Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication...
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being the first mammal cloned from a somatic cell. Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a...
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"Foundations of Molecular Cloning - Past, Present and Future | NEB". www.neb.com. Retrieved 2021-11-25. "Foundations of Molecular Cloning - Past, Present...
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Golden Gate Cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple...
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Gibson assembly (redirect from Gibson cloning)
Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after...
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In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence...
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and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism...
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action of an enzyme. It is an essential laboratory procedure in the molecular cloning of DNA, whereby DNA fragments are joined to create recombinant DNA...
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Recombinant DNA (category Molecular genetics)
molecules formed by laboratory methods of genetic recombination (such as molecular cloning) that bring together genetic material from multiple sources, creating...
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Tom Maniatis (category American molecular biologists)
involved in the writing of the “Molecular Cloning Manual”. In 1979, the Maniatis lab had developed and deployed gene cloning methods. Maniatis and Ed Fritsch...
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Blue–white screen (section Molecular mechanism)
and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a...
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associated cloning data to non-profit and academic laboratories around the world. Addgene provides a free online database of plasmid cloning information...
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Cloning is the process of making an identical copy of something. Cloning may also refer to: Cloning (programming), the copying of a programming object...
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Topoisomerase-based cloning (TOPO cloning) is a molecular biology technique in which DNA fragments are cloned into specific vectors without the requirement...
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Genetics (section Molecular genetics)
circular DNA molecules with a few genes on them. In the process known as molecular cloning, researchers can amplify the DNA fragments by inserting plasmids into...
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Functional cloning is a molecular cloning technique that relies on prior knowledge of the encoded protein’s sequence or function for gene identification...
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Shimoda-Takano K, Ishikawa H, Sakakibara S, et al. (June 1991). "Molecular cloning of cDNA encoding adipogenesis inhibitory factor and identity with...
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Restriction enzyme (redirect from Molecular scissors)
for DNA modification in laboratories, and they are a vital tool in molecular cloning. The term restriction enzyme originated from the studies of phage...
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Liu, Xiangqin; Li, Xue; Mi, Junpeng; Meng, Qing (1 October 2018). "Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus...
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Subcloning (redirect from Sub-cloning)
sequencing. Cloning Molecular cloning Polymerase chain reaction TA cloning Williams, Steven A.; et al. (2006). Laboratory Investigations in Molecular Biology...
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Hayashizaki Y, Takahashi Y, Himeno S, Matsubara K (December 1983). "Molecular cloning of the human gastrin gene". Nucleic Acids Research. 11 (23): 8197–203...
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Library (biology) (redirect from Clone library)
and propagated in a population of microbes through the process of molecular cloning. There are different types of DNA libraries, including cDNA libraries...
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Maniatis T, Fritsch EF, Sambrook J (1982). "Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. Vol. 1. p. 5.4. ISBN 978-0879691363. Stephen...
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Plasmid (category Molecular biology)
circumstances. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within...
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Overlap extension polymerase chain reaction (category Molecular biology)
based cloning methos such as Gibson assembly, which overcome the limitations of traditional restriction enzyme digestion and ligation cloning methods...
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against separation. Barrere GC, Barber CE, Daniels MJ (December 1986). "Molecular cloning of genes involved in the production of the extracellular polysaccharide...
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Poliovirus (section Cloning and synthesis)
E, Racaniello VR (March 1989). "Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin...
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editions of the best-selling, highly influential laboratory manual Molecular Cloning, the third in 2001 with David Russell and the fourth in 2012 with...
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use in molecular biology laboratories for recombinant DNA experiments (see Research applications). Purified DNA ligase is used in gene cloning to join...
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Gateway Technology (redirect from Gateway cloning)
The Gateway cloning method is a method of molecular cloning invented and commercialized by Invitrogen since the late 1990s, which makes use of the integration...
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