Protein tags are peptide sequences genetically grafted onto a recombinant protein. Tags are attached to proteins for various purposes. They can be added...
24 KB (2,689 words) - 12:06, 9 July 2024
A polyhistidine-tag, best known by the trademarked name His-tag, is an amino acid motif in proteins that typically consists of at least six histidine...
22 KB (2,645 words) - 02:06, 22 June 2024
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a peptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence...
7 KB (846 words) - 23:15, 23 May 2024
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be...
2 KB (296 words) - 22:15, 4 April 2024
Look up Tag, TAG, tag, tág, tåg, or tagging in Wiktionary, the free dictionary. Tag, TAG, or tagging may refer to: Tag (graffiti), a form of graffiti signature...
5 KB (681 words) - 07:11, 13 July 2024
HaloTag is a self-labeling protein tag. It is a 297 residue protein (33 kDa) derived from a bacterial enzyme, designed to covalently bind to a synthetic...
9 KB (977 words) - 10:34, 27 December 2023
of the recombinant protein. This tag facilitates the detection, isolation and purification of the protein of interest. The HA-tag is not suitable for...
3 KB (398 words) - 10:45, 15 May 2024
SNAP-tag® is a self-labeling protein tag commercially available in various expression vectors. SNAP-tag is a 182 residue polypeptide (19.4 kDa) that can...
9 KB (1,001 words) - 20:35, 13 June 2024
The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide...
8 KB (1,027 words) - 04:19, 22 June 2024
biology research in order to track protein interactions in real time. The first fluorescent tag, green fluorescent protein (GFP), was isolated from Aequorea...
19 KB (2,305 words) - 05:22, 15 May 2024
The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP...
63 KB (7,460 words) - 06:00, 13 July 2024
desired protein (this is known as an expression system). Recombinant expression allows the protein to be tagged, e.g. by a His-tag or Strep-tag to facilitate...
30 KB (3,919 words) - 03:34, 22 June 2024
in the detection of a biomolecule such as a protein, antibody, or amino acid. Generally, fluorescent tagging, or labeling, uses a reactive derivative of...
24 KB (2,775 words) - 15:49, 8 January 2024
absorption-Shifting Tag) is a genetically-encoded protein tag which, upon reversible combination with a fluorogenic chromophore, allows the reporting of proteins of interest...
17 KB (1,827 words) - 16:29, 25 June 2024
Affinity chromatography (section Recombinant proteins)
metabolite protein. Dye ligand media is nonspecific but mimics biological substrates and proteins. Glutathione is useful for separation of GST tagged recombinant...
29 KB (3,430 words) - 03:30, 24 June 2024
Expression vector (section Protein tags)
Pasker (24 September 2013). "Overview of Affinity Tags for Protein Purification". Current Protocols in Protein Science. 73 (Unit-9.9): 9.9.1–9.9.23. doi:10...
34 KB (4,178 words) - 01:07, 24 March 2024
MS2 tagging is a technique based upon the natural interaction of the MS2 bacteriophage coat protein with a stem-loop structure from the phage genome,...
6 KB (746 words) - 17:06, 29 January 2023
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions...
101 KB (11,357 words) - 04:24, 10 July 2024
Protein splicing is an intramolecular reaction of a particular protein in which an internal protein segment (called an intein) is removed from a precursor...
31 KB (3,821 words) - 22:43, 17 December 2023
Epitope (section Epitope tags)
used for this purpose are Myc-tag, HA-tag, FLAG-tag, GST-tag, 6xHis, V5-tag and OLLAS. Peptides can also be bound by proteins that form covalent bonds to...
21 KB (2,397 words) - 06:13, 26 February 2024
Peptide (SBP)-Tag is a 38-amino acid sequence that may be engineered into recombinant proteins. Recombinant proteins containing the SBP-Tag bind to streptavidin...
16 KB (1,719 words) - 15:57, 16 January 2024
SpyCatcher (category Proteins)
The SpyTag/SpyCatcher system is a technology for irreversible conjugation of recombinant proteins. The peptide SpyTag (13 amino acids) spontaneously reacts...
23 KB (2,514 words) - 03:48, 4 January 2024
change. Fusion proteins are made by inserting protein tags, such as the His-tag, to produce a modified protein that is easier to track. An example of this...
10 KB (1,069 words) - 18:26, 11 December 2023
Tandem affinity purification (redirect from TAP-tag)
protein of interest to bind to the beads, it is tagged with a designed piece, the TAP tag. The original TAP method involves the fusion of the TAP tag...
10 KB (1,443 words) - 20:03, 3 January 2023
CUT&Tag-sequencing, also known as cleavage under targets and tagmentation, is a method used to analyze protein interactions with DNA. CUT&Tag-sequencing...
11 KB (1,292 words) - 20:59, 7 January 2024
Glutathione S-transferase (redirect from GST-tag)
the GST-fusion protein. The tag is 220 amino acids (roughly 26 kDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large...
38 KB (4,286 words) - 01:59, 22 June 2024
SV40 large T antigen (redirect from SV40 TAg)
(Simian Vacuolating Virus 40 TAg) is a hexamer protein that is a dominant-acting oncoprotein derived from the polyomavirus SV40. TAg is capable of inducing...
12 KB (1,413 words) - 20:11, 3 January 2023
purification of membrane proteins. The polyhistidine-tag is a commonly used tag for membrane protein purification, and the alternative rho1D4 tag has also been successfully...
18 KB (1,819 words) - 23:55, 25 April 2024
binding protein. Some of these tags may also allow for increased solubility of the target protein. The target protein is fused to the protein tag, but a...
18 KB (2,164 words) - 18:27, 4 June 2024
Peptide (section Protein-peptide interactions)
peptides, but with different properties. Protein tag, describing addition of peptide sequences to enable protein isolation or detection Replikins Ribosome...
22 KB (2,418 words) - 08:59, 12 July 2024