

recombinase called Cre, the original conditional knockout technique recombined short target sequences known as LoxP. Since then, other recombinases have...

that enable transgenic expression of FRT recombination sites and a Flp recombinase in non-human mammals. Her technology has aided many researchers in achieving...

family of tyrosine recombinases. The interaction of Xer D and the γ subunit of FtsKC results in the activation of the recombinase. Contact between Xer...

as psm-mecRNA. SCCmec also contains ccrA and ccrB; both genes encode recombinases that mediate the site-specific integration and excision of the SCCmec...

station based in Biloxi, Mississippi Cre-Lox recombination a site-specific recombinase technology widely used to carry out deletions, insertions, translocations...

flexible. The flip recombinase (or FLP) is a gene from the commonly studied yeast Saccharomyces cerevisiae that recognizes "flip recombinase target" (FRT)...

different enzymes. Recombinases are key enzymes that catalyse the strand transfer step during recombination. RecA, the chief recombinase found in Escherichia...