• Thumbnail for Molecular cloning
    Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication...
    32 KB (4,016 words) - 17:56, 20 August 2024
  • Thumbnail for Cloning
    being the first mammal cloned from a somatic cell. Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a...
    107 KB (11,557 words) - 19:45, 12 August 2024
  • "Foundations of Molecular Cloning - Past, Present and Future | NEB". www.neb.com. Retrieved 2021-11-25. "Foundations of Molecular Cloning - Past, Present...
    49 KB (5,528 words) - 13:46, 18 August 2024
  • Thumbnail for Golden Gate Cloning
    Golden Gate Cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple...
    18 KB (2,489 words) - 05:22, 30 April 2024
  • Gibson assembly is a molecular cloning method that allows for the joining of multiple DNA fragments in a single, isothermal reaction. It is named after...
    6 KB (689 words) - 15:42, 24 May 2024
  • In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence...
    18 KB (2,164 words) - 18:27, 4 June 2024
  • Thumbnail for Cloning vector
    and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism...
    20 KB (2,517 words) - 08:10, 5 October 2023
  • Thumbnail for Ligation (molecular biology)
    action of an enzyme. It is an essential laboratory procedure in the molecular cloning of DNA, whereby DNA fragments are joined to create recombinant DNA...
    36 KB (4,679 words) - 19:39, 16 August 2024
  • Thumbnail for Recombinant DNA
    Recombinant DNA (category Molecular genetics)
    molecules formed by laboratory methods of genetic recombination (such as molecular cloning) that bring together genetic material from multiple sources, creating...
    35 KB (3,893 words) - 21:59, 18 August 2024
  • Tom Maniatis (category American molecular biologists)
    involved in the writing of the “Molecular Cloning Manual”. In 1979, the Maniatis lab had developed and deployed gene cloning methods. Maniatis and Ed Fritsch...
    20 KB (1,883 words) - 00:44, 6 April 2024
  • Thumbnail for Blue–white screen
    and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a...
    13 KB (1,821 words) - 00:36, 3 December 2023
  • associated cloning data to non-profit and academic laboratories around the world. Addgene provides a free online database of plasmid cloning information...
    10 KB (756 words) - 18:49, 5 March 2024
  • Cloning is the process of making an identical copy of something. Cloning may also refer to: Cloning (programming), the copying of a programming object...
    725 bytes (133 words) - 19:24, 23 May 2020
  • Topoisomerase-based cloning (TOPO cloning) is a molecular biology technique in which DNA fragments are cloned into specific vectors without the requirement...
    4 KB (568 words) - 01:06, 18 June 2024
  • Thumbnail for Genetics
    circular DNA molecules with a few genes on them. In the process known as molecular cloning, researchers can amplify the DNA fragments by inserting plasmids into...
    98 KB (10,412 words) - 15:40, 28 August 2024
  • Thumbnail for Functional cloning
    Functional cloning is a molecular cloning technique that relies on prior knowledge of the encoded protein’s sequence or function for gene identification...
    25 KB (3,005 words) - 15:19, 1 July 2024
  • Thumbnail for Interleukin 11
    Shimoda-Takano K, Ishikawa H, Sakakibara S, et al. (June 1991). "Molecular cloning of cDNA encoding adipogenesis inhibitory factor and identity with...
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  • for DNA modification in laboratories, and they are a vital tool in molecular cloning. The term restriction enzyme originated from the studies of phage...
    55 KB (5,853 words) - 18:09, 20 August 2024
  • Thumbnail for Araneus ventricosus
    Liu, Xiangqin; Li, Xue; Mi, Junpeng; Meng, Qing (1 October 2018). "Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus...
    14 KB (1,552 words) - 19:09, 21 August 2024
  • Thumbnail for Subcloning
    Subcloning (redirect from Sub-cloning)
    sequencing. Cloning Molecular cloning Polymerase chain reaction TA cloning Williams, Steven A.; et al. (2006). Laboratory Investigations in Molecular Biology...
    5 KB (628 words) - 02:32, 5 February 2024
  • Thumbnail for Gastrin
    Hayashizaki Y, Takahashi Y, Himeno S, Matsubara K (December 1983). "Molecular cloning of the human gastrin gene". Nucleic Acids Research. 11 (23): 8197–203...
    21 KB (2,424 words) - 18:04, 23 April 2024
  • Thumbnail for Library (biology)
    and propagated in a population of microbes through the process of molecular cloning. There are different types of DNA libraries, including cDNA libraries...
    11 KB (1,204 words) - 21:21, 22 April 2024
  • Thumbnail for Agarose
    Maniatis T, Fritsch EF, Sambrook J (1982). "Chapter 5, protocol 1". Molecular Cloning - A Laboratory Manual. Vol. 1. p. 5.4. ISBN 978-0879691363. Stephen...
    19 KB (2,406 words) - 08:48, 8 July 2024
  • Thumbnail for Plasmid
    Plasmid (category Molecular biology)
    circumstances. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within...
    46 KB (5,311 words) - 15:40, 29 June 2024
  • Overlap extension polymerase chain reaction (category Molecular biology)
    based cloning methos such as Gibson assembly, which overcome the limitations of traditional restriction enzyme digestion and ligation cloning methods...
    8 KB (1,051 words) - 12:10, 14 June 2024
  • Thumbnail for Xanthan gum
    against separation. Barrere GC, Barber CE, Daniels MJ (December 1986). "Molecular cloning of genes involved in the production of the extracellular polysaccharide...
    15 KB (1,694 words) - 07:08, 14 July 2024
  • Thumbnail for Poliovirus
    E, Racaniello VR (March 1989). "Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin...
    53 KB (6,663 words) - 18:47, 12 August 2024
  • editions of the best-selling, highly influential laboratory manual Molecular Cloning, the third in 2001 with David Russell and the fourth in 2012 with...
    8 KB (656 words) - 14:21, 6 September 2023
  • Thumbnail for DNA ligase
    use in molecular biology laboratories for recombinant DNA experiments (see Research applications). Purified DNA ligase is used in gene cloning to join...
    23 KB (2,787 words) - 00:53, 18 June 2024
  • The Gateway cloning method is a method of molecular cloning invented and commercialized by Invitrogen since the late 1990s, which makes use of the integration...
    9 KB (1,359 words) - 04:00, 18 August 2024