In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange...
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High-performance liquid chromatography (redirect from Gradient elution)
constant ("isocratic elution mode") or varied ("gradient elution mode") during the chromatographic analysis. Isocratic elution is typically effective...
89 KB (11,002 words) - 04:19, 2 September 2024
An antibody elution is a clinical laboratory diagnostic procedure which removes sensitized antibodies from red blood cells, in order to determine the...
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desorbed by an elution buffer. The mode used for elution (expanded-bed versus settled-bed) depends on the characteristics of the feed. After elution, the adsorbent...
59 KB (7,375 words) - 18:27, 3 September 2024
gel (stationary phase). To determine approximate molecular weight, the elution volumes of compounds with their corresponding molecular weights are obtained...
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molecules, thus separate them. This type of elution is called gradient elution. On the other hand, step elution can be used, in which the concentration of...
54 KB (6,991 words) - 02:26, 21 July 2024
pre-incubated drugs should mirror the elution profiles of the targets they complex with. Correlation of drug and protein elution profiles allows for targets to...
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elutes very late in the elution sequence, after fermium. Another method to isolate mendelevium exploits the distinct elution properties of Md2+ from those...
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who in 1943 first classified the modes of chromatography as frontal, elution, and displacement. Displacement chromatography found a variety of applications...
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Systematic evolution of ligands by exponential enrichment (section Binding sequence elution and amplification)
prepare for subsequent rounds of selection in which the stringency of the elution conditions can be increased to identify the tightest-binding sequences...
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retained while impurities are washed away. An elution with a low pH buffer or a more gentle, high salt elution buffer is then used to recover purified antibody...
49 KB (4,964 words) - 04:02, 9 August 2024
His-tag (section Elution method)
histidine and is typically used at a concentration of 150 - 500 mM for elution. Histidine or histamine can also be used. Decrease in pH When the pH decreases...
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bound. Target biomolecules may then be removed by applying a so-called elution buffer, which disrupts interactions between the bound target biomolecules...
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divided into solvent selectivity groups. Using solvents with different elution strengths or different selectivity groups can often give very different...
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Kleihauer–Betke ("KB") test, Kleihauer–Betke ("KB") stain, Kleihauer test or acid elution test is a blood test used to measure the amount of fetal hemoglobin transferred...
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Elution: chromatographic separation of Fm(100), Es(99), Cf, Bk, Cm and Am...
32 KB (4,023 words) - 03:49, 28 August 2024
known (see Mark–Houwink equation), a plot of log [η]M versus elution volume (or elution time) for a particular solvent, column and instrument provides...
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particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in...
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divalent state, unlike the other trivalent actinides: under typically used elution conditions (bis-(2-ethylhexyl) phosphoric acid (HDEHP) as stationary organic...
39 KB (8,151 words) - 20:49, 25 August 2024
binding of biotinylated DNA to streptavidin-coated magnetic beads and elution of single DNA strands selectively using alkali. The method allowed robotic...
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in water, and passed down a column of cellulose phosphate. A subsequent elution with ammonium hydroxide and recrystallization from alcohol results in pure...
37 KB (3,826 words) - 05:00, 11 August 2024
these materials and are afterwards desorbed either thermally (Tenax) or by elution (DNPH) and then analyzed by GC–MS/FID or HPLC. Reference gas mixtures are...
60 KB (6,530 words) - 04:03, 2 September 2024
X-ray fluorescence (XRF). Before the time that ion exchange methods and elution were available, the separation of the rare earths was primarily achieved...
156 KB (16,786 words) - 00:07, 30 August 2024
Activated carbon with particle size less than 1 mm would not be suitable for elution (the stripping of mineral from an activated carbon). Acid-base, oxidation-reduction...
75 KB (8,332 words) - 15:05, 30 August 2024
as a pharmaceutical is chemical, and the radionuclide is extracted by elution from germanium-68, a synthetic radioisotope of germanium, in gallium-68...
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chromatogram. The separate curves in the diagram represent different sample elution concentration profiles over time based on their affinity to the column...
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the result of the first application of rate theory to the chromatography elution process. The van Deemter equation relates height equivalent to a theoretical...
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following fractions were designated HbA1a, HbA1b, and HbA1c, in their order of elution. Improved separation techniques have subsequently led to the isolation...
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of 256Lr precluded a confirmation that it eluted ahead of Md3+ in the elution sequence. Lawrencium occurs as the trivalent Lr3+ ion in aqueous solution...
41 KB (8,137 words) - 05:45, 3 September 2024
wool and silks, a colorant in food, drugs and cosmetics and an adsorption-elution indicator for chloride estimations in biochemistry. Many foods contain...
24 KB (2,459 words) - 16:06, 15 July 2024