Aggarwal, A. K.; Wah, D. A.; Hirsch, J. A.; Dorner, L. F.; Schildkraut, I. (1997). "Structure of the multimodular endonuclease Fok1 bound to DNA". Nature...
5 KB (628 words) - 19:09, 2 December 2023
Initial TALEN studies used the wild-type FokI cleavage domain, but some subsequent TALEN studies also used FokI cleavage domain variants with mutations...
27 KB (3,148 words) - 19:42, 31 October 2024
double-strand breaks (DSBs) in target DNA by the restriction endonucleases (FokI and Cas), and the repair of DSBs through homology-directed recombination...
78 KB (9,361 words) - 01:20, 25 September 2024
Foki (Russian: Фоки) is a rural locality (a selo) and the administrative center of Fokinskoye Rural Settlement, Chaykovsky, Perm Krai, Russia. The population...
3 KB (84 words) - 08:53, 22 October 2024
Restriction enzyme (redirect from Deoxyribonucleases, type i site-specific)
restriction endonucleases, such as DpnI, are able to recognize and cut methylated DNA. Type IIS restriction endonucleases (e.g. FokI) cleave DNA at a defined distance...
55 KB (5,853 words) - 14:21, 27 September 2024
non-specific cleavage domain from the type IIs restriction endonuclease FokI is typically used as the cleavage domain in ZFNs. This cleavage domain must...
36 KB (4,665 words) - 00:56, 23 September 2024
Timothy Fok Tsun-ting (born 14 February 1946), GBS, JP, the eldest son of Henry Fok, is the President of the Hong Kong Football Association. He formerly...
13 KB (1,058 words) - 21:32, 4 October 2023
are typically created through the fusion of Zinc-finger domains and the FokI nuclease domain. ZFNs have thus the ability to cleave DNA at target sites...
55 KB (6,765 words) - 09:23, 25 May 2024
also requires transition molecules as well as the FokI enzyme to be present. The requirement for the FokI enzyme limits application in vivo, at least for...
43 KB (4,916 words) - 23:10, 23 July 2024
made þaim kaiba i bormoþa huni A2 huwaʀ ob kam harie a hit lat A3 gotna fiskʀ oʀ firnauim suwimade foki af (f)a(nwan)ga lande B a(i a)u is urki C1 ni...
9 KB (1,277 words) - 15:24, 21 January 2024
okeanokoites is the original source for the type IIs restriction endonuclease FokI, used in Zinc finger nucleases and TALENs. Species who are a part of the...
23 KB (826 words) - 17:33, 31 March 2024
cleavage domain (usually the cleavage domain of FokI) to generate zinc finger nucleases. Such zinc finger-FokI fusions have become useful reagents for manipulating...
42 KB (4,879 words) - 13:27, 9 October 2024
monomers in which each subunit contains a zinc domain and a FokI endonuclease domain. The FokI domains must dimerize for activities, thus narrowing target...
71 KB (8,294 words) - 16:50, 27 September 2024
is the creation of artificial restriction enzymes created by linking the FokI DNA cleavage domain with an array of DNA binding proteins or zinc finger...
20 KB (2,668 words) - 02:28, 20 June 2024
PMID 15458627. Bitinaite J, Wah DA, Aggarwal AK, Schildkraut I (September 1998). "FokI dimerization is required for DNA cleavage". Proceedings of the...
8 KB (891 words) - 15:42, 23 July 2024
another nuclease enzyme from E. coli. FokI, a nuclease enzyme from Flavobacterium okeanokoites Schildkraut I, Banner CD, Rhodes CS, Parekh S (1984)....
5 KB (506 words) - 00:49, 14 June 2024
and VDR association with progression to AIDS follows an additive model. FokI polymorphism is a risk factor for enveloped virus infection as revealed in...
22 KB (2,646 words) - 05:40, 13 July 2024
subsequent infections. Hence these sequences play a key role in the antiviral (i.e. anti-phage) defense system of prokaryotes and provide a form of heritable...
126 KB (13,352 words) - 03:19, 27 October 2024
1365-313X.1999.00526.x. PMID 10476082. Mandecki W, Bolling TJ (August 1988). "FokI method of gene synthesis". Gene. 68 (1): 101–7. doi:10.1016/0378-1119(88)90603-8...
61 KB (7,073 words) - 16:39, 29 April 2024
dimerization-dependent FokI nuclease domains, used in ZFNs and TALENs, with the simplicity of CRISPR-cas9 has been developed. The FokI nuclease was originally...
53 KB (6,846 words) - 19:05, 8 July 2024
REACH ROME QUARTER-FINALS". www.eurosport.com. Retrieved 19 December 2021. "Foki Sinks Ruud In Epic Five-Setter". www.atptour.com. 4 June 2021. Retrieved...
44 KB (2,715 words) - 13:54, 21 October 2024
CRISPR-Gold - CRISPRa - CRISPRi Easi-CRISPR - FACE Enzyme Cas9 - FokI - EcoRI - PstI - SmaI HaeIII - Cas12a (Cpf1) - xCas9 Applications CAMERA - ICE - Genética...
22 KB (2,739 words) - 07:27, 29 September 2024
2011). "TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain". Nucleic Acids Research. 39 (1): 359–72. doi:10.1093/nar/gkq704...
134 KB (14,252 words) - 07:39, 2 November 2024
in human cells. CRISPR-Cas systems are separated into two classes: Class I, in which several Cas proteins associate with a crRNA to build a functional...
14 KB (1,591 words) - 00:23, 30 October 2024
pluripotency in human and mouse cells providing an alternative method to iPS technology. In addition, large-scale activation screens could be used to...
24 KB (2,898 words) - 12:08, 22 March 2024
PMID 8577732. Bitinaite J, Wah DA, Aggarwal AK, Schildkraut I (September 1998). "FokI dimerization is required for DNA cleavage". Proc. Natl. Acad....
21 KB (2,673 words) - 19:04, 28 September 2024
com. Engineered TAL effectors can also be fused to the cleavage domain of FokI to create TAL effector nucleases (TALEN) or to meganucleases (nucleases with...
28 KB (2,796 words) - 03:03, 9 November 2023
2011). "TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain". Nucleic Acids Research. 39 (1): 359–72. doi:10.1093/nar/gkq704...
102 KB (11,029 words) - 22:47, 3 November 2024
2011). "TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain". Nucleic Acids Research. 39 (1): 359–72. doi:10.1093/nar/gkq704...
144 KB (15,863 words) - 07:41, 2 November 2024
PLACE IN THE FINALS". www.daviscup.com. Retrieved 27 March 2023. "Emotional Foki Consoles Injured Friend Humbert In Montpellier". www.atptour.com. 8 February...
92 KB (6,727 words) - 00:43, 4 November 2024