• Thumbnail for Northern blot
    The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a...
    17 KB (2,120 words) - 20:20, 24 October 2024
  • Thumbnail for Blot (biology)
    electrophoresis prior to blotting. Southern blot for DNA northern blot for RNA reverse northern blot for RNA western blot for proteins far-western blot for protein–protein...
    6 KB (694 words) - 21:55, 28 March 2024
  • The reverse northern blot is a method by which gene expression patterns may be analyzed by comparing isolated RNA molecules from a tester sample to samples...
    10 KB (1,189 words) - 21:45, 10 November 2022
  • Thumbnail for Southern blot
    first published it in 1975. Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles,...
    17 KB (2,309 words) - 13:53, 2 October 2024
  • Thumbnail for Western blot
    The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics...
    66 KB (8,092 words) - 16:32, 2 November 2024
  • for the hybridisation of blotted DNA. Patricia Thomas, developer of the RNA blot which then became known as the northern blot, actually did not use the...
    49 KB (5,538 words) - 11:30, 2 November 2024
  • The northwestern blot, also known as the northwestern assay, is a hybrid analytical technique of the western blot and the northern blot, and is used in...
    11 KB (1,430 words) - 12:12, 5 June 2023
  • Thumbnail for Blót
    Blót (Old Norse and Old English) or geblōt (Old English) are religious ceremonies in Germanic paganism that centred on the killing and offering of an animal...
    57 KB (6,269 words) - 09:07, 1 November 2024
  • washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of...
    992 bytes (101 words) - 22:18, 16 January 2022
  • Thumbnail for Gene expression
    degree of multiplexity. Low-to-mid-plex techniques: Reporter gene Northern blot Western blot Fluorescent in situ hybridization Reverse transcription PCR Higher-plex...
    102 KB (11,438 words) - 17:14, 31 October 2024
  • Thumbnail for Reverse transcription polymerase chain reaction
    reverse transcription PCR (RT-PCR). Since its introduction in 1977, Northern blot has been used extensively for RNA quantification despite its shortcomings:...
    44 KB (5,656 words) - 23:37, 29 October 2024
  • Thumbnail for Real-time polymerase chain reaction
    abundance: differential display, RNase protection assay and northern blot. Northern blotting is often used to estimate the expression level of a gene by...
    43 KB (5,346 words) - 03:57, 30 October 2024
  • Thumbnail for Salmonella enterica
    discovered by deep sequencing and its transcription was confirmed by Northern blot and RACE analysis. AsdA is estimated to be about 540 nucleotides long...
    17 KB (1,911 words) - 09:16, 20 June 2024
  • Thumbnail for N,N-Dimethyltryptamine
    a gene encoding INMT is determined to be located on chromosome 7. Northern blot analyses reveal INMT messenger RNA (mRNA) to be highly expressed in...
    146 KB (14,500 words) - 09:07, 2 November 2024
  • Thumbnail for Blood test
    but is now also part of the diagnostic process of many disorders. Northern blot (RNA) Sexually transmitted diseases Full blood count (or "Complete Blood...
    16 KB (1,337 words) - 17:26, 26 July 2024
  • Thumbnail for Ob/ob mouse
    sequenced and searched in the GenBank. One of the exons was hybridized to a Northern blot of mouse white adipose tissue (WAT). This allowed to investigate the...
    4 KB (449 words) - 02:02, 16 February 2024
  • fluorescent protein vector Enzyme assay Protein purification Western blot Northern blot Southern blot Restriction enzyme Polymerase chain reaction Two-hybrid screening...
    82 KB (9,873 words) - 17:58, 31 October 2024
  • Thumbnail for Edwin Southern
    College, Oxford. He is most widely known for the invention of the Southern blot, published in 1975 and now a common laboratory procedure. Southern was born...
    13 KB (1,065 words) - 17:08, 19 June 2024
  • The eastern blot, or eastern blotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids...
    18 KB (2,101 words) - 01:17, 22 June 2024
  • Thumbnail for Denaturation (biochemistry)
    acid denaturation, the following methods were created: PCR Southern blot Northern blot DNA sequencing Acidic protein denaturants include: Acetic acid Trichloroacetic...
    30 KB (3,182 words) - 02:16, 27 October 2024
  • with phosphorus-32. This technique is extensively used in Southern blot and Northern blot analysis of DNA and RNA samples respectively. In both cases, a...
    7 KB (881 words) - 17:52, 10 October 2024
  • Thumbnail for Plasmodium RUF1 RNA
    plasmodium genome and the expression of this ncRNA was verified by northern blot. The location of this ncRNAs was subsequently mapped within the P. falciparum...
    2 KB (154 words) - 07:25, 10 February 2023
  • Kalogeris, T., Grisham, M. B., Alexander, J. S. (1997) An Improved, Rapid Northern Protocol. Biochem. and Biophys. Research Comm. 238:277–279. "Paper chromatography...
    18 KB (513 words) - 01:21, 22 October 2024
  • Thumbnail for Vibrio
    used in discoveries of many bacterial small RNAs. Using sRNA-Seq and Northern blot candidate sRNAs were identified and characterised as IGR-sRNA (intragenic...
    32 KB (2,852 words) - 00:56, 21 August 2024
  • Thumbnail for Polymerase chain reaction
    many methods, such as generating hybridization probes for Southern or northern blot hybridization. PCR supplies these techniques with large amounts of pure...
    89 KB (11,135 words) - 11:52, 26 August 2024
  • Thumbnail for Agarose gel electrophoresis
    Immunodiffusion, Immunoelectrophoresis SDD-AGE Northern blot SDS-polyacrylamide gel electrophoresis Southern blot Kryndushkin DS, Alexandrov IM, Ter-Avanesyan...
    34 KB (4,138 words) - 15:43, 31 October 2024
  • Thumbnail for Outline of biochemistry
    blot – fractionation – Gram stain – Surface Plasmon Resonance – Microscale Thermophoresis DNA sequencing Polymerase chain reaction Northern blotting Southern...
    8 KB (703 words) - 10:12, 26 June 2024
  • by Northern blotting or by DNA sequencing of a deletion allele), and one that produces no protein is called a protein null (shown by Western blotting)....
    11 KB (1,506 words) - 14:32, 22 March 2024
  • Thumbnail for Teg49 small RNA
    accessory regulator sarA. It was identified by RNA-seq and confirmed by Northern blot. It is modulated by sigB (sarA regulator) and cshA (an ATP-dependant...
    2 KB (206 words) - 18:05, 17 February 2024
  • Thumbnail for Wiskott–Aldrich syndrome
    detected on genetic testing, OR Absent or reduced WAS expression via Northern blot analysis of lymphocytes from a fresh blood sample, OR Absent or reduced...
    37 KB (3,794 words) - 17:22, 24 January 2024