The versatility of polymerase chain reaction (PCR) has led to modifications of the basic protocol being used in a large number of variant techniques designed...

The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists...

reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to...

between RT-PCR and qPCR has led to metonymic use of the term qPCR to mean RT-PCR. Such use may be confusing, as RT-PCR can be used without qPCR, for example...

Vectorette PCR was first noted and described in an article in 1990 by John H. Riley and his team. Since then, multiple variants of PCR have been created...

by some parts of France). A third and fourth RT-PCR test intended for detecting specific variants pre-screen the samples for variants respectively with...

(real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors...

Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods...

of which focus on specific applications of PCR. Primers with high specificity for a subset of DNA templates in the presence of many similar variants can...

individuals. Variants that appear to meet one or more of these criteria may be labelled "variants under investigation" or "variants of interest" pending...

through a column of absorbent material. DNA analysis using polymerase chain reaction (PCR), to amplify small samples of DNA. Variants of PCR used to diagnose...

samples from the very beginning of November where the PCR tests had S gene target failure (occurs in a few variants, but not in Delta which dominated...

of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR)...

(Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is...

COLD-PCR (co-amplification at lower denaturation temperature PCR) is a modified polymerase chain reaction (PCR) protocol that enriches variant alleles...

the course of PCR optimisation. In addition to PCR, thermostable DNA polymerases are also used for RT-PCR variants, qPCR in different variants, site-specific...

B.1.617.2 genome. As of August 2021, Delta variants have been subdivided in the Pango lineage designation system into variants from AY.1 to AY.28. However...

spotted microarrays, the probes are oligonucleotides, cDNA or small fragments of PCR products that correspond to mRNAs. The probes are synthesized prior to deposition...

portal Variants of SARS-CoV-2: Alpha, Beta, Gamma, Delta, Epsilon, Zeta, Theta, Iota, Kappa, Lambda, Mu, Omicron "Delta-PCR-testen" [The Delta PCR Test]...

that amplify the variant of interest more efficiently than wildtype sequence. PCR is currently the most widely used method for detection of DNA sequences...

used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments of DNA. T. aquaticus is a bacterium that lives...

Hot start PCR is a modified form of conventional polymerase chain reaction (PCR) that reduces the presence of undesired products and primer dimers due...

g. by error-prone PCR or sequence saturation mutagenesis, is applied to a protein, and a selection regime is used to select variants having desired traits...

Reverse complement polymerase chain reaction (RC-PCR) is a modification of the polymerase chain reaction (PCR). It is primarily used to generate amplicon libraries...

Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments...

cancer cells. DNA variants must also commonly be taken into consideration in molecular diagnostics applications such as designing PCR primers to detect...

polymerase is combined with selective PCR amplification to produce many copies of mutant DNA. RT-PCR differs from conventional PCR as it synthesizes cDNA from mRNA...

However, the operational range of structural variants has widened to include events > 50bp. Some structural variants are associated with genetic diseases...

"amplicon reads," are created following the removal of erroneous sequences generated during PCR and sequencing, using ASVs makes it possible to distinguish...

methylation-specific PCR. Following PCR amplification of the region of interest, pyrosequencing is used to determine the bisulfite-converted sequence of specific...